Brent Segelke Physical and Life Sciences Directorate Lawrence Livermore National Laboratory Biography Publications Institution JoVE Articles Brent Segelke has not added a biography. If you are Brent Segelke and would like to personalize this page please email our Author Liaison for assistance. Publications Functional Annotation from Structural Homology Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34718998 Shotgun Immunoproteomic Approach for the Discovery of Linear B-Cell Epitopes in Biothreat Agents and Frontiers in Immunology. 2021 | Pubmed ID: 34659206 Discovery of Small-Molecule Inhibitors of SARS-CoV-2 Proteins Using a Computational and Experimental Pipeline Frontiers in Molecular Biosciences. 2021 | Pubmed ID: 34327214 Proteomic Profiling of During Host Infection Using Bio-Orthogonal Noncanonical Amino Acid Tagging (BONCAT) Frontiers in Cellular and Infection Microbiology. 2018 | Pubmed ID: 30406044 Resolution Extension by Image Summing in Serial Femtosecond Crystallography of Two-dimensional Membrane-protein Crystals IUCrJ. Jan, 2018 | Pubmed ID: 29354276 Expression and Association of the Yersinia Pestis Translocon Proteins, YopB and YopD, Are Facilitated by Nanolipoprotein Particles PloS One. 2016 | Pubmed ID: 27015536 Structure and Function of REP34 Implicates Carboxypeptidase Activity in Francisella Tularensis Host Cell Invasion The Journal of Biological Chemistry. Oct, 2014 | Pubmed ID: 25231992 Fixed-target Protein Serial Microcrystallography with an X-ray Free Electron Laser Scientific Reports. Aug, 2014 | Pubmed ID: 25113598 Femtosecond X-ray Diffraction from Two-dimensional Protein Crystals IUCrJ. Mar, 2014 | Pubmed ID: 25075325 7 Å Resolution in Protein Two-dimensional-crystal X-ray Diffraction at Linac Coherent Light Source Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. Jul, 2014 | Pubmed ID: 24914166 Practical Applications of Structural Genomics Technologies for Mutagen Research Mutation Research. Jun, 2011 | Pubmed ID: 21182983 Isolation, Characterization, and Stability of Discretely-sized Nanolipoprotein Particles Assembled with Apolipophorin-III PloS One. Jul, 2010 | Pubmed ID: 20657844 Structure of Rv1848 (UreA), the Mycobacterium Tuberculosis Urease Gamma Subunit Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jul, 2010 | Pubmed ID: 20606272 An Extracellular Disulfide Bond Forming Protein (DsbF) from Mycobacterium Tuberculosis: Structural, Biochemical, and Gene Expression Analysis Journal of Molecular Biology. Mar, 2010 | Pubmed ID: 20060836 Amino Acid and Structural Variability of Yersinia Pestis LcrV Protein Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases. Jan, 2010 | Pubmed ID: 19835996 Characterization and Purification of Polydisperse Reconstituted Lipoproteins and Nanolipoprotein Particles International Journal of Molecular Sciences. Jul, 2009 | Pubmed ID: 19742178 Immobilization of His-tagged Proteins on Nickel-chelating Nanolipoprotein Particles Bioconjugate Chemistry. Mar, 2009 | Pubmed ID: 19239247 Atomic Force Microscopy Differentiates Discrete Size Distributions Between Membrane Protein Containing and Empty Nanolipoprotein Particles Biochimica Et Biophysica Acta. Mar, 2009 | Pubmed ID: 19109924 Cell-free Expression for Nanolipoprotein Particles: Building a High-throughput Membrane Protein Solubility Platform Methods in Molecular Biology (Clifton, N.J.). 2009 | Pubmed ID: 18988032 Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles Molecular & Cellular Proteomics : MCP. Nov, 2008 | Pubmed ID: 18603642 Quantifying Size Distributions of Nanolipoprotein Particles with Single-particle Analysis and Molecular Dynamic Simulations Journal of Lipid Research. Jul, 2008 | Pubmed ID: 18403317 Different Apolipoproteins Impact Nanolipoprotein Particle Formation Journal of the American Chemical Society. Nov, 2007 | Pubmed ID: 17963384 Structure of Mycobacterium Tuberculosis RuvA, a Protein Involved in Recombination Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Aug, 2006 | Pubmed ID: 16880543 Macromolecular Crystallization with Microfluidic Free-interface Diffusion Expert Review of Proteomics. Apr, 2005 | Pubmed ID: 15892562 Laboratory Scale Structural Genomics Journal of Structural and Functional Genomics. 2004 | Pubmed ID: 15263853 Crystal Structure of Clostridium Botulinum Neurotoxin Protease in a Product-bound State: Evidence for Noncanonical Zinc Protease Activity Proceedings of the National Academy of Sciences of the United States of America. May, 2004 | Pubmed ID: 15107500 The High-speed Hydra-Plus-One System for Automated High-throughput Protein Crystallography Acta Crystallographica. Section D, Biological Crystallography. Oct, 2002 | Pubmed ID: 12351853 The TB Structural Genomics Consortium Crystallization Facility: Towards Automation from Protein to Electron Density Acta Crystallographica. Section D, Biological Crystallography. Oct, 2002 | Pubmed ID: 12351851 Structural Basis for Abrogated Binding Between Staphylococcal Enterotoxin A Superantigen Vaccine and MHC-IIalpha Protein Science : a Publication of the Protein Society. Mar, 2002 | Pubmed ID: 11847286 Бесклеточное масштабное производство и адъювантное добавление к рекомбинантному белку внешней мембраны из Chlamydia muridarum для разработки вакцины Sean F. Gilmore*1, Wei He*1, Angela C. Evans1, Delia F. Tifrea2, Sukumar Pal2, Brent Segelke1, Sandra K. G. Peters1, B. Dillon Vannest1, Nicholas O. Fischer1, Amy Rasley1, Luis M. de la Maza2, Matthew A. Coleman1,3 1Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 2Department of Pathology and Laboratory Medicine, University of California, 3School of Medicine, Radiation Oncology, University of California Davis JoVE 63028 Immunology and Infection
Бесклеточное масштабное производство и адъювантное добавление к рекомбинантному белку внешней мембраны из Chlamydia muridarum для разработки вакцины Sean F. Gilmore*1, Wei He*1, Angela C. Evans1, Delia F. Tifrea2, Sukumar Pal2, Brent Segelke1, Sandra K. G. Peters1, B. Dillon Vannest1, Nicholas O. Fischer1, Amy Rasley1, Luis M. de la Maza2, Matthew A. Coleman1,3 1Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, 2Department of Pathology and Laboratory Medicine, University of California, 3School of Medicine, Radiation Oncology, University of California Davis JoVE 63028 Immunology and Infection