Articles by Caitlin M. Klimavicz in JoVE
Luciferase immunoprecipitation सिस्टम (होंठ) द्वारा एंटीबॉडी रूपरेखा Peter D. Burbelo1, Kathryn H. Ching1, Caitlin M. Klimavicz1, Michael J. Iadarola1 1Neurobiology and Pain Therapeutics Section, National Institute of Dental and Craniofacial Research, National Institutes of Health होंठ (luciferase immunoprecipitation सिस्टम) प्रदर्शन के तकनीकी पहलुओं का वर्णन कर रहे हैं. समग्र दृष्टिकोण chimeric स्तनधारी कोशिकाओं में Renilla luciferase (Ruc) के लिए जुड़े हुए प्रतिजनों जीन एन्कोडिंग व्यक्त शामिल है. क्रूड Ruc प्रतिजन अर्क और फिर तैयार हैं, शुद्धि के बिना, immunoprecipitation assays में कार्यरत एंटीबॉडी यों.
Other articles by Caitlin M. Klimavicz on PubMed
Anti-cytokine Autoantibodies Are Associated with Opportunistic Infection in Patients with Thymic Neoplasia Blood. Dec, 2010 | Pubmed ID: 20716769 Patients with thymic malignancy have high rates of autoimmunity leading to a variety of autoimmune diseases, most commonly myasthenia gravis caused by anti-acetylcholine receptor autoantibodies. High rates of autoantibodies to cytokines have also been described, although prevalence, spectrum, and functionality of these anti-cytokine autoantibodies are poorly defined. To better understand the presence and function of anti-cytokine autoantibodies, we created a luciferase immunoprecipitation system panel to search for autoantibodies against 39 different cytokines and examined plasma from controls (n = 30) and patients with thymic neoplasia (n = 17). In this screen, our patients showed statistically elevated, but highly heterogeneous immunoreactivity against 16 of the 39 cytokines. Some patients showed autoantibodies to multiple cytokines. Functional testing proved that autoantibodies directed against interferon-Î±, interferon-Î², interleukin-1Î± (IL-1Î±), IL-12p35, IL-12p40, and IL-17A had biologic blocking activity in vitro. All patients with opportunistic infection showed multiple anti-cytokine autoantibodies (range 3-11), suggesting that anti-cytokine autoantibodies may be important in the pathogenesis of opportunistic infections in patients with thymic malignancy. This study was registered at http://clinicaltrials.gov as NCT00001355.
Synthetic Biology for Translational Research American Journal of Translational Research. 2010 | Pubmed ID: 20733948 Synthetic biology involves the engineering of proteins, signaling pathways and even whole organisms using modular designs and formats. A major tool of synthetic biology is artificial gene synthesis, which provides a direct means from a conceptualized DNA sequence to the corresponding physical DNA for the construction of a variety of biological components. To date, synthetic biology has often been used to answer fundamental questions in basic research, but now is poised to greatly enhance translational research. In this review, we discuss several translational applications of synthetic biology including the construction of novel diagnostics and vaccines, development of new synthetic pathways for drug screening and biosynthesis, and the creation of engineered viruses and microbes to fight human disease. Together these and other novel translational applications of artificial gene synthesis and synthetic biology have the opportunity to make major advances for improving human health.