Articles by Cemile G Guldal in JoVE
Анализ для адгезии и агар Вторжение в CEREVISIAE С. Cemile G Guldal1, James Broach1 1Department of Molecular Biology, Princeton University Мы описываем качественный анализ для адгезии дрожжей и агар вторжение в качестве меры инвазивных и pseudohyphal дифференциации. Этот простой анализ может быть использован для оценки инвазивного фенотипа различных мутантов, а также эффекты сигналами окружающей среды и сигнальных путей на дрожжах дифференциации.
Other articles by Cemile G Guldal on PubMed
Î²-Arrestin-1 Links Mitogenic Sonic Hedgehog Signaling to the Cell Cycle Exit Machinery in Neural Precursors Cell Cycle (Georgetown, Tex.). Oct, 2010 | Pubmed ID: 20935513 Development of the cerebellum, a brain region regulating posture and coordination, occurs post-natally and is marked by rapid proliferation of granule neuron precursors (CGNPs), stimulated by mitogenic Sonic hedgehog (Shh) signaling. Î²-Arrestin (Î²Arr) proteins play important roles downstream of Smoothened, the Shh signal transducer. However, whether Shh regulates Î²Arrs and what role they play in Shh-driven CGNP proliferation remains to be determined. Here, we report that Shh induces Î²Arr1 accumulation and localization to the nucleus, where it participates in enhancing expression of the cyclin dependent kinase (cdk) inhibitor p27, whose accumulation eventually drives CGNP cell cycle exit. Î²Arr1 knockdown enhances CGNP proliferation and reduces p27 expression. Thus, Shh-mediated Î²Arr1 induction represents a novel negative feedback loop within the Shh mitogenic pathway, such that ongoing Shh signaling, while required for CGNPs to proliferate, also sets up a cell-intrinsic clock programming their ultimate exit from the cell cycle.
An Essential Role for P38 MAPK in Cerebellar Granule Neuron Precursor Proliferation Acta Neuropathologica. Feb, 2012 | Pubmed ID: 22302101 Development of the cerebellum occurs postnatally and is marked by a rapid proliferation of cerebellar granule neuron precursors (CGNPs). CGNPs are the cells of origin for SHH-driven medulloblastoma, the most common malignant brain tumor in children. Here, we investigated the role of ERK, JNK, and p38 mitogen-activated protein kinases in CGNP proliferation. We found high levels of p38Î± in proliferating CGNPs. Concomitantly, members of the p38 pathway, such as ASK1, MKK3 and ATF-2, were also elevated. Inhibition of the Shh pathway or CGNP proliferation blunts p38Î± levels, irrespective of Shh treatment. Strikingly, p38Î± levels were high in vivo in the external granule layer of the postnatal cerebellum, Shh-dependent mouse medulloblastomas and human medulloblastomas of the SHH subtype. Finally, knocking down p38Î± by short hairpin RNA-carrying lentiviruses as well as the pharmacologically inhibiting of its kinase activity caused a marked decrease in CGNP proliferation, underscoring its requirement for Shh-dependent proliferation in CGNPs. The inhibition of p38Î± also caused a decrease in Gli1 and N-myc transcript levels, consistent with reduced proliferation. These findings suggest p38 inhibition as a potential way to increase the efficacy of treatments available for malignancies associated with deregulated SHH signaling, such as basal cell carcinoma and medulloblastoma.
Barriers in Accurate and Complete Birth Registration in New York State Maternal and Child Health Journal. Feb, 2015 | Pubmed ID: 25652064 Birth records have important legal, administrative and public health uses. However, invalid and incomplete birth reporting is a significant problem in New York State (NYS) and nationwide. We aimed to identify current practices and potential barriers in data collection by birth registrars (BRs) in NYS facilities. Using a 28-question survey regarding birth data collection, we surveyed 127 BRs in August 2013. The response rate was 88.2 % (n = 112), with 31.2 % from New York City (NYC) and 68.8 % from the Rest of State (ROS). NYC facilities were dedicating significantly fewer staff hours (0.98 h) per birth to electronic birth registration on average compared to facilities in the ROS (1.54 h/birth). ROS BRs reported significantly less support in continuing education/training for data quality, and supervisor or manager review, and significantly greater use of electronic reports to monitor data quality compared to NYC BRs. Fewer than half the BRs statewide reported being able to accurately report previous low-birthweight birth, previous preterm delivery, and date of last menses. In addition, NYC BRs reported being significantly less able to accurately report previous C-section, method of delivery, and birthweight compared to ROS BRs. Furthermore, NYC BRs had more problems with fetal presentation coding compared to ROS BRs. The implementation of good practices identified in this report and the elimination of barriers suggested by the results are being used to guide the development of statewide efforts to improve birth data accuracy and completeness.