Chase L. Beisel Helmholtz Institute for RNA-based Infection Research (HIRI) Helmholtz-Centre for Infection Research (HZI) Biography Publications Institution JoVE Articles Chase L. Beisel has not added a biography. If you are Chase L. Beisel and would like to personalize this page please email our Author Liaison for assistance. Publications A Target Expression Threshold Dictates Invader Defense and Prevents Autoimmunity by CRISPR-Cas13 Cell Host & Microbe. Jun, 2022 | Pubmed ID: 35690065 Reprogramming TracrRNAs for Multiplexed RNA Detection Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 35666448 Anti-CRISPR Prediction Using Deep Learning Reveals an Inhibitor of Cas13b Nucleases Molecular Cell. Jul, 2022 | Pubmed ID: 35649413 Genome Editing with Cas9 in Lactobacilli Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 35583743 Spacer Prioritization in CRISPR-Cas9 Immunity is Enabled by the Leader RNA Nature Microbiology. 04, 2022 | Pubmed ID: 35314780 Rapid Cell-free Characterization of Multi-subunit CRISPR Effectors and Transposons Molecular Cell. 03, 2022 | Pubmed ID: 35216669 A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34985758 Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34694607 Illuminating the Path to DNA Repair Cell. 10, 2021 | Pubmed ID: 34715020 The TracrRNA in CRISPR Biology and Technologies Annual Review of Genetics. 11, 2021 | Pubmed ID: 34416117 CRISPR Transposons on the Move Cell Host & Microbe. 05, 2021 | Pubmed ID: 33984272 Noncanonical CrRNAs Derived from Host Transcripts Enable Multiplexable RNA Detection by Cas9 Science (New York, N.Y.). 05, 2021 | Pubmed ID: 33906967 CRISPR Technologies and the Search for the PAM-free Nuclease Nature Communications. 01, 2021 | Pubmed ID: 33483498 Your Base Editor Might Be Flirting with Single (Stranded) DNA: Faithful On-Target CRISPR Base Editing Without Promiscuous Deamination Molecular Cell. 09, 2020 | Pubmed ID: 32888434 Methods for Characterizing, Applying, and Teaching CRISPR-Cas Systems Methods (San Diego, Calif.). 02, 2020 | Pubmed ID: 31954772 An Enhanced Assay to Characterize Anti-CRISPR Proteins Using a Cell-free Transcription-translation System Methods (San Diego, Calif.). 02, 2020 | Pubmed ID: 31121300 CRATES: A One-step Assembly Method for Class 2 CRISPR Arrays Methods in Enzymology. 2019 | Pubmed ID: 31727255 Modular One-pot Assembly of CRISPR Arrays Enables Library Generation and Reveals Factors Influencing CrRNA Biogenesis Nature Communications. 07, 2019 | Pubmed ID: 31270316 Barriers to Genome Editing with CRISPR in Bacteria Journal of Industrial Microbiology & Biotechnology. Oct, 2019 | Pubmed ID: 31165970 Cell-Free Protein Synthesis from Exonuclease-Deficient Cellular Extracts Utilizing Linear DNA Templates Mahnaz Sabeti Azad*1, Angelo Cardoso Batista*1, Jean-Loup Faulon1, Chase L. Beisel2,3, Jerome Bonnet4, Manish Kushwaha1 1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier JoVE 64236 Bioengineering
Cell-Free Protein Synthesis from Exonuclease-Deficient Cellular Extracts Utilizing Linear DNA Templates Mahnaz Sabeti Azad*1, Angelo Cardoso Batista*1, Jean-Loup Faulon1, Chase L. Beisel2,3, Jerome Bonnet4, Manish Kushwaha1 1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier JoVE 64236 Bioengineering