In JoVE (1)
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Articles by Chris A. Lindensmith in JoVE
Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM) Manuel Bedrossian1, Casey Barr2, Chris A. Lindensmith3, Kenneth Nealson2, Jay L. Nadeau1 1Department of Medical Engineering, California Institute of Technology, 2Department of Earth Sciences, University of Southern California, 3Jet Propulsion Laboratory, California Institute of Technology Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.