In JoVE (1)
Articles by Clémence Simon in JoVE
Visualisera Lignification Dynamics i anläggningar med Klicka på kemi: dubbel märkning är BLISS! Clémence Simon1, Corentin Spriet1, Simon Hawkins1, Cedric Lion1 1UGSF – Unité de Glycobiologie Structurale et Fonctionnelle, CNRS, UMR 8576, Université de Lille BLISS, en dubbel märkning protokoll för att studera lignification dynamik, utvecklades. Reportrar och en sekventiell kombination av SPAAC och CuAAC bioorthogonal klicka med syntetiska monolignol reaktioner, denna metod banar vägen till djupgående analys av de faktorer som reglerar biogenes av lignins i planta.
Other articles by Clémence Simon on PubMed
BLISS: A Bioorthogonal Dual-Labeling Strategy to Unravel Lignification Dynamics in Plants Cell Chemical Biology. | Pubmed ID: 28262560 A better in vivo understanding of lignin formation within plant cell walls will contribute to improving the valorization of plant-derived biomass. Although bioorthogonal chemistry provides a promising platform to study the lignification process, methodologies that simultaneously detect multiple chemical reporters in living organisms are still scarce. Here, we have developed an original bioorthogonal labeling imaging sequential strategy (BLISS) to visualize and analyze the incorporation of both p-hydroxyphenyl (H) and guaiacyl (G) units into lignin in vivo with a combination of strain-promoted and copper-catalyzed azide-alkyne cycloadditions. On our path to BLISS, we designed a new azide-tagged monolignol reporter for H units in metabolic lignin engineering and used it in conjunction with an alkyne-tagged G unit surrogate to study lignification dynamics in flax. Here, we show that BLISS provides precise spatial information on the zones of active lignification and reveals polarization in single-cell lignification dynamics.
BLISS: Shining a Light on Lignification in Plants Plant Signaling & Behavior. | Pubmed ID: 28786751 Lignin is a polyphenolic polymer of the plant cell wall formed by the oxidative polymerization of 3 main monomers called monolignols that give rise to the lignin H-, G- and S-units. Together with cellulose and hemicelluloses, lignin is a major component of plant biomass that is widely exploited by humans in numerous industrial processes. Despite recent advances in our understanding of monolignol biosynthesis, our current understanding of the spatio-temporal regulation of their transport and polymerization is more limited. In a recent publication, we have reported the development of an original Bioorthogonal Labeling Imaging Sequential Strategy (BLISS) that allows us to visualize the simultaneous incorporation dynamics of H and G monolignol reporters into lignifying cell walls of the flax stem. 11 Here, we extend the application of this strategy to other plant organs such as roots and rapidly discuss some of the contributions and perspectives of this new technique for improving our understanding of the lignification process in plants.