Other Publications (1)
Articles by Cynthia M. Chai in JoVE
Automated Analysis of a Nematode Population-based Chemosensory Preference Assay Cynthia M. Chai1,2, Christopher J. Cronin1,2, Paul W. Sternberg1,2 1Division of Biology and Bioengineering, California Institute of Technology, 2Howard Hughes Medical Institute, California Institute of Technology We present a behavior recording setup and protocol that enables automated analysis of the nematode, Caenorhabditis elegans' preference for soluble compounds in a population-based assay. This article describes the construction of a behavior chamber, the behavioral assay protocol, and video analysis software usage.
Other articles by Cynthia M. Chai on PubMed
CGAL, a Temperature-robust GAL4-UAS System for Caenorhabditis Elegans Nature Methods. Feb, 2017 | Pubmed ID: 27992408 The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.