In JoVE (3)
Other Publications (1)
Articles by Daniel Bode in JoVE
Intramikroskopi av Leukocytt-endothelial og blodplater-leukocytt Interaksjon i Mesenterial Vener i Mus Nadine Herr1, Maximilian Mauler1,2, Christoph Bode1, Daniel Duerschmied1 1Department of Cardiology and Angiology I, Heart Center, University of Freiburg, 2Faculty of Biology, University of Freiburg
Løse affinitetsrenset protein komplekser av Blå Native PAGE og Protein korrelasjon Profiling Mercedes Pardo1, Daniel Bode1, Lu Yu1, Jyoti S. Choudhary1 1Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute Her presenterer vi protokoller for affinitetsrensing av proteinkomplekser og deres separasjon ved blå nativ PAGE, etterfulgt av protein korrelasjon profilering ved hjelp av etiketten fri kvantitativ massespektrometri. Denne metoden er nyttig for å løse interactomes inn i forskjellige proteinkomplekser.
Digital PCR for kvantifisering sirkulerer MicroRNAs i akutt hjerteinfarkt og hjerte-og karsykdommer Louise Benning1, Samuel Robinson1,2, Marie Follo3, Lukas Andreas Heger1, Daniela Stallmann1, Daniel Duerschmied1, Christoph Bode1, Ingo Ahrens1,4, Marcus Hortmann1 1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne Sirkulerende microRNAs har vist lovende som biomarkers for kardiovaskulære sykdommer og akutt myokardisk infarkt. I denne studien beskriver vi en protokoll for miRNA utvinning, omvendt transkripsjon og digital PCR for den absolutte kvantifiseringen av miRNAs i serum av pasienter med hjerte-og karsykdommer.
Other articles by Daniel Bode on PubMed
Characterization of Two Distinct Nucleosome Remodeling and Deacetylase (NuRD) Complex Assemblies in Embryonic Stem Cells Molecular & Cellular Proteomics : MCP. Mar, 2016 | Pubmed ID: 26714524 Pluripotency and self-renewal, the defining properties of embryonic stem cells, are brought about by transcriptional programs involving an intricate network of transcription factors and chromatin remodeling complexes. The Nucleosome Remodeling and Deacetylase (NuRD) complex plays a crucial and dynamic role in the regulation of stemness and differentiation. Several NuRD-associated factors have been reported but how they are organized has not been investigated in detail. Here, we have combined affinity purification and blue native polyacrylamide gel electrophoresis followed by protein identification by mass spectrometry and protein correlation profiling to characterize the topology of the NuRD complex. Our data show that in mouse embryonic stem cells the NuRD complex is present as two distinct assemblies of differing topology with different binding partners. Cell cycle regulator Cdk2ap1 and transcription factor Sall4 associate only with the higher mass NuRD assembly. We further establish that only isoform Sall4a, and not Sall4b, associates with NuRD. By contrast, Suz12, a component of the PRC2 Polycomb repressor complex, associates with the lower mass entity. In addition, we identify and validate a novel NuRD-associated protein, Wdr5, a regulatory subunit of the MLL histone methyltransferase complex, which associates with both NuRD entities. Bioinformatic analyses of published target gene sets of these chromatin binding proteins are in agreement with these structural observations. In summary, this study provides an interesting insight into mechanistic aspects of NuRD function in stem cell biology. The relevance of our work has broader implications because of the ubiquitous nature of the NuRD complex. The strategy described here can be more broadly applicable to investigate the topology of the multiple complexes an individual protein can participate in.