Dirk Grimm Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty University of Heidelberg Biography Publications Institution JoVE Articles Dirk Grimm has not added a biography. If you are Dirk Grimm and would like to personalize this page please email our Author Liaison for assistance. Publications Determination of AAV Properties by Single Amino Acids: Go(o)d is in the Details Molecular Therapy. Methods & Clinical Development. Dec, 2022 | Pubmed ID: 36212908 Semirational Bioengineering of AAV Vectors with Increased Potency and Specificity for Systemic Gene Therapy of Muscle Disorders Science Advances. 09, 2022 | Pubmed ID: 36129972 In Vivo Adenine Base Editing Reverts C282Y and Improves Iron Metabolism in Hemochromatosis Mice Nature Communications. 09, 2022 | Pubmed ID: 36064805 Boosters for Adeno-associated Virus Vector (AAV) (r)evolution Cytotherapy. Aug, 2022 | Pubmed ID: 35999132 Fantastic AAV Gene Therapy Vectors and How to Find Them-Random Diversification, Rational Design and Machine Learning Pathogens (Basel, Switzerland). Jul, 2022 | Pubmed ID: 35890005 Intranasal Application of Adeno-associated Viruses: a Systematic Review Translational Research : the Journal of Laboratory and Clinical Medicine. Oct, 2022 | Pubmed ID: 35597541 Identification of Adeno-associated Virus Variants for Gene Transfer into Human Neural Cell Types by Parallel Capsid Screening Scientific Reports. 05, 2022 | Pubmed ID: 35589936 Right on Target: The Next Class of Efficient, Safe, and Specific RNAi Triggers Molecular Therapy. Nucleic Acids. Jun, 2022 | Pubmed ID: 35505965 Natural Killer Cells Act As an Extrinsic Barrier for in Vivo Reprogramming Development (Cambridge, England). 04, 2022 | Pubmed ID: 35420133 Ex vivo and In vivo Suppression of SARS-CoV-2 with Combinatorial AAV/RNAi Expression Vectors Molecular Therapy : the Journal of the American Society of Gene Therapy. 05, 2022 | Pubmed ID: 35038579 An RNA Interference/Adeno-Associated Virus Vector-Based Combinatorial Gene Therapy Approach Against Hepatitis E Virus Hepatology Communications. 04, 2022 | Pubmed ID: 34719133 Breaking the Sound Barrier: Towards Next-generation AAV Vectors for Gene Therapy of Hearing Disorders Hearing Research. 01, 2022 | Pubmed ID: 33268240 Cas9-specific Immune Responses Compromise Local and Systemic AAV CRISPR Therapy in Multiple Dystrophic Canine Models Nature Communications. 11, 2021 | Pubmed ID: 34819506 Adeno-Associated Viruses (AAV) and Host Immunity - A Race Between the Hare and the Hedgehog Frontiers in Immunology. 2021 | Pubmed ID: 34777364 Lentiviral and Adeno-associated Vectors Efficiently Transduce Mouse T Lymphocytes when Targeted to Murine CD8 Molecular Therapy. Methods & Clinical Development. Dec, 2021 | Pubmed ID: 34729380 Neutralizing Antibody Evasion and Transduction with Purified Extracellular Vesicle-Enveloped Adeno-Associated Virus Vectors Human Gene Therapy. 12, 2021 | Pubmed ID: 34445894 The Angiopoietin-Tie2 Pathway Regulates Purkinje Cell Dendritic Morphogenesis in a Cell-autonomous Manner Cell Reports. 08, 2021 | Pubmed ID: 34407407 Membrane-destabilizing Ionizable Phospholipids: Novel Components for Organ-selective MRNA Delivery and CRISPR-Cas Gene Editing Signal Transduction and Targeted Therapy. 05, 2021 | Pubmed ID: 34035211 A Versatile In Vivo System to Study Myc in Cell Reprogramming Methods in Molecular Biology (Clifton, N.J.). 2021 | Pubmed ID: 34019296 Best of Most Possible Worlds: Hybrid Gene Therapy Vectors Based on Parvoviruses and Heterologous Viruses Molecular Therapy : the Journal of the American Society of Gene Therapy. 12, 2021 | Pubmed ID: 33831556 Characterization of the GBoV1 Capsid and Its Antibody Interactions Viruses. 02, 2021 | Pubmed ID: 33672786 A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species Via Fluorescence-activated Cell Sorting Molecular Therapy. Methods & Clinical Development. Mar, 2021 | Pubmed ID: 33665228 'Hit and Run' Therapy Averts Macular Degeneration Nature Biomedical Engineering. 02, 2021 | Pubmed ID: 33580229 Optogenetic Control of Neisseria Meningitidis Cas9 Genome Editing Using an Engineered, Light-switchable Anti-CRISPR Protein Nucleic Acids Research. 03, 2021 | Pubmed ID: 33330940 Production, Processing, and Characterization of Synthetic AAV Gene Therapy Vectors Biotechnology Journal. Jan, 2021 | Pubmed ID: 32975881 Identification of a Myotropic AAV by Massively Parallel in Vivo Evaluation of Barcoded Capsid Variants Nature Communications. 10, 2020 | Pubmed ID: 33116134 Lab-Scale Production of Recombinant Adeno-Associated Viruses (AAV) for Expression of Optogenetic Elements Methods in Molecular Biology (Clifton, N.J.). 2020 | Pubmed ID: 32651911 Computational Design of Anti-CRISPR Proteins with Improved Inhibition Potency Nature Chemical Biology. 07, 2020 | Pubmed ID: 32284602 Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity Journal of Virology. 05, 2020 | Pubmed ID: 32213611 Pre-arrayed Pan-AAV Peptide Display Libraries for Rapid Single-Round Screening Molecular Therapy : the Journal of the American Society of Gene Therapy. 04, 2020 | Pubmed ID: 32105604 Ex Vivo/In vivo Gene Editing in Hepatocytes Using "All-in-One" CRISPR-Adeno-Associated Virus Vectors with a Self-Linearizing Repair Template IScience. Jan, 2020 | Pubmed ID: 31887661 Distinct Transduction of Muscle Tissue in Mice After Systemic Delivery of AAVpo1 Vectors Gene Therapy. 04, 2020 | Pubmed ID: 31624368 Severe Human Bocavirus 1 Respiratory Tract Infection in an Immunodeficient Child With Fatal Outcome The Pediatric Infectious Disease Journal. 09, 2019 | Pubmed ID: 31033910 Cell-specific CRISPR-Cas9 Activation by MicroRNA-dependent Expression of Anti-CRISPR Proteins Nucleic Acids Research. 07, 2019 | Pubmed ID: 30982889 Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 30912043 Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses Molecular Therapy. Methods & Clinical Development. Mar, 2019 | Pubmed ID: 30766894 A Robust and All-Inclusive Pipeline for Shuffling of Adeno-Associated Viruses ACS Synthetic Biology. 01, 2019 | Pubmed ID: 30513195 Impact of the Assembly-Activating Protein on Molecular Evolution of Synthetic Adeno-Associated Virus Capsids Human Gene Therapy. 01, 2019 | Pubmed ID: 29978729 Engineered Anti-CRISPR Proteins for Optogenetic Control of CRISPR-Cas9 Nature Methods. 11, 2018 | Pubmed ID: 30377362 AAVvector-mediated in Vivo Reprogramming into Pluripotency Nature Communications. 07, 2018 | Pubmed ID: 29985406 High-Throughput Dissection of AAV-Host Interactions: The Fast and the Curious Journal of Molecular Biology. 08, 2018 | Pubmed ID: 29782834 Split Cas9, Not Hairs - Advancing the Therapeutic Index of CRISPR Technology Biotechnology Journal. Sep, 2018 | Pubmed ID: 29316283 Regulation of Adult CNS Axonal Regeneration by the Post-transcriptional Regulator Cpeb1 Frontiers in Molecular Neuroscience. 2017 | Pubmed ID: 29379413 Small But Increasingly Mighty: Latest Advances in AAV Vector Research, Design, and Evolution Human Gene Therapy. 11, 2017 | Pubmed ID: 28835125 Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells Journal of Virology. 10, 2017 | Pubmed ID: 28768875 Next-generation AAV Vectors for Clinical Use: an Ever-accelerating Race Virus Genes. Oct, 2017 | Pubmed ID: 28762205 Plasmodium Berghei EXP-1 Interacts with Host Apolipoprotein H During Plasmodium Liver-stage Development Proceedings of the National Academy of Sciences of the United States of America. Feb, 2017 | Pubmed ID: 28137845 TALEN/CRISPR-mediated Engineering of a Promoterless Anti-viral RNAi Hairpin into an Endogenous MiRNA Locus Nucleic Acids Research. 01, 2017 | Pubmed ID: 27614072 Blocking Sense-strand Activity Improves Potency, Safety and Specificity of Anti-hepatitis B Virus Short Hairpin RNA EMBO Molecular Medicine. 09, 2016 | Pubmed ID: 27473329 Death Receptor-based Enrichment of Cas9-expressing Cells BMC Biotechnology. Feb, 2016 | Pubmed ID: 26883910 Zinc Finger Nuclease-based Double-strand Breaks Attenuate Malaria Parasites and Reveal Rare Microhomology-mediated End Joining Genome Biology. Nov, 2015 | Pubmed ID: 26573820 AAV8-mediated in Vivo Overexpression of MiR-155 Enhances the Protective Capacity of Genetically Attenuated Malarial Parasites Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2014 | Pubmed ID: 25189739 Ausdruck Determinanten Der Säugetier-Argonaute Proteine Bei Der Vermittlung Von Gen-silencing Nucleic Acids Research. Dec, 2011 | Pubmed ID: 22210886 Schicksal-Ablaufverfolgung Von Reifen Hepatozyten in Der Leber Maus Homöostase Und Regeneration The Journal of Clinical Investigation. Dec, 2011 | Pubmed ID: 22105172 Die Dosis Kann Das Gift Machen: Lehren Aus Der Negativen In-vivo Toxizitäten Verursacht Durch RNAi-Überexpression Silence. 2011 | Pubmed ID: 22029761 Zu Gehen Oder Nicht Zu Gehen, Das Ist Die Frage-, Sechs Persönliche Reflexionen über Wie Geographische Mobilität Ihre Karriere Und Das Leben Beeinträchtigen BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. Oct, 2011 | Pubmed ID: 21858845 Ausführung Der Mobilfunknetze Außer Kontrolle: Globale Dysregulation Der RNAi-Maschine in Der Menschlichen Pathologie Und Therapie Progress in Molecular Biology and Translational Science. 2011 | Pubmed ID: 21846572 Zelluläre RNA-Interferenz-Mechanismen. Vorwort Progress in Molecular Biology and Translational Science. 2011 | Pubmed ID: 21846566 Thermodynamische Stabilität Des Kleinen Haarnadel RNAs Beeinflusst Stark Den Ladevorgang Von Verschiedenen Säugetieren Argonautes Proceedings of the National Academy of Sciences of the United States of America. May, 2011 | Pubmed ID: 21576459 Argonaut-Proteine Sind Wichtige Determinanten Der RNAi Wirksamkeit, Toxizität Und Persistenz in Der Adulten Maus-Leber The Journal of Clinical Investigation. Sep, 2010 | Pubmed ID: 20697157 Sechs RNA-Viren Und Einundvierzig Hosts: Virale Kleine RNAs Und Modulation Der Kleine RNA-Repertoire in Vertebrate Und Wirbellosen-Systemen PLoS Pathogens. Feb, 2010 | Pubmed ID: 20169186 Low-Level ShRNA Zytotoxizität Kann MYC-induzierte Hepatozelluläres Karzinom in Erwachsenen Mäusen Beitragen Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2010 | Pubmed ID: 19844192 Kleine Unterdrückender RNAs: Staat-of-the-Art Advanced Drug Delivery Reviews. Jul, 2009 | Pubmed ID: 19427885 Ausdruck Der ShRNA Aus Gewebe-spezifischen Pol II Förderer Ist Eine Wirksame Und Sichere RNAi Therapeutische Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2008 | Pubmed ID: 18665161 Unterdrückender Hepatische Fettsäure-Transporter-Protein 5 In-vivo Kehrt Diät Induziert Alkoholfreie Fettsäuren Lebererkrankung Und Verbessert Die Hyperglykämie The Journal of Biological Chemistry. Aug, 2008 | Pubmed ID: 18524776 In Vitro Und In-vivo-gen Therapie Vektor Entwicklung über Multispecies Vermischung Und Neuausrichtung Der Adeno-assoziierte Viren Journal of Virology. Jun, 2008 | Pubmed ID: 18400866 Hepatische Lungenparenchym Ersatz in Den Mäusen Von Transplantierten Allogene Hepatozyten Ist Durch Eine Knochenmarktransplantation Erleichtert Und Von CD4-Zellen Vermittelt Hepatology (Baltimore, Md.). Feb, 2008 | Pubmed ID: 18220289 Therapeutische Anwendung Von RNAi: MRNA Gezielt Zur Hauptsendezeit Endlich Bereit Ist? The Journal of Clinical Investigation. Dec, 2007 | Pubmed ID: 18060021 RNAi Und Gentherapie: Eine Gegenseitige Anziehung Hematology / the Education Program of the American Society of Hematology. American Society of Hematology. Education Program. 2007 | Pubmed ID: 18024667 Schnelle Und Stabile Niederschlag Einer Endogenen Gens in Retinalen Pigmentepithels Human Gene Therapy. Oct, 2007 | Pubmed ID: 17892416 Kombinatorische RNAi: Eine Gewinnstrategie Für Den Wettlauf Gegen Die Sich Entwickelnden Ziele? Molecular Therapy : the Journal of the American Society of Gene Therapy. May, 2007 | Pubmed ID: 17311009 Ein Zwei-Mischling Bildschirm Identifiziert Cathepsins B Und L Als Uncoating Faktoren Für Adeno-assoziierte Virus 2 Und 8 Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2007 | Pubmed ID: 17235311 Der 37/67-Kilodalton Laminin-Rezeptor Ist Ein Rezeptor Für Adeno-assoziierte Virus Serotypen 8, 2, 3 Und 9 Journal of Virology. Oct, 2006 | Pubmed ID: 16973587 Todesfall in Den Mäusen Durch Übersättigung Der Zelluläre MicroRNA Bzw. Short Hairpin RNA-Bahnen Nature. May, 2006 | Pubmed ID: 16724069 Verbesserte Kardialen Gentransfer Von Transkriptionelle Und Transductional Ausrichtung Der Adeno-assoziierter Viraler Vektoren Cardiovascular Research. Apr, 2006 | Pubmed ID: 16448634 Leber Transduktion Mit Rekombinanten Adeno-assoziierte Virus Beschränkt Sich in Erster Linie Durch Kapsid Vektor Serotyp Nicht Genotyp Journal of Virology. Jan, 2006 | Pubmed ID: 16352567 Erhöhte Wartungs-und Persistenz Von Transgenen Durch Exzision Von Expressionskassetten Von Plasmid-Sequenzen in Vivo Human Gene Therapy. May, 2005 | Pubmed ID: 15916481 Adeno-assoziierte Virus-Vektoren Für Kurze Haarnadel-RNA-Expression Methods in Enzymology. 2005 | Pubmed ID: 15644194 Präklinische In-vivo-Evaluierung Von Pseudotypisierten Adeno-assoziierten Virus-Vektoren Für Die Gentherapie Leber Blood. Oct, 2003 | Pubmed ID: 12791653 Hilfsvirus, Optisch Steuerbare, Und Zwei-Plasmid Basierender Herstellung Von Adeno-assoziierten Virus-Vektoren Der Serotypen 1 Bis 6 Ist Molecular Therapy : the Journal of the American Society of Gene Therapy. Jun, 2003 | Pubmed ID: 12788658 Verfahren Zur Herstellung Von Gentransfervektoren Auf Adeno-assoziierten Virus Serotypen Methods (San Diego, Calif.). Oct, 2002 | Pubmed ID: 12413413 Primäre Humane Zellen Unterscheiden Sich in Ihrer Empfindlichkeit Gegenüber RAAV-2-vermittelten Gentransfer Und Die Dauer Der Expression Des Reportergens Journal of Virological Methods. Sep, 2002 | Pubmed ID: 12270659 Isolierung von Gentherapievektoren der nächsten Generation durch Engineering, Barcoding und Screening von Kapsidvarianten des Adeno-assoziierten Virus (AAV) Kleopatra Rapti1,2, Olena Maiakovska1,2, Jonas Becker1,2, Joanna Szumska1,2, Margarita Zayas1,2, Felix Bubeck1,2, Jixin Liu1,2, Emma Gerstmann1,2, Chiara Krämer1,2, Ellen Wiedtke1,2, Dirk Grimm1,2,3,4,5 1Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, 2BioQuant Center, BQ0030, University of Heidelberg, 3Cluster of Excellence CellNetworks, 4German Center for Infection Research (DZIF), 5German Center for Cardiovascular Research (DZHK) JoVE 64389 Bioengineering Engineering and Evolution of Synthetic Adeno-assoziierte Virus (AAV) Vektoren für die Gentherapie über DNA-Familie Shuffling Eike Kienle*1, Elena Senís*1, Kathleen Börner2, Dominik Niopek1, Ellen Wiedtke1, Stefanie Grosse1, Dirk Grimm1 1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University JoVE 3819 Immunology and Infection
Isolierung von Gentherapievektoren der nächsten Generation durch Engineering, Barcoding und Screening von Kapsidvarianten des Adeno-assoziierten Virus (AAV) Kleopatra Rapti1,2, Olena Maiakovska1,2, Jonas Becker1,2, Joanna Szumska1,2, Margarita Zayas1,2, Felix Bubeck1,2, Jixin Liu1,2, Emma Gerstmann1,2, Chiara Krämer1,2, Ellen Wiedtke1,2, Dirk Grimm1,2,3,4,5 1Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, 2BioQuant Center, BQ0030, University of Heidelberg, 3Cluster of Excellence CellNetworks, 4German Center for Infection Research (DZIF), 5German Center for Cardiovascular Research (DZHK) JoVE 64389 Bioengineering
Engineering and Evolution of Synthetic Adeno-assoziierte Virus (AAV) Vektoren für die Gentherapie über DNA-Familie Shuffling Eike Kienle*1, Elena Senís*1, Kathleen Börner2, Dominik Niopek1, Ellen Wiedtke1, Stefanie Grosse1, Dirk Grimm1 1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University JoVE 3819 Immunology and Infection
