Dominik Fiegle Institute of Cellular and Molecular Physiology Friedrich-Alexander University Erlangen-Nürnberg Biography Publications Institution JoVE Articles Dominik Fiegle At the University of Regensburg I received a thorough basic scientific knowledge in biology bachelor and master study.First I was trained in basic molecular biology in the plant physiology department. I applied and refined the acquired knowledge during practical courses in the master study. In the master's thesis in the department of virology and clinical microbiology I generated and validated a modified human cell line with furin protease over expression for the efficient recombinant production of native-like HIV envelope protein. To further expand my scientific and language skills I spend one semester at Charles University of Prague in the laboratory, working on the infection cycle of polyomavirus. As I gained insight into microscopic imaging during the time in Prague I decided to deepen my imaging skills during my PhD time. In addition, I wanted to transfer the acquired methods to a new research field and hence I joined the group of Prof. Volk in cardiac physiology at the Friedrich-Alexander University of Erlangen-Nürnberg as doctoral candidate. Here, we investigate the regulation of the t-tubular system (t-system) in animal and human cardiac myocytes. We use electrophysiology, confocal imaging, molecular biology and innovative in-vitro techniques to describe and investigate mechanisms of pathological remodelling of the t-system occurring in failing human hearts. Publications Isolamento de Cardiomiócitos Ventriculares Humanos de fatias de miocárdio cortadas por vibratome Dominik J. Fiegle1, Tilmann Volk1,2, Thomas Seidel1,2 1Institute of Cellular and Molecular Physiology, Friedrich-Alexander University Erlangen-Nürnberg, 2Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg JoVE 61167 Biology
Isolamento de Cardiomiócitos Ventriculares Humanos de fatias de miocárdio cortadas por vibratome Dominik J. Fiegle1, Tilmann Volk1,2, Thomas Seidel1,2 1Institute of Cellular and Molecular Physiology, Friedrich-Alexander University Erlangen-Nürnberg, 2Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg JoVE 61167 Biology