Articles by Eli S. J. Thoré in JoVE
Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri Charlotte Philippe1,2, Arnout F. Gregoir1, Eli S. J. Thoré1, Gudrun De Boeck2, Luc Brendonck1,3, Tom Pinceel1,4 1Animal Ecology, Global Change and Sustainable Development, University of Leuven, 2Systemic Physiological and Ecotoxicological Research, University of Antwerp, 3Water Research Group, Unit for Environmental Sciences and Management, North-West University, 4Centre for Environmental Management, University of the Free State In this work, we describe an acute, chronic and multigenerational bioassay to study the effects of single and combined stressors on the Turquoise killifish Nothobranchius furzeri. This protocol is designed to study life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.
Other articles by Eli S. J. Thoré on PubMed
Acute Sensitivity of the Killifish Nothobranchius Furzeri to a Combination of Temperature and Reference Toxicants (cadmium, Chlorpyrifos and 3,4-dichloroaniline) Environmental Science and Pollution Research International. | Pubmed ID: 29380199 Aquatic organisms of inland waters are often subjected to a combination of stressors. Yet, few experiments assess mixed stress effects beyond a select group of standard model organisms. We studied the joint toxicity of reference toxicants and increased temperature on the turquoise killifish, Nothobranchius furzeri, a promising model for ecotoxicological research due to the species' short life cycle and the production of drought-resistant eggs. The acute sensitivity of the larval stage (2dph) to three compounds (cadmium, 3,4-dichloroaniline and chlorpyrifos) was tested in combination with a temperature increase of 4 °C, mimicking global warming. Dose-response relationships were used to calculate 96h-LC of 0.28 mg/L (24 °C) and 0.39 mg/L (28 °C) for cadmium, 96h-LC of 9.75 mg/L (24 °C) and 6.61 mg/L (28 °C) for 3,4-dichloroaniline and 96h-LC of 15.4 μg/L (24 °C) and 14.2 μg/L (28 °C) for chlorpyrifos. After 24 h of exposure, the toxicity of all tested compounds was exacerbated under increased temperature. Furthermore, the interaction effect of cadmium and temperature could be predicted by the stress addition model (SAM). This suggests the applicability of the model for fish and at the same time indicates that the model could be suitable to predict effects of temperature-toxicant interactions.