Other Publications (1)
Articles by Elvira Hukasova in JoVE
Overvåking Kinase og fosfatase Aktiviteter Gjennom Cell Cycle av Ratiometric FRET Elvira Hukasova1, Helena Silva Cascales1, Shravan R. Kumar1, Arne Lindqvist1 1Department of Cell and Molecular Biology, Karolinska Institutet FRET-baserte journalister blir stadig mer brukt til å overvåke kinase og fosfatase aktiviteter i levende celler. Her beskriver vi en metode om hvordan du bruker FRET-baserte journalister å vurdere celle syklus-avhengige endringer i mål fosforylering.
Other articles by Elvira Hukasova on PubMed
Functional Characterization of the Pleckstrin Homology Domain of a Cellulose Synthase from the Oomycete Saprolegnia Monoica Biochemical and Biophysical Research Communications. Jan, 2012 | Pubmed ID: 22226909 Some oomycetes, for instance Saprolegnia parasitica, are severe fish pathogens that cause important economic losses worldwide. Cellulose biosynthesis is a vital process for this class of microorganisms, but the corresponding molecular mechanisms are poorly understood. Of all cellulose synthesizing enzymes known, only some oomycete cellulose synthases contain a pleckstrin homology (PH) domain. Some human PH domains bind specifically to phosphoinositides, but most PH domains bind phospholipids in a non-specific manner. In addition, some PH domains interact with various proteins. Here we have investigated the function of the PH domain of cellulose synthase 2 from the oomycete Saprolegnia monoica (SmCesA2), a species closely related to S. parasitica. The SmCesA2 PH domain is similar to the C-terminal PH domain of the human protein TAPP1. It binds in vitro to phosphoinositides, F-actin and microtubules, and co-localizes with F-actin in vivo. Our results suggest a role of the SmCesA2 PH domain in the regulation, trafficking and/or targeting of the cell wall synthesizing enzyme.