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Articles by En Cai in JoVE
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Imagens de fluorescência com um nanômetros Precisão (Fiona)
Yong Wang*1,2, En Cai*1,2, Janet Sheung1,2, Sang Hak Lee1,2, Kai Wen Teng2,3, Paul R. Selvin1,2,3
1Department of Physics, University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, 3Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign
Fluorophores individuais podem ser localizados com precisão nanométrica usando FIONA. Aqui um resumo da técnica FIONA é relatado, e como a realização de experimentos Fiona é descrito.
Other articles by En Cai on PubMed
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Using Fixed Fiduciary Markers for Stage Drift Correction
Optics Express.
May, 2012 |
Pubmed ID: 22714205 To measure nanometric features with super-resolution requires that the stage, which holds the sample, be stable to nanometric precision. Herein we introduce a new method that uses conventional equipment, is low cost, and does not require intensive computation. Fiduciary markers of approximately 1 µm x 1 µm x 1 µm in x, y, and z dimensions are placed at regular intervals on the coverslip. These fiduciary markers are easy to put down, are completely stationary with respect to the coverslip, are bio-compatible, and do not interfere with fluorescence or intensity measurements. As the coverslip undergoes drift (or is purposely moved), the x-y center of the fiduciary markers can be readily tracked to 1 nanometer using a Gaussian fit. By focusing the light slightly out-of-focus, the z-axis can also be tracked to < 5 nm for dry samples and
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