Other Publications (1)
Articles by Eric Birkenhauer in JoVE
Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy Eric Birkenhauer1, Suresh Neethirajan1 1BioNano Laboratory, School of Engineering, University of Guelph Here, we present a protocol explaining the use of Kelvin probe force microscopy as a tool for generating high resolution nano-scale surface potential maps. This tool was applied to assess the role of surface potential on the binding capacity of microorganisms to substrate surfaces.
Other articles by Eric Birkenhauer on PubMed
Collagen and Hyaluronan at Wound Sites Influence Early Polymicrobial Biofilm Adhesive Events BMC Microbiology. 2014 | Pubmed ID: 25026865 Wounds can easily become chronically infected, leading to secondary health complications, which occur more frequently in individuals with diabetes, compromised immune systems, and those that have suffered severe burns. When wounds become chronically infected, biofilm producing microbes are often isolated from these sites. The presence of a biofilm at a wound site has significant negative impact on the treatment outcomes, as biofilms are characteristically recalcitrant to removal, in part due to the formation of a protective matrix that shield residents organisms from inimical forces. Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) are two of the organisms most prevalently isolated from wound sites, and are of particular concern due to their elevated levels of antibiotic resistance, rapid growth, and exotoxin production. In order to understand the biofilm forming abilities of these microbes in a simulated wound environment we used a microtiter plate assay to assess the ability of these two organisms to bind to proteins that are typically found at wound sites: collagen and hyaluronan.