Articles by Fedir N. Kiskin in JoVE
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood Mark L. Ormiston1, Mark R. Toshner2, Fedir N. Kiskin1, Christopher J. Z. Huang1, Emily Groves1, Nicholas W. Morrell1, Amer A. Rana1 1Department of Medicine, University of Cambridge, 2Papworth Hospital This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.
Other articles by Fedir N. Kiskin on PubMed
Structural Organization of Weibel-Palade Bodies Revealed by Cryo-EM of Vitrified Endothelial Cells Proceedings of the National Academy of Sciences of the United States of America. Oct, 2009 | Pubmed ID: 19805028 In endothelial cells, the multifunctional blood glycoprotein von Willebrand Factor (VWF) is stored for rapid exocytic release in specialized secretory granules called Weibel-Palade bodies (WPBs). Electron cryomicroscopy at the thin periphery of whole, vitrified human umbilical vein endothelial cells (HUVECs) is used to directly image WPBs and their interaction with a 3D network of closely apposed membranous organelles, membrane tubules, and filaments. Fourier analysis of images and tomographic reconstruction show that VWF is packaged as a helix in WPBs. The helical signature of VWF tubules is used to identify VWF-containing organelles and characterize their paracrystalline order in low dose images. We build a 3D model of a WPB in which individual VWF helices can bend, but in which the paracrystalline packing of VWF tubules, closely wrapped by the WPB membrane, is associated with the rod-like morphology of the granules.