In JoVE (1)
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Articles by Fettah Erdogan in JoVE
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation Fettah Erdogan1, Cristina Lento1, Ayat Yaseen1, Roksana Nowroozi-Dayeni1, Sasha Kheyson1, Gerald F. Audette1,2 1Department of Chemistry, York University, 2The Centre for Research on Biomolecular Interactions, York University Here, we present a protocol to knockout a gene of interest involved in plasmid conjugation and subsequently analyze the impact of its absence using mating assays. The function of the gene is further explored to a specific region of its sequence using deletion or point mutations.