In JoVE (1)
Other Publications (0)
Articles by Frederik Rudolph in JoVE
Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography Matthew C. Johnson*1, Frederik Rudolph*1,2, Tina M. Dreaden*3, Gengxiang Zhao*1, Bridgette A. Barry3, Ingeborg Schmidt-Krey1,3 1School of Biology, Georgia Institute of Technology, 2Department of Molecular Pharmacology, RWTH Aachen University, 3School of Chemistry and Biochemistry, Georgia Institute of Technology Evaluating two-dimensional (2D) crystallization trials for the formation of ordered membrane protein arrays is a highly critical and difficult task in electron crystallography. Here we describe our approach in screening for and identifying 2D crystals of predominantly small membrane proteins in the range of 15 – 90kDa.