In JoVE (1)

Other Publications (2)

Articles by Heather N. Turner in JoVE

Other articles by Heather N. Turner on PubMed

Macrophages Gain a Partner at the Table: Epidermal Cells Digest Peripheral Dendritic Debris in Drosophila

Neuron. Feb, 2014  |  Pubmed ID: 24507184

In this issue of Neuron, Han et al. (2014) develop powerful methods to visualize phagocytosis of Drosophila peripheral sensory neuron dendrites. Remarkably, epidermal cells rather than professional phagocytes are the primary mediators of debris clearance, using both familiar and new molecular players.

The TRP Channels Pkd2, NompC, and Trpm Act in Cold-Sensing Neurons to Mediate Unique Aversive Behaviors to Noxious Cold in Drosophila

Current Biology : CB. Dec, 2016  |  Pubmed ID: 27818173

The basic mechanisms underlying noxious cold perception are not well understood. We developed Drosophila assays for noxious cold responses. Larvae respond to near-freezing temperatures via a mutually exclusive set of singular behaviors-in particular, a full-body contraction (CT). Class III (CIII) multidendritic sensory neurons are specifically activated by cold and optogenetic activation of these neurons elicits CT. Blocking synaptic transmission in CIII neurons inhibits CT. Genetically, the transient receptor potential (TRP) channels Trpm, NompC, and Polycystic kidney disease 2 (Pkd2) are expressed in CIII neurons, where each is required for CT. Misexpression of Pkd2 is sufficient to confer cold responsiveness. The optogenetic activation level of multimodal CIII neurons determines behavioral output, and visualization of neuronal activity supports this conclusion. Coactivation of cold- and heat-responsive sensory neurons suggests that the cold-evoked response circuitry is dominant. Our Drosophila model will enable a sophisticated molecular genetic dissection of cold nociceptive genes and circuits.

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