Hongping Wei Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology Chinese Academy of Sciences Biography Publications Institution JoVE Articles Hongping Wei has not added a biography. If you are Hongping Wei and would like to personalize this page please email our Author Liaison for assistance. Publications Linear Epitope Landscape of the SARS-CoV-2 Spike Protein Constructed from 1,051 COVID-19 Patients Cell Reports. Mar, 2021 | Pubmed ID: 33761319 A Novel Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes Frontiers in Cellular and Infection Microbiology. 2021 | Pubmed ID: 33738267 Internal Cell-penetrating Peptide-mediated Internalization Enables a Chimeric Lysin to Target Intracellular Pathogens International Journal of Pharmaceutics. Mar, 2021 | Pubmed ID: 33711472 Rapid Genome-wide Sequence Typing of African Swine Fever Virus Based on Alleles Virus Research. Mar, 2021 | Pubmed ID: 33667625 Genomic Monitoring of SARS-CoV-2 Uncovers an Nsp1 Deletion Variant That Modulates Type I Interferon Response Cell Host & Microbe. 03, 2021 | Pubmed ID: 33548198 Developing Multiplex DdPCR Assays for SARS-CoV-2 Detection Based on Probe Mix and Amplitude Based Multiplexing Expert Review of Molecular Diagnostics. 01, 2021 | Pubmed ID: 33380245 A Choline-Recognizing Monomeric Lysin, ClyJ-3m, Shows Elevated Activity Against Streptococcus Pneumoniae Antimicrobial Agents and Chemotherapy. 11, 2020 | Pubmed ID: 32958710 Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Microorganisms. May, 2020 | Pubmed ID: 32397601 Application of Adaptive Evolution to Improve the Stability of Bacteriophages During Storage Viruses. 04, 2020 | Pubmed ID: 32283683 Construction and Characterization of a Chimeric Lysin ClyV with Improved Bactericidal Activity Against Streptococcus Agalactiae in Vitro and in Vivo Applied Microbiology and Biotechnology. Feb, 2020 | Pubmed ID: 31900556 Linker Editing of Pneumococcal Lysin ClyJ Conveys Improved Bactericidal Activity Antimicrobial Agents and Chemotherapy. 01, 2020 | Pubmed ID: 31767724 Nanopore Sequencing of African Swine Fever Virus Science China. Life Sciences. 01, 2020 | Pubmed ID: 31701406 Arboviruses in the East African Community Partner States: a Review of Medically Important Mosquito-borne Arboviruses Pathogens and Global Health. 07, 2019 | Pubmed ID: 31664886 Droplet Digital PCR Applications in the Tuberculosis World Tuberculosis (Edinburgh, Scotland). 07, 2019 | Pubmed ID: 31378274 A Novel Double Core-Shell Bionanoparticle Formed by Ferritin and Baculoviral Polyhedrin Journal of Nanoscience and Nanotechnology. Nov, 2019 | Pubmed ID: 31039919 Construction of Lentivirus-Based Reference Material for RT-PCR Detection of Middle East Respiratory Syndrome Coronavirus and Its Application in External Quality Assessment Journal of Nanoscience and Nanotechnology. Sep, 2019 | Pubmed ID: 30961704 Bacteriophages Isolated in China for the Control of Pectobacterium Carotovorum Causing Potato Soft Rot in Kenya Virologica Sinica. Jun, 2019 | Pubmed ID: 30868359 ClyJ Is a Novel Pneumococcal Chimeric Lysin with a Cysteine- and Histidine-Dependent Amidohydrolase/Peptidase Catalytic Domain Antimicrobial Agents and Chemotherapy. 04, 2019 | Pubmed ID: 30642930 Biocontrol of Phytobacteria with Bacteriophage Cocktails Pest Management Science. Jul, 2019 | Pubmed ID: 30624034 Exploring a Phage-based Real-time PCR Assay for Diagnosing Acinetobacter Baumannii Bloodstream Infections with High Sensitivity Analytica Chimica Acta. Dec, 2018 | Pubmed ID: 30442396 Virulence Attenuation and Immune Clearance Mediated by a Phage Lysin-derived Protein The EMBO Journal. 09, 2018 | Pubmed ID: 30037823 Activity of the Chimeric Lysin ClyR Against Common Gram-Positive Oral Microbes and Its Anticaries Efficacy in Rat Models Viruses. 07, 2018 | Pubmed ID: 30036941 Effects of a Chimeric Lysin Against Planktonic and Sessile Hint at Potential Application in Endodontic Therapy Viruses. 05, 2018 | Pubmed ID: 29844267 Parallel Susceptibility Testing of Bacteria Through Culture-quantitative PCR in 96-well Plates International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases. May, 2018 | Pubmed ID: 29559368 Detection, Isolation, and Characterization of Chikungunya Viruses Associated with the Pakistan Outbreak of 2016-2017 Virologica Sinica. Dec, 2017 | Pubmed ID: 29285673 Extensive Evolution Analysis of the Global Chikungunya Virus Strains Revealed the Origination of CHIKV Epidemics in Pakistan in 2016 Virologica Sinica. Dec, 2017 | Pubmed ID: 29243070 Developing a Bacteriophage Cocktail for Biocontrol of Potato Bacterial Wilt Virologica Sinica. Dec, 2017 | Pubmed ID: 29168148 Accurate Detection of Methicillin-Resistant Staphylococcus Aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR Journal of Clinical Microbiology. 10, 2017 | Pubmed ID: 28724560 Rapid and Selective Detection of E. Coli O157:H7 Combining Phagomagnetic Separation with Enzymatic Colorimetry Food Chemistry. Nov, 2017 | Pubmed ID: 28551244 Combining Phagomagnetic Separation with Immunoassay for Specific, Fast and Sensitive Detection of Staphylococcus Aureus Talanta. Aug, 2017 | Pubmed ID: 28501172 A Novel Chimeric Lysin with Robust Antibacterial Activity Against Planktonic and Biofilm Methicillin-resistant Staphylococcus Aureus Scientific Reports. 01, 2017 | Pubmed ID: 28067286 Rapid Detection of Viral Antibodies Based on Multifunctional Staphylococcus Aureus Nanobioprobes Enzyme and Microbial Technology. Dec, 2016 | Pubmed ID: 27866631 Antibiofilm Activities of a Novel Chimeolysin Against Streptococcus Mutans Under Physiological and Cariogenic Conditions Antimicrobial Agents and Chemotherapy. 12, 2016 | Pubmed ID: 27736755 Rapid and Sensitive Detection of HIV-1 P24 Antigen by Immunomagnetic Separation Coupled with Catalytic Fluorescent Immunoassay Analytical and Bioanalytical Chemistry. Sep, 2016 | Pubmed ID: 27351993 Comparison Between a Chimeric Lysin ClyH and Other Enzymes for Extracting DNA to Detect Methicillin Resistant Staphylococcus Aureus by Quantitative PCR World Journal of Microbiology & Biotechnology. Jan, 2016 | Pubmed ID: 26596268 Sensitive and Rapid Detection of Staphylococcus Aureus in Milk Via Cell Binding Domain of Lysin Biosensors & Bioelectronics. Mar, 2016 | Pubmed ID: 26433070 Antibacterial Activity of a Novel Peptide-Modified Lysin Against Acinetobacter Baumannii and Pseudomonas Aeruginosa Frontiers in Microbiology. 2015 | Pubmed ID: 26733995 A Chimeolysin with Extended-spectrum Streptococcal Host Range Found by an Induced Lysis-based Rapid Screening Method Scientific Reports. Nov, 2015 | Pubmed ID: 26607832 Study of the Interactions Between Endolysin and Bacterial Peptidoglycan on S. Aureus by Dynamic Force Spectroscopy Nanoscale. Oct, 2015 | Pubmed ID: 26324763 Simple and Rapid Preparation of Red Fluorescence and Red Color S. Aureus Derived Nanobioparticles for Pathogen Detection Journal of Microbiological Methods. Aug, 2015 | Pubmed ID: 26003440 Aspartate Inhibits Staphylococcus Aureus Biofilm Formation FEMS Microbiology Letters. Apr, 2015 | Pubmed ID: 25687923 Molecular Dissection of Phage Lysin PlySs2: Integrity of the Catalytic and Cell Wall Binding Domains is Essential for Its Broad Lytic Activity Virologica Sinica. Feb, 2015 | Pubmed ID: 25680444 Bacteriophages, Revitalized After 100 Years in the Shadow of Antibiotics Virologica Sinica. Feb, 2015 | Pubmed ID: 25680442 Isolation and Complete Genome Sequence of a Novel Virulent Mycobacteriophage, CASbig Virologica Sinica. Feb, 2015 | Pubmed ID: 25646930 Anti-mycobacterial Peptides: from Human to Phage Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. 2015 | Pubmed ID: 25613372 Construction of a Chimeric Lysin Ply187N-V12C with Extended Lytic Activity Against Staphylococci and Streptococci Microbial Biotechnology. Mar, 2015 | Pubmed ID: 25219798 Engineered Bacteriophage Lysins As Novel Anti-infectives Frontiers in Microbiology. 2014 | Pubmed ID: 25360133 In Vivo Imaging of Protein-protein and RNA-protein Interactions Using Novel Far-red Fluorescence Complementation Systems Nucleic Acids Research. Jul, 2014 | Pubmed ID: 24813442 Degradation of Methicillin-resistant Staphylococcus Aureus Biofilms Using a Chimeric Lysin Biofouling. 2014 | Pubmed ID: 24742201 Rapid Detection of New Delhi Metallo-β-lactamase Gene and Variants Coding for Carbapenemases with Different Activities by Use of a PCR-based in Vitro Protein Expression Method Journal of Clinical Microbiology. Jun, 2014 | Pubmed ID: 24671780 PCR-based in Vitro Synthesis of Hepatitis C Virus NS3 Protease for Rapid Phenotypic Resistance Testing of Protease Inhibitors Journal of Clinical Microbiology. Apr, 2014 | Pubmed ID: 24452171 Rapid Detection of Mycobacterium Tuberculosis and Pyrazinamide Susceptibility Related to PncA Mutations in Sputum Specimens Through an Integrated Gene-to-protein Function Approach Journal of Clinical Microbiology. Jan, 2014 | Pubmed ID: 24226918 Novel Chimeric Lysin with High-level Antimicrobial Activity Against Methicillin-resistant Staphylococcus Aureus in Vitro and in Vivo Antimicrobial Agents and Chemotherapy. 2014 | Pubmed ID: 24189265 Imaging of MRNA-protein Interactions in Live Cells Using Novel MCherry Trimolecular Fluorescence Complementation Systems PloS One. 2013 | Pubmed ID: 24260494 Use of HGluc/tdTomato Pair for Sensitive BRET Sensing of Protease with High Solution Media Tolerance Talanta. May, 2013 | Pubmed ID: 23618151 Reversibly Acetylated Lysine Residues Play Important Roles in the Enzymatic Activity of Escherichia Coli N-hydroxyarylamine O-acetyltransferase The FEBS Journal. May, 2013 | Pubmed ID: 23452042 Effects of Fullerene Derivatives on Bioluminescence and Application for Protease Detection Chemical Communications (Cambridge, England). Nov, 2012 | Pubmed ID: 23037447 Rapid Detection of Filoviruses by Real-time TaqMan Polymerase Chain Reaction Assays Virologica Sinica. Oct, 2012 | Pubmed ID: 23001480 Existence of Separate Domains in Lysin PlyG for Recognizing Bacillus Anthracis Spores and Vegetative Cells Antimicrobial Agents and Chemotherapy. Oct, 2012 | Pubmed ID: 22802245 Buffer Enhanced Bioluminescence Resonance Energy Transfer Sensor Based on Gaussia Luciferase for in Vitro Detection of Protease Analytica Chimica Acta. Apr, 2012 | Pubmed ID: 22483217 Human Cytomegalovirus UL94 is a Nucleocytoplasmic Shuttling Protein Containing Two NLSs and One NES Virus Research. Jun, 2012 | Pubmed ID: 22414299 Rapid Colorimetric Testing for Pyrazinamide Susceptibility of M. Tuberculosis by a PCR-based In-vitro Synthesized Pyrazinamidase Method PloS One. 2011 | Pubmed ID: 22102918 Encapsulation of Gold Nanoparticles by Simian Virus 40 Capsids Nanoscale. Oct, 2011 | Pubmed ID: 21879117 Aptamer Beacons for Visualization of Endogenous Protein HIV-1 Reverse Transcriptase in Living Cells Biosensors & Bioelectronics. Oct, 2011 | Pubmed ID: 21824761 Poliovirus 2A(pro) Induces the Nucleic Translocation of Poliovirus 3CD and 3C' Proteins Acta Biochimica Et Biophysica Sinica. Jan, 2011 | Pubmed ID: 21173057 Colorimetric Detection of Melamine in Complex Matrices Based on Cysteamine-modified Gold Nanoparticles The Analyst. Jan, 2011 | Pubmed ID: 20877886 Construction of Bifunctional Phage Display for Biological Analysis and Immunoassay Analytical Biochemistry. Jan, 2010 | Pubmed ID: 19699710 Cloning and Characterization of NAD-dependent Protein Deacetylase (Rv1151c) from Mycobacterium Tuberculosis Biochemistry. Biokhimiia. Jul, 2009 | Pubmed ID: 19747094 Visualization of the Dynamic Multimerization of Human Cytomegalovirus Pp65 in Punctuate Nuclear Foci Virology. Sep, 2009 | Pubmed ID: 19647850 Development of a Universal Phosphorylated Peptide-binding Protein for Simultaneous Assay of Kinases Biosensors & Bioelectronics. May, 2009 | Pubmed ID: 19349157 The Tegument Protein UL94 of Human Cytomegalovirus As a Binding Partner for Tegument Protein Pp28 Identified by Intracellular Imaging Virology. May, 2009 | Pubmed ID: 19345970 Reduction of Dopamine Level Enhances the Attractiveness of Male Drosophila to Other Males PloS One. 2009 | Pubmed ID: 19238209 Increased Dopamine Level Enhances Male-male Courtship in Drosophila The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. May, 2008 | Pubmed ID: 18495888 Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification Raphael Nyaruaba1,2,3, Xiaohong Li1, Caroline Mwaliko2,3,4, Changchang Li1, Matilu Mwau5, Nelson Odiwour1,2,3, Elishiba Muturi1,2,3, Caroline Muema1,2,3, Junhua Li1, Junping Yu1, Hongping Wei1 1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute JoVE 62295 Biology
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification Raphael Nyaruaba1,2,3, Xiaohong Li1, Caroline Mwaliko2,3,4, Changchang Li1, Matilu Mwau5, Nelson Odiwour1,2,3, Elishiba Muturi1,2,3, Caroline Muema1,2,3, Junhua Li1, Junping Yu1, Hongping Wei1 1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute JoVE 62295 Biology