Jürgen Plitzko Max Planck Institute of Biochemistry Biography Publications Institution JoVE Articles Jürgen Plitzko has not added a biography. If you are Jürgen Plitzko and would like to personalize this page please email our Author Liaison for assistance. Publications In Situ Cryo-electron Tomography Reveals Gradient Organization of Ribosome Biogenesis in Intact Nucleoli Nature Communications. 09, 2021 | Pubmed ID: 34508074 High-Yield Production, Characterization, and Functionalization of Recombinant Magnetosomes in the Synthetic Bacterium Rhodospirillum Rubrum "magneticum" Advanced Biology. 09, 2021 | Pubmed ID: 34296829 Molecular-scale Visualization of Sarcomere Contraction Within Native Cardiomyocytes Nature Communications. 07, 2021 | Pubmed ID: 34215727 Structural Basis for VIPP1 Oligomerization and Maintenance of Thylakoid Membrane Integrity Cell. Jul, 2021 | Pubmed ID: 34166613 Cryo-EM Structure of the Cetacean Morbillivirus Nucleoprotein-RNA Complex Journal of Structural Biology. 09, 2021 | Pubmed ID: 34089875 A Streamlined Workflow for Automated Cryo Focused Ion Beam Milling Journal of Structural Biology. 09, 2021 | Pubmed ID: 33971286 A Bacterial Cytolinker Couples Positioning of Magnetic Organelles to Cell Shape Control Proceedings of the National Academy of Sciences of the United States of America. 12, 2020 | Pubmed ID: 33257551 Preparing Samples from Whole Cells Using Focused-ion-beam Milling for Cryo-electron Tomography Nature Protocols. 06, 2020 | Pubmed ID: 32405053 Charting the Native Architecture of Thylakoid Membranes with Single-molecule Precision ELife. 04, 2020 | Pubmed ID: 32297859 Tripartite Phase Separation of Two Signal Effectors with Vesicles Priming B Cell Responsiveness Nature Communications. 02, 2020 | Pubmed ID: 32051419 Direct Visualization of Degradation Microcompartments at the ER Membrane Proceedings of the National Academy of Sciences of the United States of America. 01, 2020 | Pubmed ID: 31882451 In Situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography Scientific Reports. 12, 2019 | Pubmed ID: 31836773 Molecular Architecture of the SYCP3 Fibre and Its Interaction with DNA Open Biology. 10, 2019 | Pubmed ID: 31615332 A Gradient-forming MipZ Protein Mediating the Control of Cell Division in the Magnetotactic Bacterium Magnetospirillum gryphiswaldense Molecular Microbiology. 11, 2019 | Pubmed ID: 31419361 Cryo-EM Structure of the Native Rhodopsin Dimer in Nanodiscs The Journal of Biological Chemistry. 09, 2019 | Pubmed ID: 31399513 Liquid-crystalline Phase Transitions in Lipid Droplets Are Related to Cellular States and Specific Organelle Association Proceedings of the National Academy of Sciences of the United States of America. 08, 2019 | Pubmed ID: 31375636 A Cryo-FIB Lift-out Technique Enables Molecular-resolution Cryo-ET Within Native Caenorhabditis Elegans Tissue Nature Methods. 08, 2019 | Pubmed ID: 31363205 MamY is a Membrane-bound Protein That Aligns Magnetosomes and the Motility Axis of Helical Magnetotactic Bacteria Nature Microbiology. 11, 2019 | Pubmed ID: 31358981 Biogenic Regions of Cyanobacterial Thylakoids Form Contact Sites with the Plasma Membrane Nature Plants. 04, 2019 | Pubmed ID: 30962530 The Polar Organizing Protein PopZ Is Fundamental for Proper Cell Division and Segregation of Cellular Content in MBio. 03, 2019 | Pubmed ID: 30862753 Cryo-EM Structure of the Active, G-protein Complexed, Human CGRP Receptor Nature. 09, 2018 | Pubmed ID: 30209400 Structure of a Hibernating 100S Ribosome Reveals an Inactive Conformation of the Ribosomal Protein S1 Nature Microbiology. 10, 2018 | Pubmed ID: 30177741 Expanded Coverage of the 26S Proteasome Conformational Landscape Reveals Mechanisms of Peptidase Gating Cell Reports. 07, 2018 | Pubmed ID: 30067984 Connectivity of Centermost Chromatophores in Rhodobacter Sphaeroides Bacteria Molecular Microbiology. 09, 2018 | Pubmed ID: 29995992 Structure of the Adenosine-bound Human Adenosine A Receptor-G Complex Nature. 06, 2018 | Pubmed ID: 29925945 In Situ Architecture of the Algal Nuclear Pore Complex Nature Communications. 06, 2018 | Pubmed ID: 29915221 Bacterial Encapsulins As Orthogonal Compartments for Mammalian Cell Engineering Nature Communications. 05, 2018 | Pubmed ID: 29777103 Structural Studies of Tailed Spindle Virus Reveal a Structural Paradigm Used in the Assembly of Spindle-shaped Viruses Proceedings of the National Academy of Sciences of the United States of America. 02, 2018 | Pubmed ID: 29440399 In Vivo Coating of Bacterial Magnetic Nanoparticles by Magnetosome Expression of Spider Silk-Inspired Peptides Biomacromolecules. 03, 2018 | Pubmed ID: 29357230 The Dual Role of MamB in Magnetosome Membrane Assembly and Magnetite Biomineralization Molecular Microbiology. Feb, 2018 | Pubmed ID: 29243866 Proteasomes Tether to Two Distinct Sites at the Nuclear Pore Complex Proceedings of the National Academy of Sciences of the United States of America. 12, 2017 | Pubmed ID: 29229809 The Structure of the COPI Coat Determined Within the Cell ELife. 11, 2017 | Pubmed ID: 29148969 The Eukaryotic CO-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization Cell. Sep, 2017 | Pubmed ID: 28938114 Structural Biology Outside the Box-inside the Cell Current Opinion in Structural Biology. 10, 2017 | Pubmed ID: 28735108 In Situ Structural Studies of Tripeptidyl Peptidase II (TPPII) Reveal Spatial Association with Proteasomes Proceedings of the National Academy of Sciences of the United States of America. 04, 2017 | Pubmed ID: 28396430 Structures of the Cyanobacterial Circadian Oscillator Frozen in a Fully Assembled State Science (New York, N.Y.). 03, 2017 | Pubmed ID: 28302852 Dissecting the Molecular Organization of the Translocon-associated Protein Complex Nature Communications. 02, 2017 | Pubmed ID: 28218252 Structural Insights into the Functional Cycle of the ATPase Module of the 26S Proteasome Proceedings of the National Academy of Sciences of the United States of America. 02, 2017 | Pubmed ID: 28115689 Optimized Cryo-focused Ion Beam Sample Preparation Aimed at in Situ Structural Studies of Membrane Proteins Journal of Structural Biology. 02, 2017 | Pubmed ID: 27444390 Segregation of Prokaryotic Magnetosomes Organelles is Driven by Treadmilling of a Dynamic Actin-like MamK Filament BMC Biology. 10, 2016 | Pubmed ID: 27733152 Structure of the Human 26S Proteasome at a Resolution of 3.9 Å Proceedings of the National Academy of Sciences of the United States of America. 07, 2016 | Pubmed ID: 27342858 Genetic and Ultrastructural Analysis Reveals the Key Players and Initial Steps of Bacterial Magnetosome Membrane Biogenesis PLoS Genetics. 06, 2016 | Pubmed ID: 27286560 Overproduction of Magnetosomes by Genomic Amplification of Biosynthesis-Related Gene Clusters in a Magnetotactic Bacterium Applied and Environmental Microbiology. 05, 2016 | Pubmed ID: 26969709 Visualizing the Molecular Sociology at the HeLa Cell Nuclear Periphery Science (New York, N.Y.). Feb, 2016 | Pubmed ID: 26917770 Volta Phase Plate Cryo-EM of the Small Protein Complex Prx3 Nature Communications. Jan, 2016 | Pubmed ID: 26817416 Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy Biophysical Journal. Feb, 2016 | Pubmed ID: 26769364 Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography Bio-protocol. Sep, 2015 | Pubmed ID: 27294174 Structural Basis of Vesicle Formation at the Inner Nuclear Membrane Cell. Dec, 2015 | Pubmed ID: 26687357 In Situ Structural Analysis of Golgi Intracisternal Protein Arrays Proceedings of the National Academy of Sciences of the United States of America. Sep, 2015 | Pubmed ID: 26311849 A Focused Ion Beam Milling and Lift-out Approach for Site-specific Preparation of Frozen-hydrated Lamellas from Multicellular Organisms Journal of Structural Biology. Nov, 2015 | Pubmed ID: 26216184 Native Architecture of the Chlamydomonas Chloroplast Revealed by in Situ Cryo-electron Tomography ELife. Jan, 2015 | Pubmed ID: 25584625 Volta Potential Phase Plate for In-focus Phase Contrast Transmission Electron Microscopy Proceedings of the National Academy of Sciences of the United States of America. Nov, 2014 | Pubmed ID: 25331897 A Tailored GalK Counterselection System for Efficient Markerless Gene Deletion and Chromosomal Tagging in Magnetospirillum Gryphiswaldense Applied and Environmental Microbiology. Jul, 2014 | Pubmed ID: 24814778 Incomplete Pneumolysin Oligomers Form Membrane Pores Open Biology. Apr, 2014 | Pubmed ID: 24759615 Deep Classification of a Large Cryo-EM Dataset Defines the Conformational Landscape of the 26S Proteasome Proceedings of the National Academy of Sciences of the United States of America. Apr, 2014 | Pubmed ID: 24706844 Three-dimensional Architecture of Actin Filaments in Listeria Monocytogenes Comet Tails Proceedings of the National Academy of Sciences of the United States of America. Dec, 2013 | Pubmed ID: 24306931 Opening Windows into the Cell: Focused-ion-beam Milling for Cryo-electron Tomography Current Opinion in Structural Biology. Oct, 2013 | Pubmed ID: 24090931 Near-atomic Resolution Structural Model of the Yeast 26S Proteasome Proceedings of the National Academy of Sciences of the United States of America. Sep, 2012 | Pubmed ID: 22927375 Integrative Approaches for Cellular Cryo-electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining Methods in Cell Biology. 2012 | Pubmed ID: 22857933 Focused Ion Beam Micromachining of Eukaryotic Cells for Cryoelectron Tomography Proceedings of the National Academy of Sciences of the United States of America. Mar, 2012 | Pubmed ID: 22392984 Computer Controlled Cryo-electron Microscopy--TOM² a Software Package for High-throughput Applications Journal of Structural Biology. Sep, 2011 | Pubmed ID: 21704708 Micromachining Tools and Correlative Approaches for Cellular Cryo-electron Tomography Journal of Structural Biology. Nov, 2010 | Pubmed ID: 20178848 Graphene Oxide: a Substrate for Optimizing Preparations of Frozen-hydrated Samples Journal of Structural Biology. Apr, 2010 | Pubmed ID: 20035878 Insights into the Molecular Architecture of the 26S Proteasome Proceedings of the National Academy of Sciences of the United States of America. Jul, 2009 | Pubmed ID: 19581588 Correlative Cryo-light Microscopy and Cryo-electron Tomography: from Cellular Territories to Molecular Landscapes Current Opinion in Biotechnology. Feb, 2009 | Pubmed ID: 19345086 Automated Cryoelectron Microscopy of "single Particles" Applied to the 26S Proteasome FEBS Letters. Jun, 2007 | Pubmed ID: 17531228 Structural Basis for Subunit Assembly in UDP-glucose Pyrophosphorylase from Saccharomyces Cerevisiae Journal of Molecular Biology. Dec, 2006 | Pubmed ID: 17010990 TOM Software Toolbox: Acquisition and Analysis for Electron Tomography Journal of Structural Biology. Mar, 2005 | Pubmed ID: 15721576 Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography Science (New York, N.Y.). Nov, 2004 | Pubmed ID: 15514115 Preparação de amostras por fresagem de feixe de íons com foco correlativo 3D para tomografia crioeletrônica de alta resolução Anna Bieber*1, Cristina Capitanio*1, Florian Wilfling1,2, Jürgen Plitzko1, Philipp S. Erdmann1,3 1Max Planck Institute of Biochemistry, 2Max Planck Institute for Biophysics, 3Fondazione Human Technopole JoVE 62886 Biology
Preparação de amostras por fresagem de feixe de íons com foco correlativo 3D para tomografia crioeletrônica de alta resolução Anna Bieber*1, Cristina Capitanio*1, Florian Wilfling1,2, Jürgen Plitzko1, Philipp S. Erdmann1,3 1Max Planck Institute of Biochemistry, 2Max Planck Institute for Biophysics, 3Fondazione Human Technopole JoVE 62886 Biology