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Articles by J. Vladimir Sandoval-Sierra in JoVE
Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples M. Angélica Bello1, Yolanda Ruiz-León2, J. Vladimir Sandoval-Sierra3, Svetlana Rezinciuc4, Javier Diéguez-Uribeondo3 1Biodiversity and Conservation Department, Real Jardín Botánico, CSIC, 2Research Support Unit, Real Jardín Botánico, CSIC, 3Mycology Department, Real Jardín Botánico, CSIC, 4Division of Glycoscience, AlbaNova University Center, Royal Institute of Technology (KTH) Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.