Articles by Jaeger Davis in JoVE
Isolamento de células endoteliais da veia umbilical humana (HUVEC) Jaeger Davis1, Steve P. Crampton1, Christopher C.W. Hughes1 1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI) Este protocolo de vídeo ilustra o isolamento e cultura de células endoteliais da veia umbilical humana (HUVEC) de cordão umbilical humano. Uma vez isolados essas células podem ser usadas em ensaios de angiogênese in vitro como o Gel de fibrina Optimized Bead Assay também demonstrada pelo laboratório Hughes.
Otimizado de fibrina Gel Assay Bead para o Estudo da angiogênese Martin N. Nakatsu1, Jaeger Davis1, Christopher C.W. Hughes1 1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI) Este vídeo demonstra o protocolo de um ensaio in vitro angiogênese que recapitula vários estágios de angiogênese. Lapso de tempo imagens de germinação, formação de lúmen, ramificação e anastomose - características-chave da angiogênese - são mostrados.
Other articles by Jaeger Davis on PubMed
TNF Primes Endothelial Cells for Angiogenic Sprouting by Inducing a Tip Cell Phenotype Blood. May, 2008 | Pubmed ID: 18337563 Pathological angiogenesis associated with wound healing often occurs subsequent to an inflammatory response that includes the secretion of cytokines such as tumor necrosis factor (TNF). Controversy exists on the angiogenic actions of TNF, with it being generally proangiogenic in vivo, but antiangiogenic in vitro. We find that whereas continuous administration of TNF in vitro or in vivo inhibits angiogenic sprouting, a 2- to 3-day pulse stimulates angiogenesis by inducing an endothelial "tip cell" phenotype. TNF induces the known tip cell genes platelet-derived growth factor B (PDGFB) and vascular endothelial cell growth factor receptor-2 (VEGFR2), while at the same time blocking signaling through VEGFR2, thus delaying the VEGF-driven angiogenic response. Notch signaling regulates tip cell function, and we find that TNF also induces the notch ligand jagged-1, through an NFkappaB-dependent mechanism. Enrichment of jagged-1 in tip cells was confirmed by immunofluorescent staining as well as by laser capture microdissection/quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) of tip cells sprouting in vitro. Thus, in angiogenesis, the temporal expression of TNF is critical: it delays angiogenesis initially by blocking signaling through VEGFR2, but in addition by inducing a tip cell phenotype through an NFkappaB-dependent pathway, it concomitantly primes endothelial cells (ECs) for sprouting once the initial inflammatory wave has passed.
Cdkn2a is an Atherosclerosis Modifier Locus That Regulates Monocyte/macrophage Proliferation Arteriosclerosis, Thrombosis, and Vascular Biology. Nov, 2011 | Pubmed ID: 21868699 Common genetic variants in a 58-kb region of chromosome 9p21, near the CDKN2A/CDKN2B tumor suppressor locus, are strongly associated with coronary artery disease. However, the underlying mechanism of action remains unknown.