Articles by Jaeger Davis in JoVE
İnsan umbilikal ven endotel hücreleri İzolasyon (HUVEC) Jaeger Davis1, Steve P. Crampton1, Christopher C.W. Hughes1 1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI) Bu video protokolü insan göbek kordonu, insan umbilikal ven endotel hücreleri (HUVEC) izolasyonu ve kültürü göstermektedir. Bir kere bu hücreler, in vitro Hughes laboratuvar gösterdiği Optimize Fibrin Jel Boncuk Testi gibi anjiyogenez tayinlerde için kullanılabilir izole edilmiştir.
Anjiyogenez Çalışmaları için optimize edilmiş Fibrin Jel Boncuk Testi Martin N. Nakatsu1, Jaeger Davis1, Christopher C.W. Hughes1 1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI) Bu video in vitro anjiyogenez tayininde bir protokol anjiyogenez özetlediği çeşitli aşamaları olduğunu göstermektedir. Zaman atlamalı çimlenme görüntüleri, lümen oluşumu, dallanma ve anastomoz - anjiyogenez temel özellikleri gösterilmektedir.
Other articles by Jaeger Davis on PubMed
TNF Primes Endothelial Cells for Angiogenic Sprouting by Inducing a Tip Cell Phenotype Blood. May, 2008 | Pubmed ID: 18337563 Pathological angiogenesis associated with wound healing often occurs subsequent to an inflammatory response that includes the secretion of cytokines such as tumor necrosis factor (TNF). Controversy exists on the angiogenic actions of TNF, with it being generally proangiogenic in vivo, but antiangiogenic in vitro. We find that whereas continuous administration of TNF in vitro or in vivo inhibits angiogenic sprouting, a 2- to 3-day pulse stimulates angiogenesis by inducing an endothelial "tip cell" phenotype. TNF induces the known tip cell genes platelet-derived growth factor B (PDGFB) and vascular endothelial cell growth factor receptor-2 (VEGFR2), while at the same time blocking signaling through VEGFR2, thus delaying the VEGF-driven angiogenic response. Notch signaling regulates tip cell function, and we find that TNF also induces the notch ligand jagged-1, through an NFkappaB-dependent mechanism. Enrichment of jagged-1 in tip cells was confirmed by immunofluorescent staining as well as by laser capture microdissection/quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) of tip cells sprouting in vitro. Thus, in angiogenesis, the temporal expression of TNF is critical: it delays angiogenesis initially by blocking signaling through VEGFR2, but in addition by inducing a tip cell phenotype through an NFkappaB-dependent pathway, it concomitantly primes endothelial cells (ECs) for sprouting once the initial inflammatory wave has passed.
Cdkn2a is an Atherosclerosis Modifier Locus That Regulates Monocyte/macrophage Proliferation Arteriosclerosis, Thrombosis, and Vascular Biology. Nov, 2011 | Pubmed ID: 21868699 Common genetic variants in a 58-kb region of chromosome 9p21, near the CDKN2A/CDKN2B tumor suppressor locus, are strongly associated with coronary artery disease. However, the underlying mechanism of action remains unknown.