Other Publications (1)
Articles by Jean-Michel Vandeweerd in JoVE
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice Jean-Michel Vandeweerd1, Fanny Hontoir1, Alexis De Knoop1, Kathleen De Swert2, Charles Nicaise2 1URVI, NARILIS, Université de Namur, 2URPhyM, NARILIS, Université de Namur Here, we describe a protocol for identifying phrenic motor neurons in mice after intrapleural delivery of fluorophore conjugated cholera toxin subunit beta. Two techniques are compared to inject the pleural cavity: transdiaphragmatic versus transthoracic approaches.
Other articles by Jean-Michel Vandeweerd on PubMed
Prevalence of Age-Related Changes in Ovine Lumbar Intervertebral Discs During Computed Tomography and Magnetic Resonance Imaging Comparative Medicine. | Pubmed ID: 27538861 Ovine models are used to study intervertebral disc (IVD) degeneration. The objective of the current study was to assess the naturally occurring age-related changes of the IVD that can be diagnosed by CT and MRI in the lumbar spine of sheep. We used CT and T2-weighted MR images to score the IVD (L6S1 to L1L2) in 41 sheep (age, 6 mo to 11 y) that were euthanized for reasons not related to musculoskeletal disease. T2 mapping and measurement of T2 time of L6S1 to L2L3 were performed in 22 of the sheep. Degenerative changes manifested as early as 2 y of age and occurred at every IVD level. Discs were more severely damaged in older sheep. The age effect of the L6S1 IVD was larger than the average age effect for the other IVD. The current study provides evidence that lesions similar to those encountered in humans can be identified by CT and MRI in lumbar spine of sheep. Ideally, research animals should be assessed at the initiation of preclinical trials to determine the extent of prevalent degenerative changes. The ovine lumbosacral disc seems particularly prone to degeneration and might be a favorable anatomic site for studying IVD degeneration.