In JoVE (1)
Other Publications (1)
Articles by Johannes Lambrecht in JoVE
Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities Johannes Lambrecht1, Florian Schattenberg1, Hauke Harms1, Susann Mueller1 1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.
Other articles by Johannes Lambrecht on PubMed
Flow Cytometric Quantification, Sorting and Sequencing of Methanogenic Archaea Based on F Autofluorescence Microbial Cell Factories. Oct, 2017 | Pubmed ID: 29084543 The widely established production of CH from renewable biomass in industrial scale anaerobic reactors may play a major role in the future energy supply. It relies on methanogenic archaea as key organisms which represent the bottleneck in the process. The quantitative analysis of these organisms can help to maximize process performance, uncover disturbances before failure, and may ultimately lead to community-based process control schemes. Existing qPCR and fluorescence microscopy-based methods are very attractive but can be cost-intensive and laborious.