John W. Mellors Division of Infectious Diseases University of Pittsburgh Biography Publications Institution JoVE Articles John W. Mellors has not added a biography. If you are John W. Mellors and would like to personalize this page please email our Author Liaison for assistance. Publications New Tools for Quantifying HIV-1 Reservoirs: Plasma RNA Single Copy Assays and Beyond Current HIV/AIDS Reports. Mar, 2012 | Pubmed ID: 22215419 No Effect of Raltegravir Intensification on Viral Replication Markers in the Blood of HIV-1-Infected Patients Receiving Antiretroviral Therapy Journal of Acquired Immune Deficiency Syndromes (1999). Mar, 2012 | Pubmed ID: 22083073 Short-course Raltegravir Intensification Does Not Increase 2 Long Terminal Repeat Episomal HIV-1 DNA in Patients on Effective Antiretroviral Therapy Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America. Feb, 2012 | Pubmed ID: 22016501 Substrate Mimicry: HIV-1 Reverse Transcriptase Recognizes 6-modified-3'-azido-2',3'-dideoxyguanosine-5'-triphosphates As Adenosine Analogs Nucleic Acids Research. Jan, 2012 | Pubmed ID: 21914723 Short-course Combivir After Single-dose Nevirapine Reduces but Does Not Eliminate the Emergence of Nevirapine Resistance in Women Antiviral Therapy. Nov, 2011 | Pubmed ID: 22293443 Initial Viral Decay to Assess the Relative Antiretroviral Potency of Protease Inhibitor-sparing, Nonnucleoside Reverse Transcriptase Inhibitor-sparing, and Nucleoside Reverse Transcriptase Inhibitor-sparing Regimens for First-line Therapy of HIV Infection AIDS (London, England). Nov, 2011 | Pubmed ID: 21941167 Single Genome Sequencing of HIV-1 Gag and Protease Resistance Mutations at Virologic Failure During the OK04 Trial of Simplified Versus Standard Maintenance Therapy Antiviral Therapy. 2011 | Pubmed ID: 21817194 Synthesis, Antiviral Activity, Cytotoxicity and Cellular Pharmacology of L-3'-azido-2',3'-dideoxypurine Nucleosides European Journal of Medicinal Chemistry. Sep, 2011 | Pubmed ID: 21700368 Majority of CD4+ T Cells from Peripheral Blood of HIV-1-infected Individuals Contain Only One HIV DNA Molecule Proceedings of the National Academy of Sciences of the United States of America. Jul, 2011 | Pubmed ID: 21690402 The Base Component of 3'-azido-2',3'-dideoxynucleosides Influences Resistance Mutations Selected in HIV-1 Reverse Transcriptase Antimicrobial Agents and Chemotherapy. Aug, 2011 | Pubmed ID: 21646480 Role of Low-frequency HIV-1 Variants in Failure of Nevirapine-containing Antiviral Therapy in Women Previously Exposed to Single-dose Nevirapine Proceedings of the National Academy of Sciences of the United States of America. May, 2011 | Pubmed ID: 21576473 Inhibitors of Histone Deacetylases: Correlation Between Isoform Specificity and Reactivation of HIV Type 1 (HIV-1) from Latently Infected Cells The Journal of Biological Chemistry. Jun, 2011 | Pubmed ID: 21531716 Factors Influencing the Emergence and Spread of HIV Drug Resistance Arising from Rollout of Antiretroviral Pre-exposure Prophylaxis (PrEP) PloS One. 2011 | Pubmed ID: 21525976 Protease Inhibitor Resistance Is Uncommon in HIV-1 Subtype C Infected Patients on Failing Second-Line Lopinavir/r-Containing Antiretroviral Therapy in South Africa AIDS Research and Treatment. 2011 | Pubmed ID: 21490784 HIV and Hepatitis Virus Resistance to Antivirals: Review of Data from the XIX International HIV and Hepatitis Virus Drug Resistance Workshop and Curative Strategies Antiviral Therapy. 2011 | Pubmed ID: 21447877 Failure of Initial Therapy with Two Nucleosides and Efavirenz is Not Associated with Early Emergence of Mutations in the C-terminus of HIV-1 Reverse Transcriptase Journal of Acquired Immune Deficiency Syndromes (1999). Apr, 2011 | Pubmed ID: 21350368 Genetic Diversity of Simian Immunodeficiency Virus Encoding HIV-1 Reverse Transcriptase Persists in Macaques Despite Antiretroviral Therapy Journal of Virology. Jan, 2011 | Pubmed ID: 21084490 A Randomized, Placebo-controlled Trial of Abacavir Intensification in HIV-1-infected Adults with Virologic Suppression on a Protease Inhibitor-containing Regimen HIV Clinical Trials. Nov-Dec, 2010 | Pubmed ID: 21239359 Antiretroviral Therapies in Women After Single-dose Nevirapine Exposure The New England Journal of Medicine. Oct, 2010 | Pubmed ID: 20942666 HIV-1 Continues to Replicate and Evolve in Patients with Natural Control of HIV Infection Journal of Virology. Dec, 2010 | Pubmed ID: 20926564 The Effect of Raltegravir Intensification on Low-level Residual Viremia in HIV-infected Patients on Antiretroviral Therapy: a Randomized Controlled Trial PLoS Medicine. 2010 | Pubmed ID: 20711481 Planning for Pre-exposure Prophylaxis to Prevent HIV Transmission: Challenges and Opportunities Journal of the International AIDS Society. 2010 | Pubmed ID: 20624303 Clonal Resistance Analyses of HIV Type-1 After Failure of Therapy with Didanosine, Lamivudine and Tenofovir Antiviral Therapy. 2010 | Pubmed ID: 20516563 A Pilot Study to Determine the Impact on Dyslipidemia of Adding Tenofovir to Stable Background Antiretroviral Therapy: ACTG 5206 AIDS (London, England). Jul, 2010 | Pubmed ID: 20495438 Low Frequency Nonnucleoside Reverse-transcriptase Inhibitor-resistant Variants Contribute to Failure of Efavirenz-containing Regimens in Treatment- Experienced Patients The Journal of Infectious Diseases. Mar, 2010 | Pubmed ID: 20102272 Synthesis and Evaluation of 3'-azido-2',3'-dideoxypurine Nucleosides As Inhibitors of Human Immunodeficiency Virus Bioorganic & Medicinal Chemistry Letters. Jan, 2010 | Pubmed ID: 19948402 Optimization of Allele-specific PCR Using Patient-specific HIV Consensus Sequences for Primer Design Journal of Virological Methods. Mar, 2010 | Pubmed ID: 19948190 Varied Patterns of HIV-1 Drug Resistance on Failing First-line Antiretroviral Therapy in South Africa Journal of Acquired Immune Deficiency Syndromes (1999). Apr, 2010 | Pubmed ID: 19801944 Progress in Basic and Clinical Research on HIV Resistance: Report on the XVIII International HIV Drug Resistance Workshop Antiviral Therapy. 2009 | Pubmed ID: 19918107 RT-SHIV Subpopulation Dynamics in Infected Macaques During Anti-HIV Therapy Retrovirology. 2009 | Pubmed ID: 19889213 Anti-human Immunodeficiency Virus Activity, Cross-resistance, Cytotoxicity, and Intracellular Pharmacology of the 3'-azido-2',3'-dideoxypurine Nucleosides Antimicrobial Agents and Chemotherapy. Sep, 2009 | Pubmed ID: 19596885 Metabolic Outcomes in a Randomized Trial of Nucleoside, Nonnucleoside and Protease Inhibitor-sparing Regimens for Initial HIV Treatment AIDS (London, England). Jun, 2009 | Pubmed ID: 19417580 Simplification Strategies to Reduce Antiretroviral Drug Exposure: Progress and Prospects Antiviral Therapy. 2009 | Pubmed ID: 19320232 Regimen Simplification to Atazanavir-ritonavir Alone As Maintenance Antiretroviral Therapy: Final 48-week Clinical and Virologic Outcomes The Journal of Infectious Diseases. Mar, 2009 | Pubmed ID: 19191590 Single-dose Nevirapine to Prevent Mother-to-child Transmission of HIV Type 1: Balancing the Benefits and Risks Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America. Feb, 2009 | Pubmed ID: 19133800 Antiretroviral Drug Resistance Testing in Adult HIV-1 Infection: 2008 Recommendations of an International AIDS Society-USA Panel Topics in HIV Medicine : a Publication of the International AIDS Society, USA. Aug-Sep, 2008 | Pubmed ID: 19856548 Broad Advances in Understanding HIV Resistance to Antiretrovirals: Report on the XVII International HIV Drug Resistance Workshop Antiviral Therapy. 2008 | Pubmed ID: 19195337 Mechanism by Which a Glutamine to Leucine Substitution at Residue 509 in the Ribonuclease H Domain of HIV-1 Reverse Transcriptase Confers Zidovudine Resistance Biochemistry. Dec, 2008 | Pubmed ID: 19067547 Lytic Granule Loading of CD8+ T Cells is Required for HIV-infected Cell Elimination Associated with Immune Control Immunity. Dec, 2008 | Pubmed ID: 19062316 YKL-40, a Marker of Simian Immunodeficiency Virus Encephalitis, Modulates the Biological Activity of Basic Fibroblast Growth Factor The American Journal of Pathology. Jul, 2008 | Pubmed ID: 18556781 Antiretroviral Drug Resistance Testing in Adult HIV-1 Infection: 2008 Recommendations of an International AIDS Society-USA Panel Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America. Jul, 2008 | Pubmed ID: 18549313 Class-sparing Regimens for Initial Treatment of HIV-1 Infection The New England Journal of Medicine. May, 2008 | Pubmed ID: 18480202 Randomized Study of Dual Versus Single Ritonavir-enhanced Protease Inhibitors for Protease Inhibitor-experienced Patients with HIV HIV Clinical Trials. Mar-Apr, 2008 | Pubmed ID: 18474494 Low-level Viremia Persists for at Least 7 Years in Patients on Suppressive Antiretroviral Therapy Proceedings of the National Academy of Sciences of the United States of America. Mar, 2008 | Pubmed ID: 18332425 Frequent Polymorphism at Drug Resistance Sites in HIV-1 Protease and Reverse Transcriptase AIDS (London, England). Feb, 2008 | Pubmed ID: 18301062 Madurahydroxylactone Derivatives As Dual Inhibitors of Human Immunodeficiency Virus Type 1 Integrase and RNase H Antimicrobial Agents and Chemotherapy. Jan, 2008 | Pubmed ID: 17967911 Mechanisms by Which the G333D Mutation in Human Immunodeficiency Virus Type 1 Reverse Transcriptase Facilitates Dual Resistance to Zidovudine and Lamivudine Antimicrobial Agents and Chemotherapy. Jan, 2008 | Pubmed ID: 17967907 The Use of Beta-D-2,6-diaminopurine Dioxolane with or Without Mycophenolate Mofetil in Drug-resistant HIV Infection AIDS (London, England). Oct, 2007 | Pubmed ID: 17885292 Potential Impact of Antiretroviral Chemoprophylaxis on HIV-1 Transmission in Resource-limited Settings PloS One. 2007 | Pubmed ID: 17878928 Suppression of Viremia and Evolution of Human Immunodeficiency Virus Type 1 Drug Resistance in a Macaque Model for Antiretroviral Therapy Journal of Virology. Nov, 2007 | Pubmed ID: 17855539 Molecular Mechanisms of Bidirectional Antagonism Between K65R and Thymidine Analog Mutations in HIV-1 Reverse Transcriptase AIDS (London, England). Jul, 2007 | Pubmed ID: 17589186 Prognostic Value of HIV-1 RNA, CD4 Cell Count, and CD4 Cell Count Slope for Progression to AIDS and Death in Untreated HIV-1 Infection JAMA : the Journal of the American Medical Association. Jun, 2007 | Pubmed ID: 17551128 Selection of Mutations in the Connection and RNase H Domains of Human Immunodeficiency Virus Type 1 Reverse Transcriptase That Increase Resistance to 3'-azido-3'-dideoxythymidine Journal of Virology. Aug, 2007 | Pubmed ID: 17507476 ART Suppresses Plasma HIV-1 RNA to a Stable Set Point Predicted by Pretherapy Viremia PLoS Pathogens. Apr, 2007 | Pubmed ID: 17411338 No Cure Yet for HIV-1, but Therapeutic Research Presses On The Journal of Infectious Diseases. Mar, 2007 | Pubmed ID: 17299705 Molecular Mechanism by Which the K70E Mutation in Human Immunodeficiency Virus Type 1 Reverse Transcriptase Confers Resistance to Nucleoside Reverse Transcriptase Inhibitors Antimicrobial Agents and Chemotherapy. Jan, 2007 | Pubmed ID: 17088490 The Level of Persistent HIV Viremia Does Not Increase After Successful Simplification of Maintenance Therapy to Lopinavir/ritonavir Alone AIDS (London, England). Nov, 2006 | Pubmed ID: 17117019 A Randomized Trial of Treatment Interruption Before Optimized Antiretroviral Therapy for Persons with Drug-resistant HIV: 48-week Virologic Results of ACTG A5086 The Journal of Infectious Diseases. Nov, 2006 | Pubmed ID: 17041858 Regimen Simplification to Atazanavir-ritonavir Alone As Maintenance Antiretroviral Therapy After Sustained Virologic Suppression JAMA : the Journal of the American Medical Association. Aug, 2006 | Pubmed ID: 16905786 Antagonism Between the HIV-1 Reverse-transcriptase Mutation K65R and Thymidine-analogue Mutations at the Genomic Level The Journal of Infectious Diseases. Sep, 2006 | Pubmed ID: 16897664 Blinded, Multicenter Comparison of Methods to Detect a Drug-resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency Journal of Clinical Microbiology. Jul, 2006 | Pubmed ID: 16825395 Potential Impact of Antiretroviral Therapy on HIV-1 Transmission and AIDS Mortality in Resource-limited Settings Journal of Acquired Immune Deficiency Syndromes (1999). Apr, 2006 | Pubmed ID: 16652038 The K65R Mutation in Human Immunodeficiency Virus Type 1 Reverse Transcriptase Exhibits Bidirectional Phenotypic Antagonism with Thymidine Analog Mutations Journal of Virology. May, 2006 | Pubmed ID: 16641288 Reverse Transcriptase Mutations 118I, 208Y, and 215Y Cause HIV-1 Hypersusceptibility to Non-nucleoside Reverse Transcriptase Inhibitors AIDS (London, England). Apr, 2006 | Pubmed ID: 16603849 Selection and Persistence of Non-nucleoside Reverse Transcriptase Inhibitor-resistant HIV-1 in Patients Starting and Stopping Non-nucleoside Therapy AIDS (London, England). Mar, 2006 | Pubmed ID: 16514300 Sensitive Phenotypic Detection of Minor Drug-resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Variants Journal of Clinical Microbiology. Nov, 2005 | Pubmed ID: 16272507 In Vitro Selection and Analysis of Human Immunodeficiency Virus Type 1 Resistant to Derivatives of Beta-2',3'-didehydro-2',3'-dideoxy-5-fluorocytidine Antimicrobial Agents and Chemotherapy. Sep, 2005 | Pubmed ID: 16127074 Depletion of Latent HIV-1 Infection in Vivo: a Proof-of-concept Study Lancet. Aug 13-19, 2005 | Pubmed ID: 16099290 The 3'-azido Group is Not the Primary Determinant of 3'-azido-3'-deoxythymidine (AZT) Responsible for the Excision Phenotype of AZT-resistant HIV-1 The Journal of Biological Chemistry. Aug, 2005 | Pubmed ID: 15970587 In Vitro Activity of Structurally Diverse Nucleoside Analogs Against Human Immunodeficiency Virus Type 1 with the K65R Mutation in Reverse Transcriptase Antimicrobial Agents and Chemotherapy. Mar, 2005 | Pubmed ID: 15728915 Mechanism for Nucleoside Analog-mediated Abrogation of HIV-1 Replication: Balance Between RNase H Activity and Nucleotide Excision Proceedings of the National Academy of Sciences of the United States of America. Feb, 2005 | Pubmed ID: 15684061 Combining Fosamprenavir with Lopinavir/ritonavir Substantially Reduces Amprenavir and Lopinavir Exposure: ACTG Protocol A5143 Results AIDS (London, England). Jan, 2005 | Pubmed ID: 15668539 Multiple, Linked Human Immunodeficiency Virus Type 1 Drug Resistance Mutations in Treatment-experienced Patients Are Missed by Standard Genotype Analysis Journal of Clinical Microbiology. Jan, 2005 | Pubmed ID: 15635002 Prognostic Value of Plasma HIV RNA Among Highly Active Antiretroviral Therapy Users AIDS (London, England). Dec, 2004 | Pubmed ID: 15622318 Genetic Correlates of Efavirenz Hypersusceptibility AIDS (London, England). Sep, 2004 | Pubmed ID: 15316338 Hybrids of [TSAO-T]-[foscarnet]: The First Conjugate of Foscarnet with a Non-nucleoside Reverse Transcriptase Inhibitor Through a Labile Covalent Ester Bond Journal of Medicinal Chemistry. Jun, 2004 | Pubmed ID: 15189038 A Randomized Trial of Nelfinavir and Abacavir in Combination with Efavirenz and Adefovir Dipivoxil in HIV-1-infected Persons with Virological Failure Receiving Indinavir Antiviral Therapy. Dec, 2003 | Pubmed ID: 14760884 Six-year Follow-up of HIV-1-infected Adults in a Clinical Trial of Antiretroviral Therapy with Indinavir, Zidovudine, and Lamivudine AIDS (London, England). Nov, 2003 | Pubmed ID: 14571186 New Real-time Reverse Transcriptase-initiated PCR Assay with Single-copy Sensitivity for Human Immunodeficiency Virus Type 1 RNA in Plasma Journal of Clinical Microbiology. Oct, 2003 | Pubmed ID: 14532178 Questions to and Answers from the International AIDS Society-USA Resistance Testing Guidelines Panel Topics in HIV Medicine : a Publication of the International AIDS Society, USA. Jul-Aug, 2003 | Pubmed ID: 12876334 Antiretroviral Drug Resistance Testing in Adults Infected with Human Immunodeficiency Virus Type 1: 2003 Recommendations of an International AIDS Society-USA Panel Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America. Jul, 2003 | Pubmed ID: 12830416 Relationship Between 3'-azido-3'-deoxythymidine Resistance and Primer Unblocking Activity in Foscarnet-resistant Mutants of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Journal of Virology. Jun, 2003 | Pubmed ID: 12743270 Accuracy of the TRUGENE HIV-1 Genotyping Kit Journal of Clinical Microbiology. Apr, 2003 | Pubmed ID: 12682149 Virologic and Immunologic Values Allowing Safe Deferral of Antiretroviral Therapy AIDS (London, England). Dec, 2002 | Pubmed ID: 12461420 Interruption of Antiretroviral Therapy to Augment Immune Control of Chronic HIV-1 Infection: Risk Without Reward Proceedings of the National Academy of Sciences of the United States of America. Oct, 2002 | Pubmed ID: 12370421 Antiretroviral-drug Resistance Among Patients Recently Infected with HIV The New England Journal of Medicine. Aug, 2002 | Pubmed ID: 12167680 Dual Vs Single Protease Inhibitor Therapy Following Antiretroviral Treatment Failure: a Randomized Trial JAMA : the Journal of the American Medical Association. Jul, 2002 | Pubmed ID: 12095381 Effects of Specific Zidovudine Resistance Mutations and Substrate Structure on Nucleotide-dependent Primer Unblocking by Human Immunodeficiency Virus Type 1 Reverse Transcriptase Antimicrobial Agents and Chemotherapy. May, 2002 | Pubmed ID: 11959594 Recovery of Replication-competent HIV Type 1-infected Circulating Monocytes from Individuals Receiving Antiretroviral Therapy AIDS Research and Human Retroviruses. Apr, 2002 | Pubmed ID: 11958686 Predictive Value of Immunologic and Virologic Markers After Long or Short Duration of HIV-1 Infection Journal of Acquired Immune Deficiency Syndromes (1999). Apr, 2002 | Pubmed ID: 11917238 Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays Helene Mens1, Mary Kearney1, Ann Wiegand1, Jonathan Spindler1, Frank Maldarelli1, John W. Mellors2, John M. Coffin3 1The virology Core at the HIV Drug Resistance Program, NCI-Frederick, 2Division of Infectious Diseases, University of Pittsburgh, 3Department of Molecular Biology and Microbiology, Tuffts University JoVE 2960 Immunology and Infection
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays Helene Mens1, Mary Kearney1, Ann Wiegand1, Jonathan Spindler1, Frank Maldarelli1, John W. Mellors2, John M. Coffin3 1The virology Core at the HIV Drug Resistance Program, NCI-Frederick, 2Division of Infectious Diseases, University of Pittsburgh, 3Department of Molecular Biology and Microbiology, Tuffts University JoVE 2960 Immunology and Infection