Jonathon Howard

Publications

• In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Reflection Microscopy
  Methods in Molecular Biology (Clifton, N.J.). 2022  |   Pubmed ID: 35476326
• Counting Fluorescently Labeled Proteins in Tissues in the Spinning Disk Microscope Using Single-molecule Calibrations
  Molecular Biology of the Cell. Mar, 2022  |   Pubmed ID: 35323029
• Structures of Outer-arm Dynein Array on Microtubule Doublet Reveal a Motor Coordination Mechanism
  Nature Structural & Molecular Biology. 10, 2021  |   Pubmed ID: 34556869
• The Narrowing of Dendrite Branches Across Nodes Follows a Well-defined Scaling Law
  Proceedings of the National Academy of Sciences of the United States of America. 07, 2021  |   Pubmed ID: 34215693
• Focal Laser Stimulation of Fly Nociceptors Activates Distinct Axonal and Dendritic Ca Signals
  Biophysical Journal. 08, 2021  |   Pubmed ID: 34175294
• Physical Bioenergetics: Energy Fluxes, Budgets, and Constraints in Cells
  Proceedings of the National Academy of Sciences of the United States of America. 06, 2021  |   Pubmed ID: 34140336
• Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes
  Trends in Cell Biology. 01, 2021  |   Pubmed ID: 33183955
• Purification of Ciliary Tubulin from Chlamydomonas Reinhardtii
  Current Protocols in Protein Science. 06, 2020  |   Pubmed ID: 32568459
• Contribution of Increasing Plasma Membrane to the Energetic Cost of Early Zebrafish Embryogenesis
  Molecular Biology of the Cell. 03, 2020  |   Pubmed ID: 32049586
• Three Beads Are Better Than One
  Biophysical Journal. 01, 2020  |   Pubmed ID: 31951531
• Predicted Effects of Severing Enzymes on the Length Distribution and Total Mass of Microtubules
  Biophysical Journal. 12, 2019  |   Pubmed ID: 31708162
• The Kinetics of Nucleotide Binding to Isolated Chlamydomonas Axonemes Using UV-TIRF Microscopy
  Biophysical Journal. 08, 2019  |   Pubmed ID: 31400919
• Nicotinamide Adenine Dinucleotides and Their Precursor NMN Have No Direct Effect on Microtubule Dynamics in Purified Brain Tubulin
  PloS One. 2019  |   Pubmed ID: 31393939
• Force Generated by Two Kinesin Motors Depends on the Load Direction and Intermolecular Coupling
  Physical Review Letters. May, 2019  |   Pubmed ID: 31144901
• The Dynamic and Structural Properties of Axonemal Tubulins Support the High Length Stability of Cilia
  Nature Communications. 04, 2019  |   Pubmed ID: 31015426
• Spastin is a Dual-function Enzyme That Severs Microtubules and Promotes Their Regrowth to Increase the Number and Mass of Microtubules
  Proceedings of the National Academy of Sciences of the United States of America. 03, 2019  |   Pubmed ID: 30837315
• Heat Oscillations Driven by the Embryonic Cell Cycle Reveal the Energetic Costs of Signaling
  Developmental Cell. Mar, 2019  |   Pubmed ID: 30713074
• Label-free High-speed Wide-field Imaging of Single Microtubules Using Interference Reflection Microscopy
  Journal of Microscopy. Oct, 2018  |   Pubmed ID: 30044498
• Structural Biology: Piezo Senses Tension Through Curvature
  Current Biology : CB. Apr, 2018  |   Pubmed ID: 29689211
• Physical Limits on the Precision of Mitotic Spindle Positioning by Microtubule Pushing Forces: Mechanics of Mitotic Spindle Positioning
  BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. 11, 2017  |   Pubmed ID: 28960439
• Structural Biology: A Force-Sensitive Ion Channel Springs to Life
  Current Biology : CB. 09, 2017  |   Pubmed ID: 28950085
• Curvature Regulation of the Ciliary Beat Through Axonemal Twist
  Physical Review. E. Oct, 2016  |   Pubmed ID: 27841522
• Mechanism of Microtubule Lumen Entry for the α-tubulin Acetyltransferase Enzyme αTAT1
  Proceedings of the National Academy of Sciences of the United States of America. 11, 2016  |   Pubmed ID: 27803321
• The Mitotic Spindle in the One-Cell C. Elegans Embryo Is Positioned with High Precision and Stability
  Biophysical Journal. Oct, 2016  |   Pubmed ID: 27760363
• A Force-generating Machinery Maintains the Spindle at the Cell Center During Mitosis
  Science (New York, N.Y.). May, 2016  |   Pubmed ID: 27230381
• Dynamic Curvature Regulation Accounts for the Symmetric and Asymmetric Beats of Chlamydomonas Flagella
  ELife. 05, 2016  |   Pubmed ID: 27166516
• Broken Detailed Balance at Mesoscopic Scales in Active Biological Systems
  Science (New York, N.Y.). Apr, 2016  |   Pubmed ID: 27126047
• Automatic Optimal Filament Segmentation with Sub-pixel Accuracy Using Generalized Linear Models and B-spline Level-sets
  Medical Image Analysis. 08, 2016  |   Pubmed ID: 27104582
• Microtubules: 50 Years on from the Discovery of Tubulin
  Nature Reviews. Molecular Cell Biology. 04, 2016  |   Pubmed ID: 27103327
• Independent Control of the Static and Dynamic Components of the Chlamydomonas Flagellar Beat
  Current Biology : CB. 04, 2016  |   Pubmed ID: 27040779
• Splicing of Nascent RNA Coincides with Intron Exit from RNA Polymerase II
  Cell. Apr, 2016  |   Pubmed ID: 27020755
• Regulation of Microtubule Growth and Catastrophe: Unifying Theory and Experiment
  Trends in Cell Biology. Dec, 2015  |   Pubmed ID: 26616192
• Kinesin Kip2 Enhances Microtubule Growth in Vitro Through Length-dependent Feedback on Polymerization and Catastrophe
  ELife. Nov, 2015  |   Pubmed ID: 26576948
• The Motility of Axonemal Dynein is Regulated by the Tubulin Code
  Biophysical Journal. Dec, 2014  |   Pubmed ID: 25658008
• The Microtubule-based Cytoskeleton is a Component of a Mechanical Signaling Pathway in Fly Campaniform Receptors
  Biophysical Journal. Dec, 2014  |   Pubmed ID: 25517144
• Quantitative Cell Biology: the Essential Role of Theory
  Molecular Biology of the Cell. Nov, 2014  |   Pubmed ID: 25368416
• Stu2, the Budding Yeast XMAP215/Dis1 Homolog, Promotes Assembly of Yeast Microtubules by Increasing Growth Rate and Decreasing Catastrophe Frequency
  The Journal of Biological Chemistry. Oct, 2014  |   Pubmed ID: 25172511
• Motor Regulation Results in Distal Forces That Bend Partially Disintegrated Chlamydomonas Axonemes into Circular Arcs
  Biophysical Journal. Jun, 2014  |   Pubmed ID: 24896122
• A Brief Scientific Biography of Prof. Alan J. Hunt
  Cellular and Molecular Bioengineering. Dec, 2013  |   Pubmed ID: 24683426