Articles by Katrin Süring in JoVE
Citometria de fluxo de partículas ligadas aos Bet v 1 alérgeno no PM10 Katrin Süring1, Sabine Bach1, Conny Höflich1, Wolfgang Straff1 1Environmental Medicine and Health Effects Assessment, Federal Environment Agency Aqui, apresentamos um protocolo para quantificar partículas carregadas de alérgenos por citometria de fluxo. partículas de material particulado ambiental podem agir como portadores de alérgenos adsorvidos. Mostramos aqui que a citometria de fluxo, um método largamente usado para caracterizar os sólidos em suspensão> 0,5 um de diâmetro, pode ser usado para medir estas partículas carregadas com alérgeno.
Other articles by Katrin Süring on PubMed
Mutations in Bone Morphogenetic Protein Receptor 1B Cause Brachydactyly Type A2 Proceedings of the National Academy of Sciences of the United States of America. Oct, 2003 | Pubmed ID: 14523231 Brachydactyly (BD) type A2 is an autosomal dominant hand malformation characterized by shortening and lateral deviation of the index fingers and, to a variable degree, shortening and deviation of the first and second toes. We performed linkage analysis in two unrelated German families and mapped a locus for BD type A2 to 4q21-q25. This interval includes the gene bone morphogenetic protein receptor 1B (BMPR1B), a type I transmembrane serinethreonine kinase. In one family, we identified a T599 --> A mutation changing an isoleucine into a lysine residue (I200K) within the glycine/serine (GS) domain of BMPR1B, a region involved in phosphorylation of the receptor. In the other family we identified a C1456 --> T mutation leading to an arginine-to-tryptophan amino acid change (R486W) in a highly conserved region C-terminal of the BMPR1B kinase domain. An in vitro kinase assay showed that the I200K mutation is kinase-deficient, whereas the R486W mutation has normal kinase activity, indicating a different pathogenic mechanism. Functional analyses with a micromass culture system revealed a strong inhibition of chondrogenesis by both mutant receptors. Overexpression of mutant chBmpR1b in vivo in chick embryos by using a retroviral system resulted either in a BD phenotype with shortening and/or missing phalanges similar to the human phenotype or in severe hypoplasia of the entire limb. These findings imply that both mutations identified in human BMPR1B affect cartilage formation in a dominant-negative manner.
Holi Colours Contain PM10 and Can Induce Pro-inflammatory Responses Journal of Occupational Medicine and Toxicology (London, England). 2016 | Pubmed ID: 27617025 At Holi festivals, originally celebrated in India but more recently all over the world, people throw coloured powder (Holi powder, Holi colour, Gulal powder) at each other. Adverse health effects, i.e. skin and ocular irritations as well as respiratory problems may be the consequences. The aim of this study was to uncover some of the underlying mechanisms.