Kevin Eggan Stanley Center for Psychiatric Research Broad Institute of MIT and Harvard Biography Publications Institution JoVE Articles Kevin Eggan has not added a biography. If you are Kevin Eggan and would like to personalize this page please email our Author Liaison for assistance. Publications A Multiplexed GRNA PiggyBac Transposon System Facilitates Efficient Induction of CRISPRi and CRISPRa in Human Pluripotent Stem Cells Scientific Reports. Jan, 2020 | Pubmed ID: 31959800 Publisher Correction: Exome Sequencing in Amyotrophic Lateral Sclerosis Implicates a Novel Gene, DNAJC7, Encoding a Heat-shock Protein Nature Neuroscience. Feb, 2020 | Pubmed ID: 31857710 Herpesviral Lytic Gene Functions Render the Viral Genome Susceptible to Novel Editing by CRISPR/Cas9 ELife. 12, 2019 | Pubmed ID: 31789594 Exome Sequencing in Amyotrophic Lateral Sclerosis Implicates a Novel Gene, DNAJC7, Encoding a Heat-shock Protein Nature Neuroscience. 12, 2019 | Pubmed ID: 31768050 RNA-seq As a Tool for Evaluating Human Embryo Competence Genome Research. 10, 2019 | Pubmed ID: 31548358 Cancer-Related Mutations Identified in Primed and Naive Human Pluripotent Stem Cells Cell Stem Cell. Oct, 2019 | Pubmed ID: 31543368 A High-Content Screen Identifies TPP1 and Aurora B As Regulators of Axonal Mitochondrial Transport Cell Reports. Sep, 2019 | Pubmed ID: 31533043 Comparison of Three Congruent Patient-specific Cell Types for the Modelling of a Human Genetic Schwann-cell Disorder Nature Biomedical Engineering. 07, 2019 | Pubmed ID: 30962586 Dysregulated Protocadherin-pathway Activity As an Intrinsic Defect in Induced Pluripotent Stem Cell-derived Cortical Interneurons from Subjects with Schizophrenia Nature Neuroscience. 02, 2019 | Pubmed ID: 30664768 ALS-implicated Protein TDP-43 Sustains Levels of STMN2, a Mediator of Motor Neuron Growth and Repair Nature Neuroscience. 02, 2019 | Pubmed ID: 30643292 A Stem Cell-Based Screening Platform Identifies Compounds That Desensitize Motor Neurons to Endoplasmic Reticulum Stress Molecular Therapy : the Journal of the American Society of Gene Therapy. 01, 2019 | Pubmed ID: 30446391 Convergence of Independent DISC1 Mutations on Impaired Neurite Growth Via Decreased UNC5D Expression Translational Psychiatry. 11, 2018 | Pubmed ID: 30410030 Oligodendrocyte Differentiation of Induced Pluripotent Stem Cells Derived from Subjects with Schizophrenias Implicate Abnormalities in Development Translational Psychiatry. 10, 2018 | Pubmed ID: 30352993 Comparative Genomic Analysis of Embryonic, Lineage-converted and Stem Cell-derived Motor Neurons Development (Cambridge, England). 11, 2018 | Pubmed ID: 30337375 All-optical Synaptic Electrophysiology Probes Mechanism of Ketamine-induced Disinhibition Nature Methods. 10, 2018 | Pubmed ID: 30275587 Hippo Signaling in the Ovary and Polycystic Ovarian Syndrome Journal of Assisted Reproduction and Genetics. Oct, 2018 | Pubmed ID: 30120633 Dipeptide Repeat Proteins Activate a Heat Shock Response Found in C9ORF72-ALS/FTLD Patients Acta Neuropathologica Communications. 07, 2018 | Pubmed ID: 29973287 The C9orf72-interacting Protein Smcr8 is a Negative Regulator of Autoimmunity and Lysosomal Exocytosis Genes & Development. 07, 2018 | Pubmed ID: 29950492 TDP-43 Induces P53-mediated Cell Death of Cortical Progenitors and Immature Neurons Scientific Reports. 05, 2018 | Pubmed ID: 29802307 Combining NGN2 Programming with Developmental Patterning Generates Human Excitatory Neurons with NMDAR-Mediated Synaptic Transmission Cell Reports. 05, 2018 | Pubmed ID: 29791859 All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human IPSC-Derived Motor Neuron Model of ALS Stem Cell Reports. 06, 2018 | Pubmed ID: 29779896 Publisher Correction: Whole Genome Sequencing in Psychiatric Disorders: the WGSPD Consortium Nature Neuroscience. Jul, 2018 | Pubmed ID: 29549319 Whole Genome Sequencing in Psychiatric Disorders: the WGSPD Consortium Nature Neuroscience. 12, 2017 | Pubmed ID: 29184211 Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons Stem Cell Reports. 11, 2017 | Pubmed ID: 29107592 A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease Stem Cell Reports. 10, 2017 | Pubmed ID: 29020615 Reactive Astrocytes Promote ALS-like Degeneration and Intracellular Protein Aggregation in Human Motor Neurons by Disrupting Autophagy Through TGF-β1 Stem Cell Reports. Aug, 2017 | Pubmed ID: 28712846 Assessing the Safety of Human Pluripotent Stem Cells and Their Derivatives for Clinical Applications Stem Cell Reports. Jul, 2017 | Pubmed ID: 28700896 Human Pluripotent Stem Cells Recurrently Acquire and Expand Dominant Negative P53 Mutations Nature. 05, 2017 | Pubmed ID: 28445466 Genetic Ablation of AXL Does Not Protect Human Neural Progenitor Cells and Cerebral Organoids from Zika Virus Infection Cell Stem Cell. Dec, 2016 | Pubmed ID: 27912091 Generation of a TLE3 Heterozygous Knockout Human Embryonic Stem Cell Line Using CRISPR-Cas9 Stem Cell Research. Sep, 2016 | Pubmed ID: 27879221 Generation of a TLE1 Homozygous Knockout Human Embryonic Stem Cell Line Using CRISPR-Cas9 Stem Cell Research. Sep, 2016 | Pubmed ID: 27879218 Two Familial ALS Proteins Function in Prevention/repair of Transcription-associated DNA Damage Proceedings of the National Academy of Sciences of the United States of America. Nov, 2016 | Pubmed ID: 27849576 Monitoring Peripheral Nerve Degeneration in ALS by Label-free Stimulated Raman Scattering Imaging Nature Communications. Oct, 2016 | Pubmed ID: 27796305 Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells Current Protocols in Stem Cell Biology. Aug, 2016 | Pubmed ID: 27532820 Loss-of-function Mutations in the C9ORF72 Mouse Ortholog Cause Fatal Autoimmune Disease Science Translational Medicine. Jul, 2016 | Pubmed ID: 27412785 CAT7 and Cat7l Long Non-coding RNAs Tune Polycomb Repressive Complex 1 Function During Human and Zebrafish Development The Journal of Biological Chemistry. Sep, 2016 | Pubmed ID: 27405765 Modeling ALS with Motor Neurons Derived from Human Induced Pluripotent Stem Cells Nature Neuroscience. Apr, 2016 | Pubmed ID: 27021939 SLC52A3, A Brown-Vialetto-van Laere Syndrome Candidate Gene is Essential for Mouse Development, but Dispensable for Motor Neuron Differentiation Human Molecular Genetics. May, 2016 | Pubmed ID: 26976849 A Perspective on Stem Cell Modeling of Amyotrophic Lateral Sclerosis Cell Cycle (Georgetown, Tex.). 2015 | Pubmed ID: 26505672 Automated, High-throughput Derivation, Characterization and Differentiation of Induced Pluripotent Stem Cells Nature Methods. Sep, 2015 | Pubmed ID: 26237226 From Dish to Bedside: Lessons Learned While Translating Findings from a Stem Cell Model of Disease to a Clinical Trial Cell Stem Cell. Jul, 2015 | Pubmed ID: 26140603 Efficient CRISPR-Cas9-mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus Cell Reports. May, 2015 | Pubmed ID: 25937281 Focus on Induced Pluripotency and Cellular Reprogramming The EMBO Journal. Jun, 2015 | Pubmed ID: 25916828 Generation of Neuropeptidergic Hypothalamic Neurons from Human Pluripotent Stem Cells Development (Cambridge, England). Feb, 2015 | Pubmed ID: 25670790 ALS-causative Mutations in FUS/TLS Confer Gain and Loss of Function by Altered Association with SMN and U1-snRNP Nature Communications. 2015 | Pubmed ID: 25625564 Motoneurons Derived from Induced Pluripotent Stem Cells Develop Mature Phenotypes Typical of Endogenous Spinal Motoneurons The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2015 | Pubmed ID: 25609642 Modeling Pain in Vitro Using Nociceptor Neurons Reprogrammed from Fibroblasts Nature Neuroscience. Jan, 2015 | Pubmed ID: 25420066 IPSC-derived Dopamine Neurons Reveal Differences Between Monozygotic Twins Discordant for Parkinson's Disease Cell Reports. Nov, 2014 | Pubmed ID: 25456120 Genetic Variation in Human DNA Replication Timing Cell. Nov, 2014 | Pubmed ID: 25416942 The Role of Maternal-specific H3K9me3 Modification in Establishing Imprinted X-chromosome Inactivation and Embryogenesis in Mice Nature Communications. 2014 | Pubmed ID: 25394724 Genetic Validation of a Therapeutic Target in a Mouse Model of ALS Science Translational Medicine. Aug, 2014 | Pubmed ID: 25100738 DNA Methylation Dynamics of the Human Preimplantation Embryo Nature. Jul, 2014 | Pubmed ID: 25079558 FUS is Sequestered in Nuclear Aggregates in ALS Patient Fibroblasts Molecular Biology of the Cell. Sep, 2014 | Pubmed ID: 25009283 Notch Inhibition Allows Oncogene-independent Generation of IPS Cells Nature Chemical Biology. Aug, 2014 | Pubmed ID: 24952596 Ketamine Exposure in Early Development Impairs Specification of the Primary Germ Cell Layers Neurotoxicology and Teratology. May-Jun, 2014 | Pubmed ID: 24746641 Pathways Disrupted in Human ALS Motor Neurons Identified Through Genetic Correction of Mutant SOD1 Cell Stem Cell. Jun, 2014 | Pubmed ID: 24704492 Intrinsic Membrane Hyperexcitability of Amyotrophic Lateral Sclerosis Patient-derived Motor Neurons Cell Reports. Apr, 2014 | Pubmed ID: 24703839 Gene Profiling of Human Induced Pluripotent Stem Cell-derived Astrocyte Progenitors Following Spinal Cord Engraftment Stem Cells Translational Medicine. May, 2014 | Pubmed ID: 24604284 Nanog-independent Reprogramming to IPSCs with Canonical Factors Stem Cell Reports. Feb, 2014 | Pubmed ID: 24527385 Axonal Transport of TDP-43 MRNA Granules is Impaired by ALS-causing Mutations Neuron. Feb, 2014 | Pubmed ID: 24507191 How to Make Spinal Motor Neurons Development (Cambridge, England). Feb, 2014 | Pubmed ID: 24449832 Picking the Lock on Pluripotency The New England Journal of Medicine. Nov, 2013 | Pubmed ID: 24283230 The Mouse C9ORF72 Ortholog is Enriched in Neurons Known to Degenerate in ALS and FTD Nature Neuroscience. Dec, 2013 | Pubmed ID: 24185425 Derivation of Induced Pluripotent Stem Cells from the Baboon: a Nonhuman Primate Model for Preclinical Testing of Stem Cell Therapies Cellular Reprogramming. Dec, 2013 | Pubmed ID: 24182315 Opportunities and Challenges of Pluripotent Stem Cell Neurodegenerative Disease Models Nature Neuroscience. Jul, 2013 | Pubmed ID: 23799470 Modeling Human Disease with Pluripotent Stem Cells: from Genome Association to Function Cell Stem Cell. Jun, 2013 | Pubmed ID: 23746975 Directed Differentiation and Functional Maturation of Cortical Interneurons from Human Embryonic Stem Cells Cell Stem Cell. May, 2013 | Pubmed ID: 23642365 CLP1 Links TRNA Metabolism to Progressive Motor-neuron Loss Nature. Mar, 2013 | Pubmed ID: 23474986 Analysis of Human Embryos from Zygote to Blastocyst Reveals Distinct Gene Expression Patterns Relative to the Mouse Developmental Biology. Mar, 2013 | Pubmed ID: 23261930 Developmental Bias in Cleavage-stage Mouse Blastomeres Current Biology : CB. Jan, 2013 | Pubmed ID: 23177476 SnapShot: Directed Differentiation of Pluripotent Stem Cells Cell. May, 2012 | Pubmed ID: 22632979 Erosion of Dosage Compensation Impacts Human IPSC Disease Modeling Cell Stem Cell. May, 2012 | Pubmed ID: 22560080 Impracticality of Egg Donor Recruitment in the Absence of Compensation Cell Stem Cell. Oct, 2011 | Pubmed ID: 21982228 Reprogramming Within Hours Following Nuclear Transfer into Mouse but Not Human Zygotes Nature Communications. 2011 | Pubmed ID: 21971503 Conversion of Mouse and Human Fibroblasts into Functional Spinal Motor Neurons Cell Stem Cell. Sep, 2011 | Pubmed ID: 21852222 Constructing and Deconstructing Stem Cell Models of Neurological Disease Neuron. May, 2011 | Pubmed ID: 21609821 Somatic Coding Mutations in Human Induced Pluripotent Stem Cells Nature. Mar, 2011 | Pubmed ID: 21368825 Reference Maps of Human ES and IPS Cell Variation Enable High-throughput Characterization of Pluripotent Cell Lines Cell. Feb, 2011 | Pubmed ID: 21295703 A Functionally Characterized Test Set of Human Induced Pluripotent Stem Cells Nature Biotechnology. Mar, 2011 | Pubmed ID: 21293464 Shushing Down the Epigenetic Landscape Towards Stem Cell Differentiation Development (Cambridge, England). Aug, 2010 | Pubmed ID: 20554719 Recipient Cell Nuclear Factors Are Required for Reprogramming by Nuclear Transfer Development (Cambridge, England). Jun, 2010 | Pubmed ID: 20463036 Sox17 Promotes Differentiation in Mouse Embryonic Stem Cells by Directly Regulating Extraembryonic Gene Expression and Indirectly Antagonizing Self-renewal Genes & Development. Feb, 2010 | Pubmed ID: 20123909 Progress Toward the Clinical Application of Patient-specific Pluripotent Stem Cells The Journal of Clinical Investigation. Jan, 2010 | Pubmed ID: 20051636 A Small-molecule Inhibitor of Tgf-Beta Signaling Replaces Sox2 in Reprogramming by Inducing Nanog Cell Stem Cell. Nov, 2009 | Pubmed ID: 19818703 Reprogramming After Chromosome Transfer into Mouse Blastomeres Current Biology : CB. Aug, 2009 | Pubmed ID: 19682906 Digital RNA Allelotyping Reveals Tissue-specific and Allele-specific Gene Expression in Human Nature Methods. Aug, 2009 | Pubmed ID: 19620972 Targeted Bisulfite Sequencing Reveals Changes in DNA Methylation Associated with Nuclear Reprogramming Nature Biotechnology. Apr, 2009 | Pubmed ID: 19330000 Optimal Timing of Inner Cell Mass Isolation Increases the Efficiency of Human Embryonic Stem Cell Derivation and Allows Generation of Sibling Cell Lines Cell Stem Cell. Feb, 2009 | Pubmed ID: 19200798 Human Embryonic Stem Cell-derived Motor Neurons Are Sensitive to the Toxic Effect of Glial Cells Carrying an ALS-causing Mutation Cell Stem Cell. Dec, 2008 | Pubmed ID: 19041780 Using Stem Cells and Reprogramming to Understand Disease Regenerative Medicine. Nov, 2008 | Pubmed ID: 18947303 Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons Science (New York, N.Y.). Aug, 2008 | Pubmed ID: 18669821 Mediators of Reprogramming: Transcription Factors and Transitions Through Mitosis Nature Reviews. Molecular Cell Biology. Jul, 2008 | Pubmed ID: 18568039 Short-circuiting Epiblast Development Cell Stem Cell. Aug, 2007 | Pubmed ID: 18371343 Developmental Reprogramming After Chromosome Transfer into Mitotic Mouse Zygotes Nature. Jun, 2007 | Pubmed ID: 17554301 Non-cell Autonomous Effect of Glia on Motor Neurons in an Embryonic Stem Cell-based ALS Model Nature Neuroscience. May, 2007 | Pubmed ID: 17435754 Dolly's Legacy: Human Nuclear Transplantation and Better Medicines for Our Children Cloning and Stem Cells. 2007 | Pubmed ID: 17386009 The Potential of Cell Fusion for Human Therapy Stem Cell Reviews. 2006 | Pubmed ID: 17848721 A Transcriptional Logic for Nuclear Reprogramming Cell. Aug, 2006 | Pubmed ID: 16923385 Ovulated Oocytes in Adult Mice Derive from Non-circulating Germ Cells Nature. Jun, 2006 | Pubmed ID: 16799565 Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells Science (New York, N.Y.). Aug, 2005 | Pubmed ID: 16123299 Nuclear Cloning, Epigenetic Reprogramming and Cellular Differentiation Novartis Foundation Symposium. 2005 | Pubmed ID: 16050253 Mice Cloned from Olfactory Sensory Neurons Nature. Mar, 2004 | Pubmed ID: 14990966 Derivation of Embryonic Germ Cells and Male Gametes from Embryonic Stem Cells Nature. Jan, 2004 | Pubmed ID: 14668819 Micromanipulating Dosage Compensation: Understanding X-chromosome Inactivation Through Nuclear Transplantation Seminars in Cell & Developmental Biology. Dec, 2003 | Pubmed ID: 15015742 Differentiation of F1 Embryonic Stem Cells into Viable Male and Female Mice by Tetraploid Embryo Complementation Methods in Enzymology. 2003 | Pubmed ID: 14696335 Incomplete Reactivation of Oct4-related Genes in Mouse Embryos Cloned from Somatic Nuclei Development (Cambridge, England). Apr, 2003 | Pubmed ID: 12620990 Nuclear Cloning, Stem Cells, and Genomic Reprogramming Cloning and Stem Cells. 2002 | Pubmed ID: 12630413 Abnormal Gene Expression in Cloned Mice Derived from Embryonic Stem Cell and Cumulus Cell Nuclei Proceedings of the National Academy of Sciences of the United States of America. Oct, 2002 | Pubmed ID: 12235366 Male and Female Mice Derived from the Same Embryonic Stem Cell Clone by Tetraploid Embryo Complementation Nature Biotechnology. May, 2002 | Pubmed ID: 11981557 Dnmt1 Overexpression Causes Genomic Hypermethylation, Loss of Imprinting, and Embryonic Lethality Molecular and Cellular Biology. Apr, 2002 | Pubmed ID: 11884600 Flp Recombinase Regulated LacZ Expression at the ROSA26 Locus Genesis (New York, N.Y. : 2000). Feb, 2002 | Pubmed ID: 11857816 मॉडलिंग स्टेम सेल व्युत्पंन सेरेब्रल Organoids का उपयोग इन विट्रो में विकासशील मानव मस्तिष्क के Zika वायरस संक्रमण Max R Salick*1, Michael F Wells*2,3, Kevin Eggan2,3, Ajamete Kaykas1 1Department of Neuroscience, Novartis Institutes for BioMedical Research, 2Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, 3Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University JoVE 56404 Neuroscience माउस oocytes में न्यूक्लियर ट्रांसफर Dieter Egli1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 116 Biology जमे हुए कक्ष से शुरू संस्कृति: मानव ES कोशिकाओं Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 86 Biology Passaging hues मानव भ्रूणीय स्टेम trypsin के साथ सेल लाइनों Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 49 Biology बर्फ़ीली मानव ES कक्ष Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 50 Biology
मॉडलिंग स्टेम सेल व्युत्पंन सेरेब्रल Organoids का उपयोग इन विट्रो में विकासशील मानव मस्तिष्क के Zika वायरस संक्रमण Max R Salick*1, Michael F Wells*2,3, Kevin Eggan2,3, Ajamete Kaykas1 1Department of Neuroscience, Novartis Institutes for BioMedical Research, 2Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, 3Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University JoVE 56404 Neuroscience
माउस oocytes में न्यूक्लियर ट्रांसफर Dieter Egli1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 116 Biology
जमे हुए कक्ष से शुरू संस्कृति: मानव ES कोशिकाओं Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 86 Biology
Passaging hues मानव भ्रूणीय स्टेम trypsin के साथ सेल लाइनों Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 49 Biology
बर्फ़ीली मानव ES कक्ष Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 50 Biology
