Articles by Kyle S. Nickey in JoVE
Teratom Generation i testiklerne Capsule Suzanne E. Peterson1, Ha T. Tran1, Ibon Garitaonandia1, Sangyoon Han1, Kyle S. Nickey1, Trevor Leonardo2, Louise C. Laurent3, Jeanne F. Loring1 1Department of Chemical Physiology, Scripps Research Institute, 2Department of Chemical Physiology, Scripps Research Institute, 3Department of Reproductive Medicine, University of California Humane pluripotente stamceller (hPSCs) har potentialet til at behandle et utal af forskellige sygdomme. Anvendeligheden af disse celler består i det faktum, at de kan differentiere til enhver celletype i legemet. Her beskriver vi teratom assay, som anvendes til at påvise pluripotence af hPSCs.
Other articles by Kyle S. Nickey on PubMed
Normal Human Pluripotent Stem Cell Lines Exhibit Pervasive Mosaic Aneuploidy PloS One. 2011 | Pubmed ID: 21857983 Human pluripotent stem cell (hPSC) lines have been considered to be homogeneously euploid. Here we report that normal hPSC--including induced pluripotent--lines are karyotypic mosaics of euploid cells intermixed with many cells showing non-clonal aneuploidies as identified by chromosome counting, spectral karyotyping (SKY) and fluorescent in situ hybridization (FISH) of interphase/non-mitotic cells. This mosaic aneuploidy resembles that observed in progenitor cells of the developing brain and preimplantation embryos, suggesting that it is a normal, rather than pathological, feature of stem cell lines. The karyotypic heterogeneity generated by mosaic aneuploidy may contribute to the reported functional and phenotypic heterogeneity of hPSCs lines, as well as their therapeutic efficacy and safety following transplantation.