Articles by Long Mei in JoVE
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters Long Mei1,2, Cheng Zhan2, Eric Erquan Zhang2 1PTN Joint Graduate Program, School of Life Sciences, Peking University, 2National Institute of Biological Sciences, Beijing This newly developed fluorescence-based technology enables long-term monitoring of the transcription of circadian clock genes in the suprachiasmatic nucleus (SCN) of freely moving mice in real-time and at a high temporal resolution.
Other articles by Long Mei on PubMed
Long-term in Vivo Recording of Circadian Rhythms in Brains of Freely Moving Mice Proceedings of the National Academy of Sciences of the United States of America. Apr, 2018 | Pubmed ID: 29610316 Endogenous circadian clocks control 24-h physiological and behavioral rhythms in mammals. Here, we report a real-time in vivo fluorescence recording system that enables long-term monitoring of circadian rhythms in the brains of freely moving mice. With a designed reporter of circadian clock gene expression, we tracked robust transcription reporter rhythms in the suprachiasmatic nucleus (SCN) of WT, , and mice in LD (12 h light, 12 h dark) and DD (constant darkness) conditions and verified that signals remained stable for over 6 mo. Further, we recorded transcriptional rhythms in the subparaventricular zone (SPZ) and hippocampal CA1/2 regions of WT mice housed under LD and DD conditions. By using a Cre-loxP system, we recorded and transcription rhythms specifically in vasoactive intestinal peptide (VIP) neurons of the SCN. Finally, we demonstrated the dynamics of and transcriptional rhythms in SCN VIP neurons following an 8-h phase advance in the light/dark cycle.