In JoVE (1)
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Articles by Mark Schmitt in JoVE
The Organoid Reconstitution Assay (ORA) for the Functional Analysis of Intestinal Stem and Niche Cells Matthias Schewe1, Andrea Sacchetti1, Mark Schmitt1, Riccardo Fodde1 1Department of Pathology, Erasmus MC Cancer Institute, Erasmus University Medical Center Intestinal organoid cultures are established from whole crypts and do not allow the analysis of self-renewal and differentiation in a cell-specific fashion. This protocol describes reconstitution of sorted stem (Lgr5+) and niche (Paneth) cells, which give rise to organoids while enabling their prior biochemical and genetic modification and functional analysis.