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Articles by Masahiro Yamazoe in JoVE
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Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping
Kensuke Ihara*1, Koji Sugiyama*1, Kentaro Takahashi*1, Masahiro Yamazoe1, Tetsuo Sasano2, Tetsushi Furukawa1
1Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 2Department of Biofunctional Informatics, Tokyo Medical and Dental University
This protocol describes the electrophysiological evaluation of murine atria utilizing an optical mapping system with a high temporal and spatial resolution, including dual recordings of the membrane voltage and Ca2+ transient under programmed stimulation through a specialized electrode catheter.
Other articles by Masahiro Yamazoe on PubMed
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Combined Analysis of Human and Experimental Murine Samples Identified Novel Circulating MicroRNAs As Biomarkers for Atrial Fibrillation
Circulation Journal : Official Journal of the Japanese Circulation Society.
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Pubmed ID: 29398686 Recent experimental studies have demonstrated that several microRNAs (miRNAs) expressed in atrial tissue promote a substrate of atrial fibrillation (AF). However, because it has not been fully elucidated whether these experimental data contribute to identifying circulating miRNAs as biomarkers for AF, we used a combined analysis of human serum and murine atrial samples with the aim of identifying these biomarkers for predicting AF.Methods and Results:Comprehensive analyses were performed to screen 733 miRNAs in serum from 10 AF patients and 5 controls, and 672 miRNAs in atrial tissue from 6 inducible atrial tachycardia model mice and 3 controls. We selected miRNAs for which expression was detected in both analyses, and their expression levels were changed in the human analyses, the murine analyses, or both. This screening identified 11 candidate miRNAs. Next, we quantified the selected miRNAs using a quantitative RT-PCR in 50 AF and 50 non-AF subjects. The individual assessment revealed that 4 miRNAs (miR-99a-5p, miR-192-5p, miR-214-3p, and miR-342-5p) were significantly upregulated in AF patients. A receiver-operating characteristics curve indicated that miR-214-3p and miR-342-5p had the highest accuracy. The combination of the 4 miRNAs modestly improved the predictive accuracy for AF (76% sensitivity, 80% specificity).
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