In JoVE (1)
Other Publications (1)
Articles by Matthew J. Wyatt in JoVE
Entrega direta da MIF Morpholinos Into the Otocyst Zebrafish por injeção e Eletroporação afeta o desenvolvimento da orelha interna Katie E. Holmes1, Matthew J. Wyatt2, Yu-chi Shen2, Deborah A. Thompson2,3, Kate F. Barald2,4 1Department of Veterinary Science, University of Wisconsin, Madison, 2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 3Present address: Department of Pulmonary Medicine, University of Michigan, Ann Arbor, MI, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI Um método para entregar morpholinos diretamente no otocyst zebrafish em 24hpf tem sido desenvolvido. Usando microinjeção de morpholinos no lúmen da vesícula ótica e eletroporação para efeito de penetração, fomos capazes de contornar o efeito de morpholinos sobre o cérebro e obter efeitos específicos para o ouvido interno.
Other articles by Matthew J. Wyatt on PubMed
Uniform Cell Seeding and Generation of Overlapping Gradient Profiles in a Multiplexed Microchamber Device with Normally-closed Valves Lab on a Chip. Nov, 2010 | Pubmed ID: 20835429 Generation of stable soluble-factor gradients in microfluidic devices enables studies of various cellular events such as chemotaxis and differentiation. However, many gradient devices directly expose cells to constant fluid flow and that can induce undesired responses from cells due to shear stress and/or wash out of cell-secreted molecules. Although there have been devices with flow-free gradients, they typically generate only a single condition and/or have a decaying gradient profile that does not accommodate long-term experiments. Here we describe a microdevice that generates several chemical gradient conditions on a single platform in flow-free microchambers which facilitates steady-state gradient profiles. The device contains embedded normally-closed valves that enable fast and uniform seeding of cells to all microchambers simultaneously. A network of microchannels distributes desired solutions from easy-access open reservoirs to a single output port, enabling a simple setup for inducing flow in the device. Embedded porous filters, sandwiched between the microchannel networks and cell microchambers, enable diffusion of biomolecules but inhibit any bulk flow over the cells.