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Articles by Matthew Steritz in JoVE
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq Phanidhar Kukutla1, Matthew Steritz1, Jiannong Xu1 1Department of Biology, New Mexico State University A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.