Articles by Muznah Khan in JoVE
マウスの耳としっぽの組織から生成初代線維芽細胞培養 Muznah Khan1, Stephan Gasser1,2 1Immunology Program, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore 我々は、マウスの耳と尾から初代線維芽細胞を生成するための簡単かつ迅速に実験手順を説明します。手順は、特別の動物の訓練を必要とせず、最大10日間室温で保存された耳からの線維芽細胞培養物の生成のために使用することができます。
Other articles by Muznah Khan on PubMed
The DNA Damage Response Induces Antigen Presenting Cell-like Functions in Fibroblasts European Journal of Immunology. Apr, 2014 | Pubmed ID: 24375454 The DNA damage response (DDR) alerts the immune system to the danger posed by DNA damage through the induction of damage-associated molecular pattern molecules, chemokines, and ligands for activating immune receptors such as lymphocyte function-associated antigen 1 (LFA-1), NKG2D, and DNAX accessory molecule 1 (DNAM-1). Here we provide evidence that OVA(257-264) -pulsed fibroblasts gain the ability to activate naïve OT-I CD8(+) T cells in response to DNA damage. The ability of fibroblasts to activate OT-I CD8(+) T cells depended on the upregulation of ICAM-1 on fibroblasts and DNAM-1 expression of CD8(+) T cells. OVA(257-264) -pulsed fibroblasts were able to induce a protective T-cell response against B16-OVA cells in a DDR-dependent manner. Hence, the DDR may alert the immune system to the presence of potentially dangerous cells by upregulating the expression of ligands that can induce the activation of innate and adaptive immune cells.