In JoVE (2)
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Articles by Nadezda A. Stepicheva in JoVE
High Throughput Microinjections of Sea Urchin Zygotes Nadezda A. Stepicheva1, Jia L. Song1 1Department of Biological Sciences, University of Delaware Microinjection is a common technique used to deliver DNA constructs, mRNAs, morpholino antisense oligonucleotides or other treatments into eggs, embryos, and cells of various species.
Primary Cell Cultures from the Mouse Retinal Pigment Epithelium Peng Shang1, Nadezda A. Stepicheva1, Stacey Hose1, J. Samuel Zigler, Jr.2, Debasish Sinha1,2 1Department of Ophthalmology, University of Pittsburgh School of Medicine, 2Wilmer Eye Institute, The Johns Hopkins University School of Medicine The retinal pigment epithelium (RPE) is a multi-functional epithelium of the eye. Here we present a protocol to establish primary cell cultures derived from the murine RPE.