Other Publications (6)
- Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy
- European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
- Annals of Transplantation
- European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes
- The Journal of International Medical Research
Articles by Pascal Paschenda in JoVE
Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo Tim R. Glowka1, Pascal Paschenda2, Michael Czaplik3, Jörg C. Kalff1, René H. Tolba2 1Department of Surgery, University of Bonn, 2Institute for Laboratory Animal Science & Experimental Surgery, RWTH Aachen University, 3Department of Anesthesiology, RWTH Aachen University Here we present a protocol to experimentally assess plasma coagulation in liver tissue in vivo. In a porcine model, microcirculation is examined by laser Doppler, coagulation depth is measured histologically, temperature at coagulation site by infrared thermometer and thermographic camera, and duct sealing effect is documented by burst pressure experiments.
Other articles by Pascal Paschenda on PubMed
Evaluation of the Novel Bipolar Vessel Sealing and Cutting Device BiCision® in a Porcine Model Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy. Nov, 2012 | Pubmed ID: 22455599 Energy-based technologies for tissue sealing and cutting are increasingly supplementing current standards used for haemostasis and dissection during laparoscopic surgery. For their safe and efficacious use in clinical practice, these instruments have to guarantee sufficient burst resistance and low thermal damage to adjacent tissue in combination with good cutting characteristics.
Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes. 2015 | Pubmed ID: 25341590 The isolated perfused porcine kidney (IPPK) model has been the method of choice for the early preclinical evaluation of kidney graft preservation techniques. The preferred reperfusion conditions have not yet been determined. Here, we examined the effects of pressure- or flow-controlled perfusion and oxygenation by pure oxygen or carbogen (95% O2/5% CO2) on normothermic reperfusion in the IPPK model.
Improved Preservation of Warm Ischemia-damaged Porcine Kidneys After Cold Storage in Ecosol, a Novel Preservation Solution Annals of Transplantation. Apr, 2015 | Pubmed ID: 25912926 Ecosol, an extracellular-type, colloid-based preservation solution, has recently been introduced for washout, cold storage, and machine perfusion preservation of kidney grafts. Here, we assessed the efficacy of Ecosol compared to the widely used Histidine-Tryptophan-Ketoglutarate solution (HTK) for 24-h cold storage preservation of warm ischemia-damaged kidney grafts.
Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes. 2016 | Pubmed ID: 27070724 The global shortage of donor organs for transplantation has necessitated the expansion of the organ pool through increased use of organs from less ideal donors. Venous systemic oxygen persufflation (VSOP) and oxygenated machine perfusion (OMP) have previously demonstrated beneficial results compared to cold storage (CS) in the preservation of warm-ischemia-damaged kidney grafts. The aim of this study was to compare the efficacy of VSOP and OMP for the preservation of warm-ischemia-damaged porcine kidneys using the recently introduced Ecosol preservation solution compared to CS using Ecosol or histidine-tryptophan-ketoglutarate solution (HTK).
Cyclooxygenase Inhibitors As a New Therapeutic Strategy in Small Bowel Transplantation Transplantation. Nov, 2016 | Pubmed ID: 27479158 The long-term outcome of intestinal transplantations is still not favorable, which is partly due to the intestinal susceptibility to ischemia. There are several indications that the inflammatory response to ischemia-reperfusion injury is mediated by cyclooxygenases and that their inhibition may be associated with improved organ function. The aim of this study was to analyze if cyclooxygenase (COX) inhibitors could improve the early posttransplant outcome after orthotopic small bowel transplantation.
Argon and Helium Plasma Coagulation of Porcine Liver Tissue The Journal of International Medical Research. Oct, 2017 | Pubmed ID: 28661266 Objective Argon plasma coagulation (APC) and helium plasma coagulation (HPC) are electrosurgical techniques that provide noncontact monopolar electrothermal haemostasis. Although these techniques have been widely used clinically during the last three decades, their in vivo effects on liver tissue remain unclear. Methods We investigated the effects of different power levels (10-100 W) of APC and HPC on liver coagulation in 11 Landrace pigs. Capillary blood flow and capillary blood flow velocity were recorded with a combined laser Doppler flowmeter and spectrophotometer. The temperature, clinical biochemical parameters, blood gas parameters, bile duct-sealing effect, and coagulation depth were measured. Results APC and HPC significantly reduced the capillary blood flow and capillary blood flow velocity compared with baseline flow. No significant temperature change was measured on the liver surface immediately after coagulation. The clinical biochemical and blood gas parameters were not different before and after coagulation. The coagulation depth was positively correlated with the device power setting. Conclusions These results prove that APC and HPC provide sufficient superficial haemostasis. No significant systemic effects occurred following coagulation. The depth of the coagulation effect can be controlled through selection of the output power level.