Other Publications (1)
Articles by R. John Holash in JoVE
Procedures for Rat in situ Skeletal Muscle Contractile Properties Brian R. MacIntosh1, Shane P. Esau1, R. John Holash1, Jared R. Fletcher1 1Faculty of Kinesiology, University of Calgary This video demonstrates the surgical preparation and procedures needed to study the contractile responses of the rat medial gastrocnemius muscle preparation in situ. This preparation allows measurement of skeletal muscle contractile properties under physiological conditions. The animal is anesthetized and the muscle is separated from surrounding tissue at its distal end. The Achilles tendon is attached to a force transducer, allowing measurement of the muscle’s contractile response at 37 degrees C with an intact circulation.