Articles by Rongfeng Cui in JoVE
Reprodução de vídeo automatizada interativo para estudos da comunicação animal Trisha Butkowski1, Wei Yan1, Aaron M. Gray2, Rongfeng Cui2, Machteld N. Verzijden2, Gil G. Rosenthal2 1Department of Visualization, Texas A&M University (TAMU), 2Department of Biology, Texas A&M University (TAMU) Reprodução de vídeo é uma técnica amplamente utilizada em comportamento animal. Criamos e avaliou um programa que se aplica com base em regras de reprodução, interativo 3-D de animações de computador em resposta a tempo real, dados sobre o comportamento automatizado assunto.
Other articles by Rongfeng Cui on PubMed
Functional Conservation and Diversification of Class E Floral Homeotic Genes in Rice (Oryza Sativa) The Plant Journal : for Cell and Molecular Biology. Mar, 2010 | Pubmed ID: 20003164 Mutant analyses in different eudicotyledonous flowering plants demonstrated that SEPALLATA-like MADS-box genes are required for the specification of sepals, petals, stamens and carpels, and for floral determinacy, thus defining class E floral organ identity genes. SEP-like genes encode MADS-domain transcription factors and constitute an angiosperm-specific gene clade whose members show remarkably different degrees of redundancy and sub-functionalization within eudicots. To better understand the evolutionary dynamics of SEP-like genes throughout the angiosperms we have knocked down SEP-like genes of rice (Oryza sativa), a distant relative of eudicots within the flowering plants. Plants affected in both OsMADS7 and OsMADS8 show severe phenotypes including late flowering, homeotic changes of lodicules, stamens and carpels into palea/lemma-like organs, and a loss of floral determinacy. Simultaneous knockdown of the four rice SEP-like genes OsMADS1, OsMADS5, OsMADS7 and OsMADS8, leads to homeotic transformation of all floral organs except the lemma into leaf-like organs. This mimics the phenotype observed with the sep1 sep2 sep3 sep4 quadruple mutant of Arabidopsis. Detailed analyses of the spatial and temporal mRNA expression and protein interaction patterns corresponding to the different rice SEP-like genes show strong similarities, but also gene-specific differences. These findings reveal conservation of SEP-like genes in specifying floral determinacy and organ identities since the separation of eudicots and monocots about 150 million years ago. However, they indicate also monocot-specific neo- and sub-functionalization events and hence underscore the evolutionary dynamics of SEP-like genes. Moreover, our findings corroborate the view that the lodicules of grasses are homologous to eudicot petals.
Maternal Control of Seed Size by EOD3/CYP78A6 in Arabidopsis Thaliana The Plant Journal : for Cell and Molecular Biology. Jan, 2012 | Pubmed ID: 22251317 Seed size in higher plants is coordinately determined by the growth of the embryo, endosperm and maternal tissue, but relatively little is known about the genetic and molecular mechanisms that set final seed size. We have previously demonstrated that Arabidopsis DA1 acts maternally to control seed size, with the da1-1 mutant producing larger seeds than wild type. Through an activation tagging screen for modifiers of da1-1, we have identified an enhancer of da1-1 (eod3-1D) in seed size. EOD3 encodes the Arabidopsis cytochrome P450/CYP78A6 and is expressed in most plant organs. Overexpression of EOD3 dramatically increases seed size of wild-type plants, while eod3-ko loss-of-function mutants form small seeds. The disruption of CYP78A9, the most closely related family member, synergistically enhances the seed size phenotype of eod3-ko mutants, indicating that EOD3 functions redundantly with CYP78A9 to affect seed growth. Reciprocal cross experiments show that EOD3 acts maternally to promote seed growth. eod3-ko cyp78a9-ko double mutants have smaller cells in the maternal integuments of developing seeds, whereas eod3-1D forms more and larger cells in the integuments. Genetic analyses suggest that EOD3 functions independently of maternal factors DA1 and TTG2 to influence seed growth. Collectively, our findings identify EOD3 as a factor of seed size control and give insight into how plants control their seed size. Â© 2012 The Authors. The Plant Journal Â© 2012 Blackwell Publishing Ltd.