Rosario Ocular Genomics Institute Massachusetts Eye and Ear- Harvard Medical School Biography Publications Institution JoVE Articles Rosario Rosario Fernandez-Godino, PhD, is an Assistant Professor of Ophthalmology at Harvard Medical School and a Principal Investigator at the Ocular Genomics Institute of Massachusetts Eye and Ear (Boston, MA). A native of Madrid, Spain, she earned her Ph.D. in genetics and cell biology from the Complutense University of Madrid in 2009. Since 2011, Dr. Fernandez-Godino has studied the molecular mechanisms underlying age-related macular degeneration (AMD).The Fernandez-Godino Laboratory utilizes cutting-edge molecular and cellular biology techniques to model pathologies associated with dysfunction of the retinal pigment epithelium (RPE) in vitro. Specifically, they use genome editing technology such as CRISPR/Cas9 to engineer induced pluripotent stem cells (iPSC) and further differentiate them into retinal cells. The cell-based models are valuable tools to study the mechanisms of pathology of inherited and complex eye diseases as well as to test potentialtreatments, including gene therapy. The ultimate goal of the Fernandez-Godino Lab is to identify effective targets that can be applied therapeutically to stop AMD progression. Publications AAV-Mediated Gene Augmentation Therapy Restores Critical Functions in Mutant PRPF31 IPSC-Derived RPE Cells Molecular Therapy. Methods & Clinical Development. Dec, 2019 | Pubmed ID: 31890732 C3a Triggers Formation of Sub-retinal Pigment Epithelium Deposits Via the Ubiquitin Proteasome Pathway Scientific Reports. 06, 2018 | Pubmed ID: 29946065 Alterations in Extracellular Matrix/Bruch's Membrane Can Cause the Activation of the Alternative Complement Pathway Via Tick-Over Advances in Experimental Medicine and Biology. Month, 2018 | Pubmed ID: 29721924 Changes in Extracellular Matrix Cause RPE Cells to Make Basal Deposits and Activate the Alternative Complement Pathway Human Molecular Genetics. 01, 2018 | Pubmed ID: 29095988 Characterization of LincRNA Expression in the Human Retinal Pigment Epithelium and Differentiated Induced Pluripotent Stem Cells PloS One. Month, 2017 | Pubmed ID: 28837677 Copy-number Variation is an Important Contributor to the Genetic Causality of Inherited Retinal Degenerations Genetics in Medicine : Official Journal of the American College of Medical Genetics. 06, 2017 | Pubmed ID: 27735924 Extracellular Matrix Alterations and Deposit Formation in AMD Advances in Experimental Medicine and Biology. Month, 2016 | Pubmed ID: 26427393 A Local Complement Response by RPE Causes Early-stage Macular Degeneration Human Molecular Genetics. Oct, 2015 | Pubmed ID: 26199322 Mouse Genetics and Proteomic Analyses Demonstrate a Critical Role for Complement in a Model of DHRD/ML, an Inherited Macular Degeneration Human Molecular Genetics. Jan, 2014 | Pubmed ID: 23943789 ניתוח יעיל ותרבות של תאי אפיתל פיגמנט רשתית העכבר הראשי Blanca Chinchilla1, Heran Getachew1, Rosario Fernandez-Godino1 1Ocular Genomics Institute of Massachusetts Eye and Ear, Harvard Medical School JoVE 62228 Biology
ניתוח יעיל ותרבות של תאי אפיתל פיגמנט רשתית העכבר הראשי Blanca Chinchilla1, Heran Getachew1, Rosario Fernandez-Godino1 1Ocular Genomics Institute of Massachusetts Eye and Ear, Harvard Medical School JoVE 62228 Biology