In JoVE (1)
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Articles by Sanket Pattanaik in JoVE
Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology Chase A. Arbra*1, Satish N. Nadig*1, Sarah Grace Dennis1, Sanket Pattanaik1, Heather A. Bainbridge1, J. Matthew Rhett1, Stephen A. Fann1, Carl Atkinson1, Michael J. Yost1 1Department of Surgery, Medical University of South Carolina Tissue-engineered renal constructs provide a solution for the organ shortage and deleterious effects of dialysis. Here, we describe a protocol to micro dissect murine kidneys for isolation of cortico-medullary segments. These segments are implanted into scaffold-free cellular constructs, forming renal organoids.