Other Publications (1)
Articles by Saray Gutiérrez in JoVE
Isolatie van Salmonella typhimurium-met Phagosomes van Macrophages Saray Gutiérrez1,2, Martina Wolke2, Georg Plum2, Nirmal Robinson1,2 1Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, 2Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne We beschrijven hier een eenvoudige en snelle methode voor de isolatie van Salmonella typhimurium-phagosomes van macrofagen bevattende coating van de bacteriën met biotine en daar.
Other articles by Saray Gutiérrez on PubMed
Salmonella Typhimurium Disrupts Sirt1/AMPK Checkpoint Control of MTOR to Impair Autophagy PLoS Pathogens. Feb, 2017 | Pubmed ID: 28192515 During intracellular infections, autophagy significantly contributes to the elimination of pathogens, regulation of pro-inflammatory signaling, secretion of immune mediators and in coordinating the adaptive immune system. Intracellular pathogens such as S. Typhimurium have evolved mechanisms to circumvent autophagy. However, the regulatory mechanisms targeted by S. Typhimurium to modulate autophagy have not been fully resolved. Here we report that cytosolic energy loss during S. Typhimurium infection triggers transient activation of AMPK, an important checkpoint of mTOR activity and autophagy. The activation of AMPK is regulated by LKB1 in a cytosolic complex containing Sirt1 and LKB1, where Sirt1 is required for deacetylation and subsequent activation of LKB1. S. Typhimurium infection targets Sirt1, LKB1 and AMPK to lysosomes for rapid degradation resulting in the disruption of the AMPK-mediated regulation of mTOR and autophagy. The degradation of cytosolic Sirt1/LKB1/AMPK complex was not observed with two mutant strains of S. Typhimurium, ΔssrB and ΔssaV, both compromising the pathogenicity island 2 (SPI2). The results highlight virulence factor-dependent degradation of host cell proteins as a previously unrecognized strategy of S. Typhimurium to evade autophagy.