In JoVE (1)
Other Publications (1)
Articles by Sergey Etchin in JoVE
In situ compressão de carga e de imagem não invasivos Correlative do Osso-Ligamento periodontal de dentes fibrosa Joint Andrew T. Jang1, Jeremy D. Lin1, Youngho Seo2, Sergey Etchin3, Arno Merkle3, Kevin Fahey3, Sunita P. Ho1 1Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California San Francisco, 2Department of Radiology and Biomedical Imaging, University of California San Francisco, 3Xradia Inc. Neste estudo, será discutido o uso de um dispositivo de carga acoplada situ com tomografia de raios de micro-X calculado para biomecânica fibrosos. Leituras experimentais identificáveis com uma mudança total na biomecânica incluem: 1) força reacionária versus deslocamento, deslocamento de dentes ou seja, dentro do alvéolo e sua resposta reacionária do carregamento, 2) tridimensional (3D) de configuração espacial e morfometria, ou seja geométrica relação do dente com o alvéolo, e 3) mudanças nas leituras 1 e 2, devido a uma mudança no eixo de carga, ou seja, cargas concêntricas ou excêntricas.
Other articles by Sergey Etchin on PubMed
Accurate Whole Human Genome Sequencing Using Reversible Terminator Chemistry Nature. Nov, 2008 | Pubmed ID: 18987734 DNA sequence information underpins genetic research, enabling discoveries of important biological or medical benefit. Sequencing projects have traditionally used long (400-800 base pair) reads, but the existence of reference sequences for the human and many other genomes makes it possible to develop new, fast approaches to re-sequencing, whereby shorter reads are compared to a reference to identify intraspecies genetic variation. Here we report an approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost. Single molecules of DNA are attached to a flat surface, amplified in situ and used as templates for synthetic sequencing with fluorescent reversible terminator deoxyribonucleotides. Images of the surface are analysed to generate high-quality sequence. We demonstrate application of this approach to human genome sequencing on flow-sorted X chromosomes and then scale the approach to determine the genome sequence of a male Yoruba from Ibadan, Nigeria. We build an accurate consensus sequence from >30x average depth of paired 35-base reads. We characterize four million single-nucleotide polymorphisms and four hundred thousand structural variants, many of which were previously unknown. Our approach is effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.