Stephen P. Muench School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology University of Leeds Biography Publications Institution JoVE Articles Stephen P. Muench has not added a biography. If you are Stephen P. Muench and would like to personalize this page please email our Author Liaison for assistance. Publications Tyrosine 288 in the Extracellular Domain of the Human P2X7 Receptor is Critical for Receptor Function Revealed by Structural Modeling and Site-directed Mutagenesis Proteins. Oct, 2021 | Pubmed ID: 34622987 On-grid and In-flow Mixing for Time-resolved Cryo-EM Acta Crystallographica. Section D, Structural Biology. Oct, 2021 | Pubmed ID: 34605427 Targeting K1.1 Channels in KCNT1-associated Epilepsy Trends in Pharmacological Sciences. 08, 2021 | Pubmed ID: 34074526 Structure of the Endocytic Adaptor Complex Reveals the Basis for Efficient Membrane Anchoring During Clathrin-mediated Endocytosis Nature Communications. 05, 2021 | Pubmed ID: 34001871 Cryo-EM Structure of Human Mitochondrial HSPD1 IScience. Jan, 2021 | Pubmed ID: 33506187 Structural Basis for Vascular Endothelial Growth Factor Receptor Activation and Implications for Disease Therapy Biomolecules. 12, 2020 | Pubmed ID: 33333800 Human TRPC5 Structures Reveal Interaction of a Xanthine-based TRPC1/4/5 Inhibitor with a Conserved Lipid Binding Site Communications Biology. 11, 2020 | Pubmed ID: 33230284 Contribution of Val/Ile87 Residue in the Extracellular Domain in Agonist-induced Current Responses of the Human and Rat P2X7 Receptors Purinergic Signalling. 12, 2020 | Pubmed ID: 33029714 Need for Speed: Examining Protein Behavior During CryoEM Grid Preparation at Different Timescales Structure (London, England : 1993). 11, 2020 | Pubmed ID: 32814033 Potent Tetrahydroquinolone Eliminates Apicomplexan Parasites Frontiers in Cellular and Infection Microbiology. 2020 | Pubmed ID: 32626661 Cryo-EM Structure and Molecular Dynamics Analysis of the Fluoroquinolone Resistant Mutant of the AcrB Transporter from Microorganisms. Jun, 2020 | Pubmed ID: 32585951 The Active Form of Quinol-dependent Nitric Oxide Reductase from is a Dimer IUCrJ. May, 2020 | Pubmed ID: 32431824 Structure-Based Identification and Characterization of Inhibitors of the Epilepsy-Associated K1.1 (KCNT1) Potassium Channel IScience. May, 2020 | Pubmed ID: 32408169 Structure of the Protective Nematode Protease Complex H-gal-GP and Its Conservation Across Roundworm Parasites PLoS Pathogens. 04, 2020 | Pubmed ID: 32271834 Sample Deposition Onto Cryo-EM Grids: from Sprays to Jets and Back Acta Crystallographica. Section D, Structural Biology. Apr, 2020 | Pubmed ID: 32254058 Styrene Maleic-acid Lipid Particles (SMALPs) into Detergent or Amphipols: An Exchange Protocol for Membrane Protein Characterisation Biochimica Et Biophysica Acta. Biomembranes. 05, 2020 | Pubmed ID: 31945320 Unprecedented Properties of Phenothiazines Unraveled by a NDH-2 Bioelectrochemical Assay Platform Journal of the American Chemical Society. 01, 2020 | Pubmed ID: 31880924 Styrene Maleic Acid Recovers Proteins from Mammalian Cells and Tissues While Avoiding Significant Cell Death Scientific Reports. 11, 2019 | Pubmed ID: 31767876 A Cryo-EM Grid Preparation Device for Time-resolved Structural Studies IUCrJ. Nov, 2019 | Pubmed ID: 31709058 Structural Insight into Eukaryotic Sterol Transport Through Niemann-Pick Type C Proteins Cell. 10, 2019 | Pubmed ID: 31543266 Dimeric Structures of Quinol-dependent Nitric Oxide Reductases (qNORs) Revealed by Cryo-electron Microscopy Science Advances. 08, 2019 | Pubmed ID: 31489376 Emerging Role of Electron Microscopy in Drug Discovery Trends in Biochemical Sciences. 10, 2019 | Pubmed ID: 31409563 LAT1 (SLC7A5) and CD98hc (SLC3A2) Complex Dynamics Revealed by Single-particle Cryo-EM Acta Crystallographica. Section D, Structural Biology. Jul, 2019 | Pubmed ID: 31282475 SMA-PAGE: A New Method to Examine Complexes of Membrane Proteins Using SMALP Nano-encapsulation and Native Gel Electrophoresis Biochimica Et Biophysica Acta. Biomembranes. 08, 2019 | Pubmed ID: 31150633 The Expanding Toolkit for Structural Biology: Synchrotrons, X-ray Lasers and CryoEM IUCrJ. Mar, 2019 | Pubmed ID: 30867914 Substrate Polyspecificity and Conformational Relevance in ABC Transporters: New Insights from Structural Studies Biochemical Society Transactions. 12, 2018 | Pubmed ID: 30514765 CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in Frontiers in Cellular and Infection Microbiology. 2018 | Pubmed ID: 30345257 Cryo-EM Structures of Complex I from Mouse Heart Mitochondria in Two Biochemically Defined States Nature Structural & Molecular Biology. 07, 2018 | Pubmed ID: 29915388 Approaches to Altering Particle Distributions in Cryo-electron Microscopy Sample Preparation Acta Crystallographica. Section D, Structural Biology. 06, 2018 | Pubmed ID: 29872006 X-ray and Cryo-EM Structures of Inhibitor-bound Cytochrome Complexes for Structure-based Drug Discovery IUCrJ. Mar, 2018 | Pubmed ID: 29765610 No Longer Hidden Secrets of Proton Pumping: The Resolution Revolution Enlightens V-ATPases Molecular Cell. 03, 2018 | Pubmed ID: 29547720 Elucidating the Structural Basis for Differing Enzyme Inhibitor Potency by Cryo-EM Proceedings of the National Academy of Sciences of the United States of America. 02, 2018 | Pubmed ID: 29434040 A Reconstitution Method for Integral Membrane Proteins in Hybrid Lipid-polymer Vesicles for Enhanced Functional Durability Methods (San Diego, Calif.). 09, 2018 | Pubmed ID: 29410153 Using a SMALP Platform to Determine a Sub-nm Single Particle Cryo-EM Membrane Protein Structure Biochimica Et Biophysica Acta. Feb, 2018 | Pubmed ID: 28993151 AtSPX1 Affects the AtPHR1-DNA-binding Equilibrium by Binding Monomeric AtPHR1 in Solution The Biochemical Journal. 10, 2017 | Pubmed ID: 28887383 Everyone Needs Good Neighbours - the Intricate Relationship Between the Acetylcholine-receptor Channel and Its Membrane Environment IUCrJ. Jul, 2017 | Pubmed ID: 28875018 Durable Vesicles for Reconstitution of Membrane Proteins in Biotechnology Biochemical Society Transactions. Feb, 2017 | Pubmed ID: 28202656 Conformational Changes During Human P2X7 Receptor Activation Examined by Structural Modelling and Cysteine-based Cross-linking Studies Purinergic Signalling. 03, 2017 | Pubmed ID: 28025718 The Conservation of Phosphate-binding Residues Among PHT1 Transporters Suggests That Distinct Transport Affinities Are Unlikely to Result from Differences in the Phosphate-binding Site Biochemical Society Transactions. 10, 2016 | Pubmed ID: 27911737 The Changing Landscape of Membrane Protein Structural Biology Through Developments in Electron Microscopy Molecular Membrane Biology. 03, 2016 | Pubmed ID: 27608730 Durable Proteo-hybrid Vesicles for the Extended Functional Lifetime of Membrane Proteins in Bionanotechnology Chemical Communications (Cambridge, England). Sep, 2016 | Pubmed ID: 27540604 Structure-based Identification and Characterisation of Structurally Novel Human P2X7 Receptor Antagonists Biochemical Pharmacology. 09, 2016 | Pubmed ID: 27481062 Artificial Membranes for Membrane Protein Purification, Functionality and Structure Studies Biochemical Society Transactions. 06, 2016 | Pubmed ID: 27284055 The Varied Functions of Aluminium-activated Malate Transporters-much More Than Aluminium Resistance Biochemical Society Transactions. 06, 2016 | Pubmed ID: 27284052 Rotating with the Brakes on and Other Unresolved Features of the Vacuolar ATPase Biochemical Society Transactions. 06, 2016 | Pubmed ID: 27284051 Pi Sensing and Signalling: from Prokaryotic to Eukaryotic Cells Biochemical Society Transactions. 06, 2016 | Pubmed ID: 27284040 A Method for Detergent-free Isolation of Membrane Proteins in Their Local Lipid Environment Nature Protocols. 07, 2016 | Pubmed ID: 27254461 Corrigendum to 'Methods to Account for Movement and Flexibility in Cryo-EM Data Processing' [Methods 100 (2016) 35-41] Methods (San Diego, Calif.). 07, 2016 | Pubmed ID: 27209563 Characterization of the Flexibility of the Peripheral Stalk of Prokaryotic Rotary A-ATPases by Atomistic Simulations Proteins. 09, 2016 | Pubmed ID: 27177595 Editorial Methods (San Diego, Calif.). 05, 2016 | Pubmed ID: 27060404 Methods to Account for Movement and Flexibility in Cryo-EM Data Processing Methods (San Diego, Calif.). 05, 2016 | Pubmed ID: 27016144 An Introduction to Sample Preparation and Imaging by Cryo-electron Microscopy for Structural Biology Methods (San Diego, Calif.). May, 2016 | Pubmed ID: 26931652 Extracellular and Luminal PH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes The Journal of Biological Chemistry. Apr, 2016 | Pubmed ID: 26912656 In Situ Formation of Magnetopolymersomes Via Electroporation for MRI Scientific Reports. Sep, 2015 | Pubmed ID: 26391797 Docking of Competitive Inhibitors to the P2X7 Receptor Family Reveals Key Differences Responsible for Changes in Response Between Rat and Human Bioorganic & Medicinal Chemistry Letters. Aug, 2015 | Pubmed ID: 26099538 Mechanism of Inhibition of Mouse Slo3 (KCa 5.1) Potassium Channels by Quinine, Quinidine and Barium British Journal of Pharmacology. Sep, 2015 | Pubmed ID: 26045093 Replace, Reuse, Recycle: Improving the Sustainable Use of Phosphorus by Plants Journal of Experimental Botany. Jun, 2015 | Pubmed ID: 25944926 Structure of the Vacuolar H+-ATPase Rotary Motor Reveals New Mechanistic Insights Structure (London, England : 1993). Mar, 2015 | Pubmed ID: 25661654 The Acanthamoeba Shikimate Pathway Has a Unique Molecular Arrangement and is Essential for Aromatic Amino Acid Biosynthesis Protist. Feb, 2015 | Pubmed ID: 25576842 The Use of SMALPs As a Novel Membrane Protein Scaffold for Structure Study by Negative Stain Electron Microscopy Biochimica Et Biophysica Acta. Feb, 2015 | Pubmed ID: 25450810 Understanding the Apparent Stator-rotor Connections in the Rotary ATPase Family Using Coarse-grained Computer Modeling Proteins. Dec, 2014 | Pubmed ID: 25174610 Non-synonymous Single Nucleotide Polymorphisms in the P2X Receptor Genes: Association with Diseases, Impact on Receptor Functions and Potential Use As Diagnosis Biomarkers International Journal of Molecular Sciences. Jul, 2014 | Pubmed ID: 25079442 PA1b Inhibitor Binding to Subunits C and E of the Vacuolar ATPase Reveals Its Insecticidal Mechanism The Journal of Biological Chemistry. Jun, 2014 | Pubmed ID: 24795045 ALOX12 in Human Toxoplasmosis Infection and Immunity. Jul, 2014 | Pubmed ID: 24686056 The Benzimidazole Based Drugs Show Good Activity Against T. Gondii but Poor Activity Against Its Proposed Enoyl Reductase Enzyme Target Bioorganic & Medicinal Chemistry Letters. Feb, 2014 | Pubmed ID: 24398298 Subunit Positioning and Stator Filament Stiffness in Regulation and Power Transmission in the V1 Motor of the Manduca Sexta V-ATPase Journal of Molecular Biology. Jan, 2014 | Pubmed ID: 24075871 Flexibility Within the Rotor and Stators of the Vacuolar H+-ATPase PloS One. 2013 | Pubmed ID: 24312643 Discrimination of Potent Inhibitors of Toxoplasma Gondii Enoyl-acyl Carrier Protein Reductase by a Thermal Shift Assay Biochemistry. Dec, 2013 | Pubmed ID: 24295325 Modification of Triclosan Scaffold in Search of Improved Inhibitors for Enoyl-acyl Carrier Protein (ACP) Reductase in Toxoplasma Gondii ChemMedChem. Jul, 2013 | Pubmed ID: 23776166 Development of a Triclosan Scaffold Which Allows for Adaptations on Both the A- and B-ring for Transport Peptides Bioorganic & Medicinal Chemistry Letters. Jun, 2013 | Pubmed ID: 23664871 Design, Synthesis, and Biological Activity of Diaryl Ether Inhibitors of Toxoplasma Gondii Enoyl Reductase Bioorganic & Medicinal Chemistry Letters. Apr, 2013 | Pubmed ID: 23453069 Novel N-benzoyl-2-hydroxybenzamide Disrupts Unique Parasite Secretory Pathway Antimicrobial Agents and Chemotherapy. May, 2012 | Pubmed ID: 22354304 Structural Divergence of the Rotary ATPases Quarterly Reviews of Biophysics. Aug, 2011 | Pubmed ID: 21426606 T. Gondii RP Promoters & Knockdown Reveal Molecular Pathways Associated with Proliferation and Cell-cycle Arrest PloS One. 2010 | Pubmed ID: 21124925 Archazolid A Binds to the Equatorial Region of the C-ring of the Vacuolar H+-ATPase The Journal of Biological Chemistry. Dec, 2010 | Pubmed ID: 20884613 Identification of T. Gondii Epitopes, Adjuvants, and Host Genetic Factors That Influence Protection of Mice and Humans Vaccine. May, 2010 | Pubmed ID: 20347630 Cryo-electron Microscopy of the Vacuolar ATPase Motor Reveals Its Mechanical and Regulatory Complexity Journal of Molecular Biology. Mar, 2009 | Pubmed ID: 19244615 Snelle rastervoorbereiding voor tijd-opgeloste cryo-elektronenmicroscopie David P. Klebl1, Frank Sobott2, Howard D. White3, Stephen P. Muench1 1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 3Department of Physiological Sciences, Eastern Virginia Medical School JoVE 62199 Biochemistry
Snelle rastervoorbereiding voor tijd-opgeloste cryo-elektronenmicroscopie David P. Klebl1, Frank Sobott2, Howard D. White3, Stephen P. Muench1 1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 3Department of Physiological Sciences, Eastern Virginia Medical School JoVE 62199 Biochemistry
