In JoVE (1)
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Articles by Svenja Feldmann in JoVE
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation Christoph Wiegreffe1, Svenja Feldmann1, Simeon Gaessler1, Stefan Britsch1 1Institute of Molecular and Cellular Anatomy, Ulm University This protocol provides instructions for direct observation of radially migrating cortical neurons. In utero electroporation, organotypic slice culture, and time-lapse confocal imaging are combined to directly and dynamically study the effects of overexpression or downregulation of genes of interest in migrating neurons and to analyze their differentiation during development.