Articles by Sylvain Coulombe In JoVE (1) Permeabilisering af adhæreret celler under anvendelse af en inertgas Jet Other Publications (2) Biomedical Engineering OnlineApplied Optics Articles by Sylvain Coulombe in JoVE Bioengineering Permeabilisering af adhæreret celler under anvendelse af en inertgas Jet Scott Cooper1, Paul Jonak1, Guillaume Chouinard-Pelletier1, Sylvain Coulombe1, Elizabeth Jones1, Richard L. Leask1,2 1Chemical Engineering, McGill University, 2Montreal Heart Institute Denne protokol beskriver en fremgangsmåde til midlertidig permeabilisering af adhærerende celler ved anvendelse af en inert gas jet. Denne teknik letter overførsel af genetisk materiale og biomolekyler i adhærente mammale celler ved anvendelse af mekaniske kræfter til at forstyrre plasmamembranen. Other articles by Sylvain Coulombe on PubMed Use of Inert Gas Jets to Measure the Forces Required for Mechanical Gene Transfection Biomedical Engineering Online. 2012 | Pubmed ID: 22963645 Transferring genes and drugs into cells is central to how we now study, identify and treat diseases. Several non-viral gene therapy methods that rely on the mechanical disruption of the plasma membrane have been proposed, but the success of these methods has been limited due to a lack of understanding of the mechanical parameters that lead to cell membrane permeability. Feasibility of Nanofluid-based Optical Filters Applied Optics. Mar, 2013 | Pubmed ID: 23458793 In this article we report recent modeling and design work indicating that mixtures of nanoparticles in liquids can be used as an alternative to conventional optical filters. The major motivation for creating liquid optical filters is that they can be pumped in and out of a system to meet transient needs in an application. To demonstrate the versatility of this new class of filters, we present the design of nanofluids for use as long-pass, short-pass, and bandpass optical filters using a simple Monte Carlo optimization procedure. With relatively simple mixtures, we achieve filters with
