Articles by Wenwen Kong in JoVE
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation Sheldon Lawrence II1,2,3, Quiying Pang2,3,4, Wenwen Kong2,3, Sixue Chen1,2,3,5 1Plant Molecular and Cellular Biology Program, University of Florida, 2Department of Biology, University of Florida, 3Genetics Institute (UFGI), University of Florida, 4Alkali Soil Natural Environmental Science Center, Northeast Forestry University, 5Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida This protocol describes a method of preparing enriched stomatal guard cells that is useful for physiological and other biological studies.
Other articles by Wenwen Kong on PubMed
Quantitative Proteomics Reveals a Role of JAZ7 in Plant Defense Response to Pseudomonas Syringae DC3000 Journal of Proteomics. | Pubmed ID: 29325990 Jasmonate ZIM-domain (JAZ) proteins are key transcriptional repressors regulating various biological processes. Although many studies have studied JAZ proteins by genetic and biochemical analyses, little is known about JAZ7-associated global protein networks and how JAZ7 contributes to bacterial pathogen defense. In this study, we aim to fill this knowledge gap by conducting unbiased large-scale quantitative proteomics using tandem mass tags (TMT). We compared the proteomes of a JAZ7 knock-out line, a JAZ7 overexpression line, as well as the wild type Arabidopsis plants in the presence and absence of Pseudomonas syringae DC3000 infection. Both pairwise comparison and multi-factor analysis of variance reveal that differential proteins are enriched in biological processes such as primary and secondary metabolism, redox regulation, and response to stress. The differential regulation in these pathways may account for the alterations in plant size, redox homeostasis and accumulation of glucosinolates. In addition, possible interplay between genotype and environment is suggested as the abundance of seven proteins is influenced by the interaction of the two factors. Collectively, we demonstrate a role of JAZ7 in pathogen defense and provide a list of proteins that are uniquely responsive to genetic disruption, pathogen infection, or the interaction between genotypes and environmental factors.
Metabolomics of Early Stage Plant Cell-Microbe Interaction Using Stable Isotope Labeling Frontiers in Plant Science. | Pubmed ID: 29922325 Metabolomics has been used in unraveling metabolites that play essential roles in plant-microbe (including pathogen) interactions. However, the problem of profiling a plant metabolome with potential contaminating metabolites from the coexisting microbes has been largely ignored. To address this problem, we implemented an effective stable isotope labeling approach, where the metabolome of a plant bacterial pathogen pv. tomato () DC3000 was labeled with heavy isotopes. The labeled bacterial cells were incubated with epidermal peels (EPs) with guard cells, and excessive bacterial cells were subsequently removed from the plant tissues by washing. The plant metabolites were characterized by liquid chromatography mass spectrometry using multiple reactions monitoring, which can differentiate plant and bacterial metabolites. Targeted metabolomic analysis suggested that DC3000 infection may modulate stomatal movement by reprograming plant signaling and primary metabolic pathways. This proof-of-concept study demonstrates the utility of this strategy in differentiation of the plant and microbe metabolomes, and it has broad applications in studying metabolic interactions between microbes and other organisms.