Articles by Yen-Li Wang in JoVE
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells Yen-Li Wang1,2, Adrienne Hong3, Tzung-Hai Yen1,4,5,6, Hsiang-Hsi Hong1,7,8 1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)2D3]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.
Other articles by Yen-Li Wang on PubMed
Potential Osteoinductive Effects of Calcitriol on the M-RNA of Mesenchymal Stem Cells Derived from Human Alveolar Periosteum BioMed Research International. | Pubmed ID: 28105418 This study characterized alveolar periosteum-derived mesenchymal stem cells (P-MSCs) and examined the hypothesis that 1,25-(OH)D (calcitriol) exerts osteoinductive effects on P-MSCs. The mRNA expressions of alkaline phosphatase (ALP), bone sialoprotein (BSP), core-binding factor alpha-1 (CBFA1), collagen-1 (Col-1), osteocalcin (OCN), and vitamin D receptor (VDR) were assessed after incubation with calcitriol for 2 weeks. Vitamin C as positive control (Vit. C-p) increased ALP and CBFA1 mRNA expression at both 1 and 2 weeks and increased BSP and Col-1 mRNA expression only at the first week. A concentration of 10 M calcitriol enhanced ALP, CBFA1, Col-1, and OCN mRNA expression at both weeks and BSP mRNA expression at the first week. Furthermore, 10 M calcitriol increased the mRNA expressions of all compounds at both weeks, except that of CBFA1 at the first week. 10 M calcitriol and Vit. C-p enhanced ALP activity at the second and third weeks. The results revealed that 10, 10, and 10 M calcitriol induced osteoinduction in alveolar P-MSCs by increasing ALP, CBFA1, Col-1, and OCN mRNA expression. A 10 M calcitriol yielded a higher mRNA expression than Vit. Cp on VDR and OCN mRNA expression at both weeks and on Col-1 mRNA at the second week.
Decreased Amount of Supporting Alveolar Bone at Single-Rooted Premolars Is Under Estimated by 2D Examinations Scientific Reports. | Pubmed ID: 28367999 The purpose of this study was to relate the proportions of bone-supported root length of a 2D view into the amount of a 3D bone-attached root surface area (BA-RSA) by using a dental laser scanner examination. White-light 3D scanning technology was used to probe 36 maxillary and 35 mandibular single-rooted premolars. The bone-supported height (BSH) and BA-RSA at designated levels (95-25%) were compared using statistical t tests. The 100% BSH and BA-RSA of the maxillary/mandibular premolars were 12.6 ± 1.60 mm/13.45 ± 1.47 mm (p
Noninvasive Structural and Microvascular Anatomy of Oral Mucosae Using Handheld Optical Coherence Tomography Biomedical Optics Express. | Pubmed ID: 29188097 In this study, we demonstrated the feasibility of using a handheld optical coherence tomography (OCT) for visualizations of the microstructural and microvascular features of various oral mucosal types. To scan arbitrary locations of the oral mucosa, a scanning probe was developed, composed of a probe body fabricated by a 3D printer, miniaturized two-axis galvanometer, relay lenses, and reflective prism. With a 3D printing technique, the probe weight and the system volume were greatly reduced, enabling the effective improvement of imaging artifacts from unconscious motion and system complexity. Additionally, in our design, the distal end of the probe can be switched to fit various oral conditions, and the optical parameters of the probe, such as the transverse resolution, working distance, and probe length can be easily varied. The results showed that the epithelium and lamina propria layers, as well as the fungiform papilla and salivary gland, were differentiated. Moreover, various microcirculation features at different mucosal sites were identified that are potentially effective indicators for the diagnosis of premalignant lesions. The demonstrated results indicate that the developed OCT system is a promising tool for noninvasive imaging of oral mucosae.