Zsuzsanna Izsvák Max Delbrück Center for Molecular Medicine Biography Publications Institution JoVE Articles Zsuzsanna Izsvák has not added a biography. If you are Zsuzsanna Izsvák and would like to personalize this page please email our Author Liaison for assistance. Publications Human Endogenous Retrovirus K Rec Forms a Regulatory Loop with MITF That Opposes the Progression of Melanoma to an Invasive Stage Viruses. 11, 2020 | Pubmed ID: 33202765 Curative in Vivo Hematopoietic Stem Cell Gene Therapy of Murine Thalassemia Using Large Regulatory Elements JCI Insight. Aug, 2020 | Pubmed ID: 32814708 Human Endogenous Retrovirus HERV-K(HML-2) RNA Causes Neurodegeneration Through Toll-like Receptors JCI Insight. 04, 2020 | Pubmed ID: 32271161 Corrigendum to "How to Tame an Endogenous Retrovirus: HERVH and the Evolution of Human Pluripotency" [Curr Opin Virol 25 (August 2017) 49-58] Current Opinion in Virology. 06, 2020 | Pubmed ID: 32265145 Nuclear Inclusions of Pathogenic Ataxin-1 Induce Oxidative Stress and Perturb the Protein Synthesis Machinery Redox Biology. 05, 2020 | Pubmed ID: 32145456 Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. 04, 2020 | Pubmed ID: 32122952 Author Correction: Human Somatic Cell Mutagenesis Creates Genetically Tractable Sarcomas Nature Genetics. Apr, 2020 | Pubmed ID: 32094913 A Single Amino Acid Switch Converts the Sleeping Beauty Transposase into an Efficient Unidirectional Excisionase with Utility in Stem Cell Reprogramming Nucleic Acids Research. 01, 2020 | Pubmed ID: 31777924 Critical Role for Piccolo in Synaptic Vesicle Retrieval ELife. 05, 2019 | Pubmed ID: 31074746 A Multiple Piccolino-RIBEYE Interaction Supports Plate-Shaped Synaptic Ribbons in Retinal Neurons The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. 04, 2019 | Pubmed ID: 30696732 In Vivo Hematopoietic Stem Cell Gene Therapy Ameliorates Murine Thalassemia Intermedia The Journal of Clinical Investigation. 02, 2019 | Pubmed ID: 30422819 Author Correction: Isolation and Cultivation of Naive-like Human Pluripotent Stem Cells Based on HERVH Expression Nature Protocols. Aug, 2019 | Pubmed ID: 30374153 Author Correction: Reprogramming Triggers Endogenous L1 and Alu Retrotransposition in Human Induced Pluripotent Stem Cells Nature Communications. 12, 2018 | Pubmed ID: 30568248 Ten Things You Should Know About Transposable Elements Genome Biology. 11, 2018 | Pubmed ID: 30454069 A Simple Approach for Multi-targeted ShRNA-mediated Inducible Knockdowns Using Sleeping Beauty Vectors PloS One. 2018 | Pubmed ID: 30339711 Integrating HDAd5/35++ Vectors As a New Platform for HSC Gene Therapy of Hemoglobinopathies Molecular Therapy. Methods & Clinical Development. Jun, 2018 | Pubmed ID: 29766024 Improved Molecular Platform for the Gene Therapy of Rare Diseases by Liver Protein Secretion European Journal of Medical Genetics. Nov, 2018 | Pubmed ID: 29704684 Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors Molecular Therapy : the Journal of the American Society of Gene Therapy. 04, 2018 | Pubmed ID: 29503198 A Combined HSC Transduction/Selection Approach Results in Efficient and Stable Gene Expression in Peripheral Blood Cells in Mice Molecular Therapy. Methods & Clinical Development. Mar, 2018 | Pubmed ID: 29255741 Gene Therapy with the Sleeping Beauty Transposon System Trends in Genetics : TIG. 11, 2017 | Pubmed ID: 28964527 Generation of an Immortalized Mesenchymal Stem Cell Line Producing a Secreted Biosensor Protein for Glucose Monitoring PloS One. 2017 | Pubmed ID: 28949988 Disturbed Placental Imprinting in Preeclampsia Leads to Altered Expression of DLX5, a Human-Specific Early Trophoblast Marker Circulation. Nov, 2017 | Pubmed ID: 28904069 How to Tame an Endogenous Retrovirus: HERVH and the Evolution of Human Pluripotency Current Opinion in Virology. 08, 2017 | Pubmed ID: 28750248 Going Non-viral: the Sleeping Beauty Transposon System Breaks on Through to the Clinical Side Critical Reviews in Biochemistry and Molecular Biology. 08, 2017 | Pubmed ID: 28402189 Engineering of PEDF-Expressing Primary Pigment Epithelial Cells by the SB Transposon System Delivered by PFAR4 Plasmids Molecular Therapy. Nucleic Acids. Mar, 2017 | Pubmed ID: 28325297 Long-Term Prevention of Congenital Thrombotic Thrombocytopenic Purpura in ADAMTS13 Knockout Mice by Sleeping Beauty Transposon-Mediated Gene Therapy Arteriosclerosis, Thrombosis, and Vascular Biology. 05, 2017 | Pubmed ID: 28254814 Transposition of the Bamboo Mariner-like Element Ppmar1 in Yeast Molecular Phylogenetics and Evolution. 04, 2017 | Pubmed ID: 28189615 Regulated Complex Assembly Safeguards the Fidelity of Sleeping Beauty Transposition Nucleic Acids Research. Jan, 2017 | Pubmed ID: 27913727 Sleeping Beauty Transposition: from Biology to Applications Critical Reviews in Biochemistry and Molecular Biology. Feb, 2017 | Pubmed ID: 27696897 Targeting of the Plzf Gene in the Rat by Transcription Activator-Like Effector Nuclease Results in Caudal Regression Syndrome in Spontaneously Hypertensive Rats PloS One. 2016 | Pubmed ID: 27727328 In Vivo Transduction of Primitive Mobilized Hematopoietic Stem Cells After Intravenous Injection of Integrating Adenovirus Vectors Blood. 11, 2016 | Pubmed ID: 27554082 Structural Determinants of Sleeping Beauty Transposase Activity Molecular Therapy : the Journal of the American Society of Gene Therapy. 08, 2016 | Pubmed ID: 27401040 Sleeping Beauty Transposase Structure Allows Rational Design of Hyperactive Variants for Genetic Engineering Nature Communications. Mar, 2016 | Pubmed ID: 27025571 A Helitron Transposon Reconstructed from Bats Reveals a Novel Mechanism of Genome Shuffling in Eukaryotes Nature Communications. Mar, 2016 | Pubmed ID: 26931494 Isolation and Cultivation of Naive-like Human Pluripotent Stem Cells Based on HERVH Expression Nature Protocols. Feb, 2016 | Pubmed ID: 26797457 Full-length Dysferlin Transfer by the Hyperactive Sleeping Beauty Transposase Restores Dysferlin-deficient Muscle Molecular Therapy. Nucleic Acids. Jan, 2016 | Pubmed ID: 26784637 Genome-wide Profiling Reveals Remarkable Parallels Between Insertion Site Selection Properties of the MLV Retrovirus and the PiggyBac Transposon in Primary Human CD4(+) T Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2016 | Pubmed ID: 26755332 Reprogramming Triggers Endogenous L1 and Alu Retrotransposition in Human Induced Pluripotent Stem Cells Nature Communications. Jan, 2016 | Pubmed ID: 26743714 Pluripotency and the Endogenous Retrovirus HERVH: Conflict or Serendipity? BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. Jan, 2016 | Pubmed ID: 26735931 Sleeping Beauty Transposon Vectors in Liver-directed Gene Delivery of LDLR and VLDLR for Gene Therapy of Familial Hypercholesterolemia Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2016 | Pubmed ID: 26670130 Identification and Re-addressing of a Transcriptionally Permissive Locus in the Porcine Genome Transgenic Research. Feb, 2016 | Pubmed ID: 26515985 Antiangiogenic and Neurogenic Activities of Sleeping Beauty-Mediated PEDF-Transfected RPE Cells In Vitro and In Vivo BioMed Research International. 2015 | Pubmed ID: 26697494 Sleeping Beauty Transposition Microbiology Spectrum. Apr, 2015 | Pubmed ID: 26104705 Retrotransposition Creates Sloping Shores: a Graded Influence of Hypomethylated CpG Islands on Flanking CpG Sites Genome Research. Aug, 2015 | Pubmed ID: 25995269 Genomic Analysis of Sleeping Beauty Transposon Integration in Human Somatic Cells PloS One. 2014 | Pubmed ID: 25390293 Primate-specific Endogenous Retrovirus-driven Transcription Defines Naive-like Stem Cells Nature. Dec, 2014 | Pubmed ID: 25317556 Gibbon Genome and the Fast Karyotype Evolution of Small Apes Nature. Sep, 2014 | Pubmed ID: 25209798 Human Satellite Cells Have Regenerative Capacity and Are Genetically Manipulable The Journal of Clinical Investigation. Oct, 2014 | Pubmed ID: 25157816 Human Somatic Cell Mutagenesis Creates Genetically Tractable Sarcomas Nature Genetics. Sep, 2014 | Pubmed ID: 25129143 Germline Transgenesis in Pigs by Cytoplasmic Microinjection of Sleeping Beauty Transposons Nature Protocols. Apr, 2014 | Pubmed ID: 24625780 Germline Transgenesis in Rabbits by Pronuclear Microinjection of Sleeping Beauty Transposons Nature Protocols. Apr, 2014 | Pubmed ID: 24625779 Germline Transgenesis in Rodents by Pronuclear Microinjection of Sleeping Beauty Transposons Nature Protocols. Apr, 2014 | Pubmed ID: 24625778 Suicidal Autointegration of Sleeping Beauty and PiggyBac Transposons in Eukaryotic Cells PLoS Genetics. Mar, 2014 | Pubmed ID: 24625543 Human Endogenous Retrovirus K (HML-2) RNA and Protein Expression is a Marker for Human Embryonic and Induced Pluripotent Stem Cells Retrovirology. 2013 | Pubmed ID: 24156636 Regulation of DNA Transposition by CpG Methylation and Chromatin Structure in Human Cells Mobile DNA. May, 2013 | Pubmed ID: 23676100 Correction: Reliable Transgene-independent Method for Determining Sleeping Beauty Transposon Copy Numbers Mobile DNA. Mar, 2013 | Pubmed ID: 23497459 Sleeping Beauty Transposon-based System for Cellular Reprogramming and Targeted Gene Insertion in Induced Pluripotent Stem Cells Nucleic Acids Research. Feb, 2013 | Pubmed ID: 23275558 Transposon-mediated Transgenesis, Transgenic Rescue, and Tissue-specific Gene Expression in Rodents and Rabbits FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Mar, 2013 | Pubmed ID: 23195032 Retargeting Sleeping Beauty Transposon Insertions by Engineered Zinc Finger DNA-binding Domains Molecular Therapy : the Journal of the American Society of Gene Therapy. Oct, 2012 | Pubmed ID: 22776959 Retargeting Transposon Insertions by the Adeno-associated Virus Rep Protein Nucleic Acids Research. Aug, 2012 | Pubmed ID: 22523082 The Sleeping Beauty Transposon Toolbox Methods in Molecular Biology (Clifton, N.J.). 2012 | Pubmed ID: 22367875 Sleeping Beauty Transposon Mutagenesis in Rat Spermatogonial Stem Cells Nature Protocols. Sep, 2011 | Pubmed ID: 21959235 Nonviral Gene Delivery with the Sleeping Beauty Transposon System Human Gene Therapy. Sep, 2011 | Pubmed ID: 21867398 Avoiding Cytotoxicity of Transposases by Dose-controlled MRNA Delivery Nucleic Acids Research. Sep, 2011 | Pubmed ID: 21609958 Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: an Overview Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21431720 Reliable Transgene-independent Method for Determining Sleeping Beauty Transposon Copy Numbers Mobile DNA. Mar, 2011 | Pubmed ID: 21371313 Sleeping Beauty Transposon Mutagenesis of the Rat Genome in Spermatogonial Stem Cells Methods (San Diego, Calif.). Apr, 2011 | Pubmed ID: 21193047 The Expanding Universe of Transposon Technologies for Gene and Cell Engineering Mobile DNA. Dec, 2010 | Pubmed ID: 21138556 Traducción De Transposición De La Bella Durmiente En Terapias Celulares: Retos Y Victorias BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. Sep, 2010 | Pubmed ID: 20652893 Generating Knockout Rats by Transposon Mutagenesis in Spermatogonial Stem Cells Nature Methods. Jun, 2010 | Pubmed ID: 20473302 Comparative Analysis of Transposable Element Vector Systems in Human Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Jun, 2010 | Pubmed ID: 20372108 A Novel Active Endogenous Retrovirus Family Contributes to Genome Variability in Rat Inbred Strains Genome Research. Jan, 2010 | Pubmed ID: 19887576 Efficient Stable Gene Transfer into Human Cells by the Sleeping Beauty Transposon Vectors Methods (San Diego, Calif.). Nov, 2009 | Pubmed ID: 19615447 Transposon-mediated Genome Manipulation in Vertebrates Nature Methods. Jun, 2009 | Pubmed ID: 19478801 Molecular Evolution of a Novel Hyperactive Sleeping Beauty Transposase Enables Robust Stable Gene Transfer in Vertebrates Nature Genetics. Jun, 2009 | Pubmed ID: 19412179 Targeted Gene Insertion for Molecular Medicine Journal of Molecular Medicine (Berlin, Germany). Nov, 2008 | Pubmed ID: 18607557 Transposition of a Reconstructed Harbinger Element in Human Cells and Functional Homology with Two Transposon-derived Cellular Genes Proceedings of the National Academy of Sciences of the United States of America. Mar, 2008 | Pubmed ID: 18339812 Transcriptional Activities of the Sleeping Beauty Transposon and Shielding Its Genetic Cargo with Insulators Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2008 | Pubmed ID: 18071335 Technology Transfer from Worms and Flies to Vertebrates: Transposition-based Genome Manipulations and Their Future Perspectives Genome Biology. 2007 | Pubmed ID: 18047686 Targeted Sleeping Beauty Transposition in Human Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Jun, 2007 | Pubmed ID: 17426709 The Ancient Mariner Sails Again: Transposition of the Human Hsmar1 Element by a Reconstructed Transposase and Activities of the SETMAR Protein on Transposon Ends Molecular and Cellular Biology. Jun, 2007 | Pubmed ID: 17403897 Transposons for Gene Therapy! Current Gene Therapy. Oct, 2006 | Pubmed ID: 17073604 Sleeping Beauty Transposase Modulates Cell-cycle Progression Through Interaction with Miz-1 Proceedings of the National Academy of Sciences of the United States of America. Mar, 2006 | Pubmed ID: 16537485 A Whole Lotta Jumpin' Goin' On: New Transposon Tools for Vertebrate Functional Genomics Trends in Genetics : TIG. Jan, 2005 | Pubmed ID: 15680506 Transposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates: a Contemporary Review of Experimental Strategies Methods in Molecular Biology (Clifton, N.J.). 2004 | Pubmed ID: 15020812 Development of Hyperactive Sleeping Beauty Transposon Vectors by Mutational Analysis Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2004 | Pubmed ID: 14759813 Sleeping Beauty Transposition: Biology and Applications for Molecular Therapy Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2004 | Pubmed ID: 14759798 Healing the Wounds Inflicted by Sleeping Beauty Transposition by Double-strand Break Repair in Mammalian Somatic Cells Molecular Cell. Jan, 2004 | Pubmed ID: 14759372 The Sleeping Beauty Transposable Element: Evolution, Regulation and Genetic Applications Current Issues in Molecular Biology. Jan, 2004 | Pubmed ID: 14632258 The Frog Prince: a Reconstructed Transposon from Rana Pipiens with High Transpositional Activity in Vertebrate Cells Nucleic Acids Research. Dec, 2003 | Pubmed ID: 14627820 The DNA-bending Protein HMGB1 is a Cellular Cofactor of Sleeping Beauty Transposition Nucleic Acids Research. May, 2003 | Pubmed ID: 12711676 Involvement of a Bifunctional, Paired-like DNA-binding Domain and a Transpositional Enhancer in Sleeping Beauty Transposition The Journal of Biological Chemistry. Sep, 2002 | Pubmed ID: 12082109 Preclinical Evaluation of a Cell-Based Gene Therapy Using the Sleeping Beauty Transposon System in Choroidal Neovascularization Molecular Therapy. Methods & Clinical Development. | Pubmed ID: 31890733 The Antibiotic-free PFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells Molecular Therapy. Nucleic Acids. | Pubmed ID: 29858090 Long-Term PEDF Release in Rat Iris and Retinal Epithelial Cells After Sleeping Beauty Transposon-Mediated Gene Delivery Molecular Therapy. Nucleic Acids. | Pubmed ID: 29246287 Aislamiento, cultivo e ingeniería genética de células epiteliales de pigmento primario de mamíferos para terapia génica no viral Thais Bascuas*1,2, Martina Kropp*1,2, Nina Harmening1,2, Brittany M. Wong1, Sandra Johnen3, Zsuzsanna Izsvák4, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine JoVE 62145 Biology Modificación genética basada en electroporación de células epiteliales pigmentarias humanas primarias utilizando el sistema de transposones de la Bella Durmiente Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3 1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute JoVE 61987 Medicine Inducción y análisis del estrés oxidativo en células epiteliales pigmentarias de la retina humana transfectadas por transposones de la bella durmiente Thais Bascuas1,2, Martina Kropp1,2, Nina Harmening1,2, Mohammed Asrih1, Zsuzsanna Izsvák3, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine JoVE 61957 Medicine
Aislamiento, cultivo e ingeniería genética de células epiteliales de pigmento primario de mamíferos para terapia génica no viral Thais Bascuas*1,2, Martina Kropp*1,2, Nina Harmening1,2, Brittany M. Wong1, Sandra Johnen3, Zsuzsanna Izsvák4, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine JoVE 62145 Biology
Modificación genética basada en electroporación de células epiteliales pigmentarias humanas primarias utilizando el sistema de transposones de la Bella Durmiente Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3 1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute JoVE 61987 Medicine
Inducción y análisis del estrés oxidativo en células epiteliales pigmentarias de la retina humana transfectadas por transposones de la bella durmiente Thais Bascuas1,2, Martina Kropp1,2, Nina Harmening1,2, Mohammed Asrih1, Zsuzsanna Izsvák3, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine JoVE 61957 Medicine
